Biomedical subjects

D Radulovic

Publications and source records attributed to D Radulovic.

10 recordsLinked to original sources

PubMed2006

Enlargement to a saccular aneurysm and subsequent rupture of infundibular widening of posterior communicating artery.

A small dilatation known as infundibular widening (IFw) is frequently seen (7%-25%) on the posterior communicating artery (PComA) at its origin from the internal carotid artery. Development and subsequent rupture of an aneurysm on a previously radiographically demonstrated IFw has rarely been documented. We present two patients who suffered from subarachnoid hemorrhage (SAH). Initial cerebral angiography demonstrated IFw on PComA. They were readmitted to the hospital 9 and 11 years later, after a new SAH. Repeated cerebral angiography revealed an aneurysm arising from the site where the IFw had been seen previously in both cases. The aneurysms were clipped with favorable outcome. This report adds two new cases documenting enlargement of PComAIFw into an aneurysm. Patients with PComAIFw, especially those who have experienced SAH, should be considered for periodic follow-up to rule out the development of an aneurysm over time.

Adult↗

PubMed2006

Traumatic delayed epidural hematoma.

OBJECT: Traumatic delayed epidural hematoma (DEH) can be defined as a hematoma that is insignificant or not present on the initial computerized tomography (CT) scan made after trauma but subsequent CT scan shows sizeable epidural bleeding. During a 3-year period we have treated a total of 96 epidural hematomas, eight (8.3 %) of which had a delayed onset. CASE REPORT: We present here an analysis of the eight patients with traumatic DEH which had a significant mass effect in all patients and required surgical evacuation. In three patients with mild head injury (GCS > 12) neurological deterioration indicated the necessity of repeating the CT scan and preceded the detection of DEH. In only one case of the five patients with moderate (8 < GCS < 13) and severe head injury (GCS < 9) was neurological deterioration the precursor of the DEH. All patients were immediately operated on after diagnosis. Postoperative outcome was favorable in all cases. CONCLUSIONS: DEHs are highly unpredictable and continue to cause diagnostic difficulty. Close observation for signs of clinical deterioration and repeat CT scan are the most important factors for early detection of DEH. Early diagnosis and prompt operation offers excellent results for DEHs.

Accidental Falls↗

PubMed2000

Determination of fluocortolone pivalate and fluocortolone hexanoate in suppositories using reverse-phase HPLC.

Fluocortolone and its esters are synthetic corticosteroids used topically in the treatment of various skin disorders. A method that can be successfully used for the separation and determination of fluocortolone pivalate and fluocortolone hexanoate in suppositories was developed. This method is based on reverse-phase HPLC on Supelcosil LC-18 (25 cm x 4.6 mm, 5 microns), using methanol-acetonitrile-water-glacial acetic acid (17:46:37:0.4 v/v/v/v) as mobile phase at a flow rate of 3.0 ml/min. Detection was carried out using a UV detector at 238 nm. The method developed was validated, and calibration curves were established dependent on peak area. The validated ranges for fluocortolone pivalate and fluocortolone hexanoate are 15-305 micrograms/ml (r = 0.9995) and 15-315 micrograms/ml (r = 0.9996), respectively. The limits of detection and the limits of quantification for both esters were also determined.

Administration, Topical↗

PubMed1997

Densitometric determination of metoprolol tartrate in pharmaceutical dosage forms.

This paper describes a simple densitometric method for the determination of metroprolol tartrate in tablets and ampoules. After separation on silica gel GF254 plates, using acetone-methanol-triethylamine as the mobile phase for the tablets and acetone-triethylamine for ampoules, the chromatographic zones corresponding to the spots of metoprolol were scanned. Quantitation was performed using a computer-controlled Camag TLC scanner and applying five-point calibration with polynomial regression. The calibration function was established in the ranges 1-28 micrograms for tablets and 1-9 micrograms for ampoules. The results obtained are precise and reproducible, with recovery values of 99.1-99.4%.

Adrenergic beta-Antagonists↗

PubMed1996

Phosphorus nuclear magnetic resonance spectroscopy: in vivo magnesium measurements in the skeletal muscle of normal subjects.

31P nuclear magnetic resonance spectroscopy was used to study the skeletal muscle of 33 normal males and 32 females. Free intracellular magnesium levels and the ratios of the phosphorus metabolites were determined. Males had significantly lower free magnesium levels (499.8 microM +/- 26.3 microM vs. 530.7 microM +/- 36.0 microM, P = 0.001, d.f. = 63, analysis of variance). The free magnesium level (rs = -0.5431, P = 0.001) and the phosphocreatin/Inorganic phosphate ratio in males (rs = -0.4102, P = 0.018), and the phosphocreatine/Inorganic phosphate ratio in females (rn = -0.4759, P = 0.009) fell with the increasing Minnesota Heart Health Program Questionnaire score.

Adult↗

PubMed1995

Spectrophotometric investigation of metoprolol-benzyl orange reaction and its application to the assay in pharmaceutical dosage forms.

A sensitive spectrophotometric method for the determination of metoprolol tartrate in tablets and ampoules is presented. Using spectrophotometric measurements, it was found that metoprolol tartrate and benzyl orange form a chloroform soluble ion-pair complex with an absorption maximum at 401 nm. The composition of the ion-pair complex was determined by applying Job's method to equimolar solutions of metoprolol tartrate: benzyl orange (1:2); molar absorptivity 7.39 x 10(3) mol-1 cm-1. Extraction of the ion-pair complex in chloroform was accomplished easily at a Britton-Robinson's buffered optimum pH = 5.2, mu = 0.1 mol/dm3. The relative stability constant, calculated according to the method of Sommer and Job's non-equimolar solutions, was log K = 9.72 (avg. value). Beer's law was obeyed up to 3.42 micrograms/ml of metoprolol tartrate (the detection limit was also 3.42 micrograms/ml). The precision of the method was checked at three different concentrations. The RSD (n = 7) varied from 0.51 to 2.03%. Reproducibility was examined by analysing Lopresor tablets and ampoules. Recoveries varied from 99-101%. The reported method, applied to the assay of metoprolol tartrate in tablets and ampoules, gives precise and reproducible results.

Azo Compounds↗

PubMed1992

Determination of sodium cromoglycate in pharmaceutical dosage forms using TLC-densitometry.

A TLC-UV densitometric method for the determination of sodium cromoglycate (SCG) in ophthalmic solutions, gels and capsules has been developed. After TLC separation of active substance on silica gel GF254 using methanol-water-ethylacetate (15:45:40 v/v/v) as the mobile phase, densitometric measurements were performed with HPTLC scanner at 254 nm. The proposed method is rapid and simple, free from interference by adjuvants and can be suggested for the routine analysis of sodium cromoglycate.

Capsules↗

PubMed1992

Investigation of Fe(III) chloride as a colour reagent for the spectrophotometric determination of bumetanide in the pure form and in preparations.

The formation and stability of the complex between bumetanide (3-butylamino-4-phenoxy-5-sulphamoylbenzoic acid) and Fe(III) ion was studied. It has been found that bumetanide reacts with Fe(III) chloride in the presence of ammonium thiocyanate at the pH 1.83-1.92 to form a violet complex soluble in chlorophorm, with the maximum absorbance at 514.5 nm. At the optimum experimental conditions the conditional stability constant of the complex was found to be log K' = 7.34. The Beer's law was obeyed up to the 160 microg x cm(-3) and the relative standard deviation varied from 0.406% to 0.475% (n = 10). The proposed method was found to be suitable for the accurate and sensitive analysis of bumetanide as a pure compound and from tables and ampoules.

Bumetanide↗

PubMed1986

Potential acuity meter for predicting visual acuity after Nd:YAG posterior capsulotomy.

We studied 30 patients with opacifications of the posterior capsule to determine if the potential acuity meter (PAM) could accurately predict final visual outcome after Nd:YAG discussion. The final visual acuity was within one line of the PAM prediction in 22 of 30 patients (73%), better by two or more lines in seven patients (23%), and worse in one patient (4%) by two lines. Although in thicker capsules the final acuity was occasionally better than the PAM prediction, the rates of false negative and false positive predictions were very low. Mild cystoid macular edema (3 patients), age-related macular degeneration (3 patients), intraocular lens status, and level of initial acuity did not diminish PAM accuracy. The PAM effectively predicts final visual acuity after YAG posterior capsulotomy, when used in a patient, unhurried manner.

Adult↗

PubMedDate not supplied

The relationship between fibromyalgia and interstitial cystitis.

Interstitial cystitis (IC) is a relatively uncommon and enigmatic disorder characterized by pain in the bladder and pelvic region, typically accompanied by urinary urgency and frequency. Fibromyalgia is a more common disorder, with the prominent symptoms being diffuse musculoskeletal pain and fatigue, and it has been well established that there is substantial clinical overlap between fibromyalgia and chronic fatigue syndrome (CFS). Although genitourinary and musculoskeletal symptoms predominate in IC and fibromyalgia respectively, both disorders share a number of features, including similar demographics, "allied conditions" (e.g. irritable bowel syndrome, headaches, etc.), natural history, aggravating factors, and efficacious therapy. We hypothesized that there was substantial clinical overlap between fibromyalgia and IC, and examined cohorts of individuals with these two disorders in parallel, to compare the spectrum of symptomatology. Sixty fibromyalgia patients, 30 IC patients, and 30 age-matched healthy controls were questioned regarding current symptomatology. A dolorimeter examination was also performed in the three groups to assess peripheral nociception. We found that the frequency of current symptoms was very similar for the fibromyalgia and IC groups. Both the fibromyalgia and IC patients displayed increased pain sensitivity when compared to healthy individuals, at both tender and control points. These data suggest that IC and fibromyalgia have significant overlap in symptomatology, and that IC patients display diffusely increased peripheral nociception, as is seen in fibromyalgia. Although central mechanisms have been suspected to contribute to the pathogenesis of fibromyalgia for some time, we speculate that these same types of mechanisms may be operative in IC, which has traditionally been felt to be a bladder disorder.

Adult↗