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D O Schmid

Publications and source records attributed to D O Schmid.

At least 19 recordsLinked to original sources
PubMed1998

An immuno-biological test of species specificity in a low-molecular organic extract (<10,000 D).

A pharmacological formulation derived from bovine blood was studied for species-specific substances, the problem being the low-molecular weight of its components. A detection of the species specificity for bovines was possible exclusively by the use of monovalent bovine-specific blood group sera. All other sera showed cross-reactions. Inhibition tests and blood-group-specific bovine test sera enabled us to identify the species specificity of bovines in the drug concentrate at dilutions up to 1/1,000 which are used as the basis for the production of the medication. The requirement of the German authorities (BGA) to examine the specificity of the identity in the medication can therefore be met by this immunobiological method.

Animals↗
PubMed1991

New blood group factors Es, Et and a new allele Ebdgjmt (E16) in the pig E blood group system.

By immunizing a miniature sow a monovalent reagent (later designated anti-Es) was prepared, detecting an alternative antigen to the Ej. Blood group factors Ej and Es thus form the fifth genetically closed E subsystem. Analyses of selected raw sera containing anti-Ej led to the determination of a further Ej subgroup (designated Et) which is antithetical, mutually excluding with the blood factor Er. New blood factor Et is inherited by alleles Edeghjmnt (= E9) and (= E16). The investigation in pig breeds kept in CSFR indicated that allele Edeghjmnt occurs in Black and White Prestice breed (qE9 = 0.076 +/- 0.010) while allele Edeghjmnr (= E14) only in miniature pigs (qE9 = 0.147 +/- 0.011) and in wild pigs. In an élite herd of Swedish Landrace kept in CSFR a new complex allele Ebdgjmt (= E16) was found. Its frequency in the population studied was 0.058 +/- 0.022.

Alleles↗
PubMed1991

A rare GC mutant in horses disclosed by isoelectric focusing and subsequent immunoprinting.

An apparent incompatibility in the GC/DBP system in a mare and her colt was found after classification by polyacrylamide gel electrophoresis. Because of this observation an analysis of the equine GC/DBP system by isoelectric focusing and subsequent immunoprinting was initiated. Further GC subtypes, however, were not encountered: the three phenotypes GC F, FS and S were also delineated by this method. The GC types in the case of disputed descent were dissolved: GC S was found in the mare and GC FS in her colt as well as in another of her male offspring. It is proposed that a rare GC mutant is responsible for this observation, namely a GC S* component which is readily classified by isoelectric focusing and immunoprinting and difficult to disclose by polyacrylamide gel electrophoresis.

Animals↗
PubMed1989

[Leukocyte migration inhibition after experimental trypanosome infection in cattle].

Blood serum from cattle experimentally infected with trypanosomes was tested for its activity influencing granulocyte migration. Pooled porcine granulocytes were used in the migration assay. The inhibitory migration activity observed in serum samples of trypanosome infected animals implies the presence of mediators of cellular immunity. Values of migration indices express reciprocal events of inhibitory and stimulating events in infected animals. This study allowed to follow at least some aspects of the very complex cellular immune system and its functioning. The observed differences in migration values in serum of each animal speak for an individual immunological capacity to defy trypanosome infections. The variability in occurrence of the first inhibitory activity after infection supports the view of individual responsiveness. Distinct differences in migration values were observed in Dahomey cattle after primary and secondary challenge with trypanosomes. Serum from reinfected animals exhibited a marked decrease in inhibitory activity as compared to samples obtained after first infection. These observations suggest a modulation of the individual immune response after multiple challenge with trypanosomes of the same strain. This study demonstrates the involvement of cell mediated immune responses to trypanosome infections.

Animals↗
PubMed1982

Chemical characterization of porcine blood serum components with A and SLA activity.

The porcine A blood group substance is found in the serum as a lipid and, additionally, in certain animals, as a glycoprotein. Swine lymphocyte antigens (SLA) occur in the serum only as glycoproteins. Heat treatment of the solid residue obtained by lipid extraction yielded a water-soluble fraction with low protein content, high A activity, but no SLA activity. Poly(glycosyl)ceramides with SLA activity do not occur in the serum; poly(glycosyl)ceramides with A activity cannot be excluded. Desialylation of protein fractions has no effect on A and SLA activity. Both A and SLA activities of protein fractions are stable to mild alkaline hydrolysis thus indicating N-glycosidic carbohydrate-peptide linkages.

ABO Blood-Group System↗
PubMed1977

PGM3 locus and its genetic polymorphism in lymphocytes of the pig.

PGM3 activity was investigated by means of horizontal starch gel electrophoresis. In the pig of the German Landrace three different phenotypes have been recognized: F, S and FS. Family studies suggest the occurrence of at least two alleles--PGM3F and the PGM3S at an autosomal locus.

Animals↗