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Biomedical subjects

D Renner

Publications and source records attributed to D Renner.

At least 19 recordsLinked to original sources
PubMed1992

Coincidence of primary myelodysplastic syndrome and non-Hodgkin lymphoma.

A 60-year-old patient is presented with primary myelodysplastic syndrome classified according to the criteria of the French-American-British classification as chronic myelomonocytic leukemia and with a hyperviscosity syndrome. We found a monoclonal gammopathy with a very high serum immunoglobulin M level. There is strong evidence for coincidence of myelodysplastic syndrome and Waldenström's macroglobulinemia in this patient.

Humans↗
PubMed1990

[Acute unilateral blindness as the initial symptom of meningeosis lymphomatosa].

The development of Meningeosis lymphomatosa is a rare event even in high grade Non-Hodgkin's lymphoma, often without any typical clinical signs. We report a case, where a unilateral sudden loss of vision was the first symptom of meningeosis lymphomatosa and indicated a generalized relapse in the patient, who seemed so far to be in complete remission.

Abdominal Neoplasms↗
PubMed1988

Megakaryocyte polyploidy and maturation in chronic granulocytic leukemia.

To understand abnormal platelet production in chronic granulocytic leukemia, polyploidization and maturation of megakaryocytes in 10 patients were studied using a technique which allows sequential immunofluorescence identification by a monoclonal platelet antibody (C17), cytophotometric determination of the relative DNA content and cytological characterization of megakaryocytes in panoptically stained smears. Compared to normal conditions the proportion of diploid promegakaryocytes was not increased, suggesting an undisturbed influx of progenitor cells into the megakaryocytic cell compartment. Small tetraploid (4c) megakaryocytes undergo maturation without further polyploidization, the so-called microkaryocytes being mature rather than immature cells. Most of the megakaryocytes show rhythmical polyploidization only up to octoploid (8c) level, indicating the inability to produce high-polyploidy cells.

Adult↗
PubMed1987

A quantitative receptor assay for "digitalis-like" compounds in serum. Demonstration of raised concentrations in essential hypertension and correlation with arterial blood pressure.

The influence of serum from patients with essential hypertension on [3H]ouabain binding to isolated (Na+ +K+)-ATPase and on the reactivity with digoxin-specific antibodies was investigated. [3H]Ouabain binding to (Na+ +K+)-ATPase was significantly decreased (P less than 0.001) by sera of 18 hypertensive patients (34.9 +/- 1.5 pmol/U) as compared with 22 normotensive controls (43.8 +/- 1.2 pmol/U). The factor, whose concentration is increased in the serum of patients with essential hypertension, competed with [3H]ouabain at isolated (Na+ +K+)-ATPase. Therefore, it was possible to quantify this "digitalis-like" factor with a receptor assay in ouabain equivalents. Three times higher mean serum levels were found in hypertensive patients (234.8 +/- 48.7 nM) than in normotensive controls (76.3 +/- 9.3 nM). Deproteinization of the sera by ultrafiltration through steroid-adsorbing membranes and by boiling of acidified sera for 10 min led to a significant reduction of the inhibitory activity and to the complete loss of a difference between the sera of normotensives and hypertensives. After deproteinization by boiling for 15 min, sera of normotensives showed levels of "digitalis-like" compounds of 16.53 +/- 2.15 nM and hypertensives of 41.65 +/- 8.41 nM (P less than 0.05). Though significantly elevated concentrations of "digitalis-like" factor were measured with the receptor assay, no significant increase of digoxin-like activity could be detected with digoxin-specific antibodies in untreated serum.

Adolescent↗
PubMed1987

Diploid and tetraploid precursors of megakaryocytes in normal human bone marrow detected by immunofluorescence.

A sequential preparation method is described which allows immunological identification, morphological characterization, cytophotometric determination of relative DNA content of the megakaryocyte lineage as well as quantitation of megakaryocyte precursors in human bone marrow aspirates. We compared several monoclonal (anti-GP IIIa and HD 19) and polyclonal (A225, RAHPS) antiplatelet antibodies for immunofluorescent staining. Among the identified cells, a small number of cells showing a diploid and tetraploid DNA content were found which must be regarded as promegakaryoblasts, representing 2.5-4.7% of all megakaryocytes. The heterogenous morphology of these precursors in panoptically stained smears is described.

Bone Marrow Cells↗
PubMed1987

Sodium pump inhibitor in the serum of patients with essential hypertension and its partial purification from hemofiltrate.

A 3-fold higher concentration of "endogenous digitalis" is found in the serum of patients with essential hypertension than in the serum of normotensives, whose concentration was determined in 22 normotensive probands by an receptor assay using isolated (Na+ + K+)-ATPase as 76.3 +/- 9.3 nM ouabain equivalents. Since the concentration of "endogenous digitalis" was 7-19 fold higher in 2 patients, who had become uremic due to a malignant hypertension and since their serum levels fell 3-fold by hemodialysis, the hemofiltrate was used for partial purification of the substance. This was possible by hydrophobic chromatography on Amberlite XAD-2, octadecyl-coated silica gel, anion exchange chromatography and affinity chromatography on membrane-bound (Na+ + K+)-ATPase. The partially purified substance behaved like the material described by Cloix et al. (1985) Biochem. Biophys. Res. Commun. 131:1234-1240 and competed with digoxin for digoxin antibodies. Ascorbic acid isolated on the search for an inhibitor of (Na+ + K+)-ATPase from beef brain inhibited [3H]ouabain binding due to a decrease of the ouabain binding sites by a reduction of a group within the ATP binding site of the enzyme. Unsaturated fatty acids claimed to be "endogenous digitalis (Tamura et al. [1985] J. Biol. Chem. 260:9672-9677)" also lowered the capacity of the cardiac glycoside binding site but did not compete with ouabain.

Animals↗
PubMed1986

[Carcinoid].

Explore the source record for details and available documents.

Aged↗
PubMed1986

Blood pressure in essential hypertension correlates with the concentration of a circulating inhibitor of the sodium pump.

The influence of serum from patients with essential hypertension on the sodium efflux rate constants of human lymphocytes and on the activity of isolated (Na+ + K+)-ATPase was investigated. The ouabain-sensitive sodium efflux rate constant was significantly decreased (p less than 0.001) in the sera of 19 hypertensives (1.92 +/- 0.11 h-1) compared with the sera of 30 normotensives (2.44 +/- 0.07 h-1). The ouabain-insensitive sodium efflux was unaffected. These results corresponded with a significant difference (p less than 0.005) of (Na+ + K+)-ATPase activity (1.03 +/- 0.04 mU/ml and 0.079 +/- 0.06 mU/ml), when an isolated (Na+ + K+)-ATPase was incubated with the sera of 22 normotensives or 18 hypertensives. Both the rate constant of ouabain-sensitive sodium efflux and the (Na+ + K+)-ATPase activity correlated significantly with the diastolic and systolic blood pressure (p less than 0.001). These data, therefore, demonstrated the close relationship between essential hypertension and the concentration of a circulating inhibitor of the sodium pump.

Adult↗
PubMed1985

Suppression of antibody response and prolongation of skin graft survival by multiple blood transfusions in the rat.

The effect of blood transfusions (BT) on antibody response and skin graft survival was studied in the strongly MHC-incompatible BN and LEW combination. One-to-three BT induced high titer antibodies. Additional BT, however, led to a decrease of antibody titers. After 15 BT the recipients either had no detectable antibodies, or they had very low antibody titers. This suppression of response was shown to be distinct from a simple loss of antibody activity caused by lack of further antigenic challenge. In multiple transfused rats, humoral nonreactivity persisted in spite of rechallenge with antigen; in animals that lost their antibodies as a result of lack of further stimulation, an additional BT boosted strong antibody production. In LEW recipients of multiple BN transfusions, not only the specific anti-BN response but also reactivity to third-party BUF blood was suppressed. However, whereas the donor-specific response (anti-BN) was largely inhibited after a ten-week interval, the response to third-party BUF blood recovered. The state of humoral nonreactivity could be transferred by spleen cells to nontransfused syngeneic animals. In LEW rats that received three injections of 5 X 10(7) "suppressor" spleen cells, the antibody response to BN blood was strongly impaired as compared with animals that received normal spleen cells. BN or (BN X LEW)F1 skin grafts survived significantly better in multiple transfused LEW rats than in nontransfused controls. This was even more pronounced when ALS was given additionally. Third-party grafts (BUF) survived only slightly better than controls. It is concluded that multiple BT (1) result in humoral anti-donor nonreactivity secondary to an initial antibody response, (2) induce strong specific and weak nonspecific suppressor cell activity, and (3) increase skin graft survival.

Animals↗
PubMed1983

[Simplified methods for the measurement of renal clearance. Comparison of the results of Cr51-EDTA slope clearance, iodohippurate clearance and endogenous creatinine clearance].

Endogenous creatinine clearance proved to be unreliable in comparison to the 51Cr-EDTA-slope clearance with compartment analysis (examination time 4 hours). Tauxe's method of estimation of GFR can be recommended because of nearly perfect correlation with the 51Cr-EDTA-slope clearance (r = 0.959, n = 169, p less than 0.001). Estimation of ERPF according to Tauxe showed a moderate good correlation (r = 0.843, n = 140, p less than 0.001) with Oberhausen's method of clearance calculation (shoulder detector technique).

Chromium Radioisotopes↗
PubMed1982

Proliferation of T gamma-lymphocytes in two patients: clinical features and functional properties of the proliferating cells.

Two patients suffering form proliferation of T gamma cells exhibited uncommon clinical features, such as activation of intravascular coagulation after low dose irradiation of the enlarged spleen in one patient and isolated neutropenia in the other patients. While the malignant nature of the disease was doubtless in one patient, cell proliferation in the other patient was more likely reactive. In addition to T cell determinants the proliferating cells expressed a monocytic antigen. They did not suppress B-lymphocyte differentiation into plasma cells. In contrast the proliferating cell, especially in one patient, acted as potent effectors in NK and ADCC using melanoma and MOLT 4 target cells. Erythrophagocytosis by T gamma cells was seen in one patient. The data suggest that subsets of T gamma cells are related to the monocytic lineage and that these cells cen mediate both NKA and ADCC and partly can develop phagocytic activity.

Aged↗
PubMed1976

The morphological and clinical course of the different forms of glomerulonephritis.

From repeat biopsies of 291 patients with glomerulonephritis, the clinical and morphological course of the individual forms of glomerulonephritis were compared with one another. From these results we concluded that: 1. Every form of glomerulonephritis has in all probability its individual course and prognosis, which can only be very slightly influenced by therapy. 2. The prognosis is considerably worsened, when either hypertension develops or tubular lesions in the morphological sense of an acute renal failure.

Acute Disease↗