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D Ruano-Gil

Publications and source records attributed to D Ruano-Gil.

At least 19 recordsLinked to original sources
PubMed1998

Sciatic and femoral nerve sensory neurones occupy different regions of the L4 dorsal root ganglion in the adult rat.

The topographical distribution of sciatic and femoral nerve sensory neuronal somata in the L4 dorsal root ganglion of the adult rat was mapped after retrograde tracing with one or two of the dyes Fast Blue, Fluoro-Gold, or Diamidino Yellow. The tracers were applied to the proximal transected end of either nerve alone, or from both nerves in the same animal using separate tracers. Three-dimensional reconstructions of the distribution of labelled neurones were made from serial sections of the L4 dorsal root ganglion which is the only ganglion that these two nerves share. The results showed that with little overlap, femoral nerve neurones distribute dorsally and rostrally whereas sciatic nerve neurones distribute medially and ventrally. This finding indicates the existence of a somatotopical organisation for the representation of different peripheral nerves in dorsal root ganglia of adult animals.

Animals↗
PubMed1994

Increased cardiac endocrine activity after common bile duct ligation in the rabbit. Atrial endocrine cells in obstructive jaundice.

OBJECTIVE: This study investigated the pathogenesis of water and sodium metabolism derangements in obstructive jaundice. SUMMARY BACKGROUND DATA: Obstructive jaundice is associated with hypodipsia, depletion of extracellular water, alterations of the water and sodium regulating hormones, and an increased incidence of renal failure. Plasma atrial natriuretic factor (ANF) increases after common bile duct ligation in the rabbit. The present study was designed to investigate ANF-secreting cardiac atrial cells in this animal model. METHODS: Plasma ANF and the percentage of atrial cells staining for ANF were determined in jaundiced and sham-operated rabbits at 24 (group OJ-24, n = 11; group SO-24, n = 5) and 72 hours (group OJ-72, n = 11; group SO-72, n = 5) after surgery. The atrial ANF content was also determined. RESULTS: Plasma ANF was higher in jaundiced animals than in controls both at 24 (63 +/- 44 fmol/mL vs. 17 +/- 10 fmol/mL, p < 0.02) and at 72 hours (73 +/- 49 fmol/mL vs. 12 +/- 11 fmol/mL). In the two OJ groups, the percentage of positive ANF cells per 200-power field in the right atrial appendage was higher than in the SO groups both at 24 (62 +/- 11% vs. 31 +/- 12%, p < 0.003) and at 72 hours (56 +/- 18% vs. 31 +/- 12%, p < 0.01). Similar results were obtained in the right auricular wall. The percentage of positive ANF cells was significantly higher in the left atrium in which significant differences between the OJ and SO groups were also noted. The right atrial ANF content was higher in the OJ than in SO groups (437 +/- 323 pmol/mg of protein vs. 83 +/- 44 pmol/mg of protein). CONCLUSIONS: Cardiac endocrine activity is increased in experimental obstructive jaundice. ANF may be involved in the pathogenesis of the renal and water and sodium metabolic disturbances present in this disease.

Animals↗
PubMed1992

Effect of ascitic liquid on growth in vitro of embryoid bodies derived from teratocarcinoma.

Embryoid bodies (EB) derived from teratocarcinoma (TC) OTT6050 were cultured with ascitic liquids (AL) from animals carrying 16-, 22- and 35-day evolved EB. At the same time the presence of fibronectin (FN) in AL were analyzed by immunoblotting. Results indicate the probable existence of growth-stimulatory factors for EB, as well as the presence of FN in the 22-day AL.

Animals↗
PubMed1989

The sclerocorneal trabecula of the primates.

In this work, after having studied the chamber angle of 14 types of primates, we describe the sclerocorneal trabecula. They are a formation dependent on the tendinous insertion fibres of the longitudinal portion of the ciliary muscle. These trabecula consist of a reticulate area and another spongy area with some interstices that we think get bigger or smaller in size according to the degree of contraction of the ciliary muscle, thus either easing or impeding the flow of aqueous humour to Schlemm's canal.

Animals↗
PubMed1988

Scanning electron microscope study of the communications between the anterior chamber and the sinus venosus sclerae in primates.

We have studied the morphology of the anatomical structures that permit communication between the anterior chamber and the sinus venosus sclerae. Examination of the posterior or inner wall of this canal, represented by the sclerocorneal trabecula, in 15 species of primates and 5 adult humans, has enabled us to observe the existence of some small orifices or stomata that are the outermost part of the so-called Sondermann's canals, which in our opinion are made by the successive confluence of the interstices worked in the interior of the sclerocorneal trabecula by means of contraction of the longitudinal portion of the ciliary muscle.

Alouatta↗
PubMed1988

Recording of chick embryo movements and their correlation with joint development.

Chick embryo movements were picked up, by means of a vibration-recording technique, from the 5th day of incubation until the moment of hatching, and correlated with histological observations of the main joints. A close relationship could be demonstrated between the dates on which the first movements can be recorded and the commencement of joint formation.

Animals↗
PubMed1988

The facial motor nucleus of the dog. I. Cytoarchitectonic subdivisions and cytology.

A study of the facial motor nucleus of the dog by means of cytoarchitectonic and computer-aided three-dimensional reconstruction methods has been made. We identified three regions in this structure: lateral, intermediate and medial. Taking into account the different patterns of neuron aggregation, the following subdivisions were noted in the first, the ventrolateral and dorsolateral subnuclei; in the second, the intermediate subnucleus, and in the third, the ventromedial, intermediate-medial and dorsomedial subnuclei. Their cytological characteristics are described.

Animals↗
PubMed1988

The facial motor nucleus of the dog. II. Morphometric analysis.

In this study, an area of 953.2 micron 2, a diameter of 36.7 micron and a circularity factor of 0.74 have been established for the neurons of the facial motor nucleus of the dog. Significant differences (p less than 0.01) were observed by comparing the means of some of the parameters determined in the six cytoarchitectonic subdivisions of the facial motor nucleus described in a previous study. Moreover, the neurons tend to increase in size in the caudocranial direction.

Animals↗
PubMed1988

[Comparative study of the medial longitudinal striae of the hippocampus (formerly called "nerves of Lancisi") in the fighting bull and domestic bull].

In this paper a comparative study is made of stria longitudinalis medialis in the fighting bull and domestic bull. This nervous bundles are more developed in the former, and their connections with the Gyrus dentatus and Hippocampus are demonstrated. The structural differences observed between both animals could be related with the mechanisms that control the aggressive behaviour.

Animals↗
PubMed1988

Cytodifferentiation of mouse visceral endoderm: study with a teratocarcinoma antiserum.

In this work we separated homogeneous embryoid bodies (EB); this population is composed of only endodermal vesicles of a visceral type. These EB were used to immunize rabbits. After absorption, the antisera were analyzed immunohistochemically in sections of 7.5- and 9-day-old mouse embryos, which showed a selective reaction to visceral endoderm. The cytodifferentiation of the visceral endoderm was studied.

Animals↗
PubMed1987

Morphological significance of the pectineal ligament of the eye.

We have studied the arrangement of the pectineal ligament or its equivalent, the uveal trabecula, in herbivores, carnivores, primates and humans. From our investigations, the pectineal ligament, the uveal trabecula and the so-called processes of the iris form a morphological unit that is made up of the tendinous fibres of the longitudinal portion of the ciliary muscle, that are inserted into the periphery of Descemet's membrane and send out ahead prolongations that extend to the anterior face of the iris. The so-called processes of the iris cannot be considered as independent structures since they represent the innermost fibres of the trabecular or uveal meshwork; in some species these have a thicker appearance, an arrangement that can occasionally be found in the human eye.

Acinonyx↗
PubMed1986

Arrangement of the sclerocorneal trabecular system in human fetuses.

By studying the eyes of 20 human fetuses 33-326 mm in length (8 to 36 weeks gestation) with the light and scanning electron microscope we show that the sclerocorneal and the uveal trabecula cannot be considered as independent structures, since embryologically and morphologically they are the sclero-corneo-iridal insertions of the longitudinal fibres of the ciliary muscle.

Cornea↗
PubMed1985

Embryonal hypermobility and articular development.

This investigation was devised to correlate development of the articular system of the embryo with variations of motility by introducing reserpine to chick embryos at a Hamburger-Hamilton stage 25-26. Administration of reserpine was followed by hypermotility or paralysis in a dose-dependent fashion. Size of joint cavities varies with motility of the limbs. Larger than normal cavities are due to excess movements, while lack of these impedes differentiation. These data are consistent with the primary role of motility in articular development.

Animals↗
PubMed1984

Atypical cristae (paracrystalline inclusions) in mitochondria of epithelial cells of the rat ureter. I. Ultrastructural features.

The cells of the basal layer of the ureter epithelium of senile rat have been studied by means of thin section electron microscopy. One of the main features of these cells was the presence of paracrystalline structures within unusually long mitochondria (megamitochondria). The detailed study of these structures showed that they were produced by the mitochondrion itself through alterations of one or two longitudinally oriented cristae. The megamitochondria were completely surrounded by membranes of rough endoplasmic reticulum, frequently showed lipid lamellae at one end, and were always situated in the vicinity of the nucleus. The possible morphogenetic mechanisms of the paracrystalline structures are discussed.

Animals↗