[Alpha 1-antitrypsin - clinical and forensic aspects].
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Biomedical subjects
Publications and source records attributed to D Patzelt.
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The immunological comparison of human and equine Gc proteins showed partial identical reactions between both species. Immunizations of goats and rabbits with horse serum produced antisera able to recognize human Gc proteins.
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AFP concentrations were recorded from umbilical cord serum of 482 newborns, with 453 of them weighing more than 2.500 gram and 29 less than 2,501 gram. The AFP values thus found were correlated with the established clinical parameters of gestation, such as birth weight, length, circumference of head, biparietal skull diameter, circumference of chest, and gestationsal age by case history. - The closest correlation between any two of the above selected clinical parameters was found to exist between AFP concentration and gestational age by case history, whereas correlations were least close between AFP and biparietal diameter. Measurement of AFP in umbilical cord blood is considered to be merely a complementary criterion for assessment of gestational age by clinical parameter, because of too large scatter of individual data.
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The present paper deals with a method permitting the isolation of haptoglobin 2-2 from human serum or plasma. The haptoglobin is adsorbed to DEAE-cellulose at pH 5.1 by batching. The loaded cellulose is given into a column and the haptoglobin eluted by a 0.1 M to 0.15 M acetate buffer. The last step is a gel-chromatography on Sephadex G-200. Disc- and immunoelectrophoresis were used to test the purity. As by-product acid alpha1-glycoprotein can be obtained.
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The alpha-feto-protein (AFP) ist described on the basis of the available literature; its diagnostic use up to the present is outlined, and an immunological method of its quantitative determination is presented. The results obtained by quantitating the level of AFP in the cord serum of 50 mature newborns (-x = 9,9 mg/100 ml) are in good agreement with other reports.
By addition of EDTA-Na2 (Chelaplex III) to fresh sera the possibility for typing of C3-polymorphism is prolonged. Using a final concentration of 45.6 mmol EDTA-Na2/l the conversion is inhibited, and the C3 determination in serum by means of high voltage electrophoresis succeeds even after storage at room temperature during 6 weeks.
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