Effect of 2,4,5-trichlorophenoxy acetic acid (2,4,5-T) on the morphology of Klebsiella spp. from environmental and clinical sources.
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Biomedical subjects
Publications and source records attributed to D R Cullimore.
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A highly selective medium for the enumeration and isolation of Klebsiella pneumoniae and Klebsiella oxytoca was developed in which the typical colonies were convex and 1 to 2 mm in diameter. Their pigment was either a mucoid pink-red color or a more watery pale red with a dark red center. Relatively little colonial growth occurred for any other bacterial genera, and where such colonies did grow, they could be easily differentiated since the form was atypical. The medium already appears to have potential value as a means of assessing the efficiency of treating sewage and monitoring the microbiological quality of vegetables.
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Proteolysis in soil (Regina heavy clay) was monitored by the gelatinolytic etching of developed color film emulsion, using a film immersion-slide carrier technique. Etching followed a sigmoid curve to completion. A total of 11 herbicides were tested for their potential influence on this proteolytic function. Eight caused some inhibition, and two were stimulative at 200 microliter of applied herbicide per liter. Eptam was found to stimulate at low concentrations and inhibit at higher concentrations (greater than 1,000 microliter of applied Eptam per liter).
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