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D Pereira

Publications and source records attributed to D Pereira.

42 records · Page 3Linked to original sources
PubMed1981

Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer.

We have isolated a cDNA clone for one of the HLA-B locus alloantigens by hybridization with a 30-nucleotide-long DNA probe. The probe was isolated from a reverse transcriptase (RNA-dependent DNA nucleotidyltransferase)-catalyzed cDNA synthesis reaction on poly(A)-mRNA in which an oligonucleotide (5'-32P)dC-T-T-C-T-C-C-A-C-A-TOH served as a primer and in which dideoxynucleoside triphosphates were used to reduce the size and heterogeneity of the cDNA products. The desired cDNA clone was isolated from a library of recombinant cDNA clones in the plasmid pBR322. The partial nucleotide sequence of the cDNA clone corresponds to the amino acid sequence of HLA-B7 antigen. The approach described in this paper is extremely sensitive and may be useful in cloning other genes for which the corresponding mRNA is present at low levels. This cDNA clone is nearly full length and can be used to isolate and to study the genes within the HLA region and to obtain expression of HLA-B peptides in cells.

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PubMedDate not supplied

[Epidemiologic profile of transtelephonic electrocardiography in El Salvador during 1994].

UNLABELLED: A total of 3,787 electrocardiograms were obtained with transtelephonic monitors (TT-ECG) during 1994 in CardioTel of Central America, Nueva San Salvador, El Salvador. Each case was evaluated in terms of patient's risk and classified as high or low risk on basis of its clinical profile. There were 3,441 traces of low risk patients and 346 of high risk one. Female patients accounts for 64.7% of cases, with 35.3% corresponding to males. Of all the TT-ECG's a 52.3% were normal. Subjects older than 60 years (independently of risk had a greater probability of an abnormal TT-ECG; in the same way there was an statistically significative increase in the incidence of abnormal TT-ECG in high risk subjects compared with low risk ones. In respect to specific TT-ECG abnormalities against cardiovascular risk, a high risk condition implied a greater incidence of bundle branch blocks, signs of myocardial lesion/necrosis, left ventricular hypertrophy, sinus tachycardia and bradycardia, atrial fibrillation and premature ventricular and supraventricular complexes, with a p value of 0.05 of 0.001. CONCLUSIONS: TT-ECG is useful in the search of cardiovascular disease, regardless the presence or absence of symptoms. Age greater than 60 years and high cardiovascular risk implies a greater probability of abnormal TT-ECG.

Adult↗