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D Reali

Publications and source records attributed to D Reali.

27 records · Page 2Linked to original sources
PubMedDate not supplied

A recombinant yeast strain as a short term bioassay to assess estrogen-like activity of xenobiotics.

The study investigated the responsiveness of a recombinant Saccharomyces cerevisiae yeast strain to screen estrogen-like chemicals in order to use it as a first level short-term bioassay for environmental preventive medicine purposes. We used the yeast strain RMY326 ER-ERE, containing the human estrogen receptor alpha and a reporter gene lacZ encoding the enzyme b-galactosidase. We tested the natural steroid hormone 17beta-estradiol, diethylstilbestrol, colchicine, some phytoestrogens and pesticides, and organochlorine insecticides. The relative inductive efficiency (RIE) and EC50 values confirmed the sensitivity and specificity of the yeast strain. A statistically significant reproducibility of results in repeated experiments was assessed.

Biological Assay↗
PubMedDate not supplied

[Mycobacteria in swimming pool water and the meaning of microbiological conventional indicators].

Monitoring program of hygienic quality water in twelve public swimming pools was performed. Legally required microbiological indicator parameters of safety for gastrointestinal illness were measured besides the analyses of Pseudomonas spp. and Staphylococcus spp. prevalence, frequency of recovery and number of nontuberculous mycobacteria. We detected positive samples for coliforms at lower rate (29.3%) than Pseudomonas (75.5%), Staphylococcus spp. (46%) and Mycobacteria (59.4%). We pointed out statistically significant correlation (r=0.67 p=0.0001) between Mycobacteria and Pseudomonas so we think that the latter might be a good predictive marker. As 82% of samples had free chlorine residual within the limits stated by Italian Laws, the efficacy of chlorination to prevent risk of infectious diseases transmission by route other than gastroenteric was discussed. A revision of both the sanitary significance of conventional microbial parameters and the related regulations appears necessary.

Mycobacterium↗
PubMedDate not supplied

[Isolation of halophilic vibrios as possible agents of food poisoning (author's transl)].

Seventy-six halophilic vibrio strains were isolated from different marine sources such as coastal waters, fishes and mussels. Twenty strains were identified as Vibrio parahaemolyticus, 44 as Vibrio alginolyticus and 12 other strains could not be classified because of their atypical biochemical activities. Further investigations are necessary in order to assess if the present strains of V. parahaemolyticus can have a role in food poisoning outbreaks or human sporadic enteritis in our geographic area.

Food Contamination↗
PubMedDate not supplied

[Presence of salmonellae, E. coli and their homologous phages in the seawater].

Comparative experiments were carried out on samples of sea water at different degrees of natural pollution. The aim of such experiments was the quantitative estimation of coliforms and salmonellae and their respective phages. The results obtained show that it is thus possible to get better informations regarding the nature and degree of water pollution, than these obtained by the E. coli count alone.

Coliphages↗