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Biomedical subjects

D Perri

Publications and source records attributed to D Perri.

7 recordsLinked to original sources
PubMed2005

Effect of polysaccharides from Opuntia ficus-indica (L.) cladodes on the healing of dermal wounds in the rat.

In traditional medicine extracts of polysaccharide-containing plants are widely employed for the treatment of skin and epithelium wounds and of mucous membrane irritation. The extracts of Opuntia ficus-indica cladodes are used in folk medicine for their antiulcer and wound-healing activities. The present study describes the wound-healing potential of two lyophilized polysaccharide extracts obtained from O. ficus-indica (L.) cladodes applied on large full-thickness wounds in the rat. When topically applied for 6 days, polysaccharides with a molecular weight (MW)>10(4)Da from O. ficus-indica cladodes induce a beneficial effect on cutaneous repair in this experimental model; in particular the topical application of O. ficus-indica extracts on skin lesions accelerates the reepithelization and remodelling phases, also by affecting cell-matrix interactions and by modulating laminin deposition. Furthermore, the wound-healing effect is more marked for polysaccharides with a MW ranging 10(4)-10(6)Da than for those with MW>10(6)Da, leading us to suppose that the fine structure of these polysaccharides and thus their particular hygroscopic, rheologic and viscoelastic properties may be essential for the wound-healing promoter activity observed.

Animals↗
PubMed2000

The gamma-2-herpesvirus bovine herpesvirus 4 causes apoptotic infection in permissive cell lines.

Increasing evidence suggests that regulation of apoptosis in infected cells is associated with several viral infections. The gammaherpesvirus bovine herpesvirus 4 (BHV-4) has been shown to harbor genes with antiapoptotic potentialities. However, here we have demonstrated that productive infection of adherent, permissive cell lines by BHV-4 resulted in a cytopathic effect characterized by induction of apoptosis. This phenomenon was confirmed using different techniques to detect apoptosis and using different virus strains and cell targets. Apoptosis induced by BHV-4 was inhibited by (1) treatment with doses of heparin, which completely inhibited virus attachment and infectivity; (2) UV treatment, which completely abrogated infectivity; and (3) treatment with a dose of phosphonoacetic acid, which blocked virus replication. Virus-induced apoptosis was associated with a down-regulation of Bcl-2 expression and was reduced by Z-VAD-FMK, but not by Z-DEVD-FMK (caspase-3-specific) caspase inhibitors. Inhibition of apoptosis by Z-VAD-FMK treatment during infection did not modify virus yield. Therefore, despite the presence of antiapoptotic genes in its genoma, BHV-4 could complete its cycle of productive infection while inducing apoptosis of infected cells. This finding might have implications for the pathobiology of BHV-4 and other gammaherpesviruses in vivo.

Amino Acid Chloromethyl Ketones↗
PubMed1997

Herpes simplex virus 2 causes apoptotic infection in monocytoid cells.

Increasing evidence indicates that apoptosis can be associated with several viral infections. Here we demonstrate, that infection of monocytoid cells by Herpes simplex virus 2 (HSV-2) resulted, in time- and dose-dependent induction of apoptosis as an exclusive cytopathic effect. The phenomenon was confirmed using four different techniques. Conversely, apoptosis was not observed in the Vero cell line. Virus yield in monocytoid cells was delayed and reduced, although well detectable, in comparison with that observed in Vero cells. Nevertheless, released virions exhibited full infecting capability. Apoptosis induced by HSV-2 was not inhibited by cycloheximide and only partially by an UV-treatment which completely abrogated infectivity. Virus-induced apoptosis was partly inhibited by indomethacin and was associated with a down-regulation of Bcl-2. A similar, but less pronounced, apoptosis-inducing effect in monocytoid cells was also observed with HSV-1 infection. Depending on the target cells, therefore, HSV could complete a cycle of infection which is characterized by apoptosis of infected cells.

Journal Article↗
PubMed1992

[Poland syndrome].

A case of Poland's syndrome in a newborn is described. The syndrome is relatively unknown, especially in its epidemiological and aetiopathogenetic aspects. It's characterized by aplasia of the sternal head of the pectoral major muscle, hypoplasia of the upper extremities and homolateral aplasia of II, III, IV, V finger. Attention is drawn to the importance of reporting diagnosed cases in order to further our knowledge of the syndrome. The Authors are looking with a big interest the correlation between hair treatment and Poland's anomaly.

Adult↗
PubMed1990

Revolution by evolution.

The vision of an electronic healthcare record is achievable and pieces of the requisite technology are in place. More importantly, with their investments in networks and departmental and enterprise-wide systems, many hospitals have implemented data gathering, storage and analysis capabilities that position pieces of their electronic record of the future.

Diffusion of Innovation↗
PubMed1985

Adult age differences in visual search.

The visual search technique was used to assess adult age differences in visual information extraction. The study included three adult age groups. In Experiment 1, participants searched for targets embedded in a list of unrelated words. Targets were defined structurally, phonemically, or semantically. Search for structural targets was faster than search for phonemic and semantic targets. This was true for all three age groups. In Experiment 2, targets were embedded in prose. The oldest age group required additional time to detect each target type, but the largest age difference was associated with semantic search.

Adult↗