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D R Mottram

Publications and source records attributed to D R Mottram.

53 records · Page 3Linked to original sources
PubMed1971

Binding of N-(2-bromo ethyl)-N-ethyl-N 1 -naphthylmethylamine HBr (SY28) to the proteins of guinea-pig vas deferens.

1. Vasa deferentia from guinea-pigs were exposed to (14)C-SY28 HBr (5.32 x 10(-6)M) washed, lipid extracted, and broken up.2. The tissue was digested with papain, hydrolyzed with 6 N HCl, and gel filtration performed.3. Aliquots at each stage were used for paper chromatography and autoradiography.4. Three labelled spots (in addition to unbound 2-halogenoalkylamine) were located from acid hydrolysate and a fourth from gel filtrate.5. Selected amino-acids were complexed with (14)C-SY28 and the procedure repeated. The R(F) values were compared with those of the same amino-acids with and without 6 N HCl.6. Histidine, aspartic acid, arginine and serine gave a significant degree of binding of (14)C-SY28.7. The theoretical implications are discussed.

Animals↗
PubMed1971

3 H-noradrenaline-accumulating ability of cholinergic nerve terminals in cat sympathetic ganglia.

1. After incubation of pieces of cat posterior mesenteric ganglion in [(3)H]-noradrenaline solution, the localization of the isotope was assessed by electron-autoradiography.2. Significantly higher concentrations of [(3)H]-noradrenaline were found over acetylcholinesterase-positive axons terminal on ganglion cells than in adjacent background areas.3. [(3)H]-Noradrenaline was not accumulated by ganglion cells under these circumstances.4. The significance of these findings in relation to previous physiological and pharmacological investigation is discussed.

Acetylcholinesterase↗