Computer algorithm that predicts both acceptable and unacceptable private and public HLA class I antigens in highly sensitized patients.
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Algorithms↗
Biomedical subjects
D O Crowe
Publications and source records attributed to D O Crowe.
DTT-sensitive samples found on regional organ procurement trays.
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Cytotoxicity Tests, Immunologic↗
Kinetics of activation of benzo[a]pyrene for inhibition of interferon induction.
The induction of interferon-alpha/beta (IFN-alpha/beta) by polyribosinosinic-polyribocytidylic acid was inhibited when murine L-929 cells were pretreated with the carcinogen benzo[a]pyrene (BP). Exposure of L-929 cells for 24 hr to BP was required to inhibit IFN-alpha/beta induction. When the BP was activated to the ultimate carcinogenic form in an in vitro cell-free liver microsomal enzyme system, the exposure time of L-929 cells to BP required for inhibition of IFN induction was decreased to 3 hr. BP dosages required for inhibition of IFN induction were not altered when the BP was preactivated, and the degree of inhibition of interferon induction was related to the concentration of microsomal enzymes added to the cell-free activation system.
Animals↗
Kinetics of depression and recovery of murine hepatic cytochrome P-450 levels after treatment with the interferon inducer polyriboinosinic-polyribocytidylic acid.
In vivo treatment of randombred Swiss Webster mice with polyriboinosinic-polyribocytidylic acid (poly l X poly C) inhibited the induction of cytochrome P-450's by both 3-methylcholanthrene [(MCA) CAS: 56-49-5] and phenobarbitol [(PB) CAS: 50-06-6]. Concomitant treatments with poly l X poly C and a single dose of MCA inhibited the induction of P-450's for 24 hours and delayed the obtainment of the MCA-induced P-450 levels for approximately 48-72 hours. When cytochrome P-450 levels were induced by four successive daily treatments of MCA or PB and when poly l X poly C was given on only the 1st day, induction of P-450's was completely suppressed for 24 hours and obtainment of the maximal P-450 level was delayed by 72-96 hours. Treatment with poly l X poly C of animals preinduced for P-450's by four successive daily treatments with either PB or MCA decreased the P-450 content to the noninduced basal level within 24 hours. The effect was temporary in the MCA-treated mice since P-450 content recovered to the MCA-preinduced levels within 72 hours. PB-dependent P-450 induction was short lived, and no recovery occurred after poly l X poly C treatment of PB-preinduced mice. Reduced hepatic cytochrome P-450 contents correlated with decreased abilities of liver homogenates to metabolically activate benzo [a]pyrene (CAS: 50-32-8) and N-acetyl-2-aminofluorene (CAS: 53-96-3), as scored in an Ames Salmonella typhimurium revertant assay.
Animals↗