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D Ruyter

Publications and source records attributed to D Ruyter.

5 recordsLinked to original sources
PubMed1995

Development and mapping of ten porcine microsatellite markers.

Thirty (TG)n microsatellite clones were isolated from a pig genomic library, sequenced, and tested for their suitability to detect polymorphism on a panel of animals by means of the polymerase chain reaction. Ten of these clones were developed into suitable markers and subsequently segregation of these markers was determined in the five PiGMaP reference pedigrees. A linkage analysis was performed on these 10 microsatellites together with 365 other loci that have been typed on these reference families. Eight of the microsatellites have been mapped to eight different linkage groups that have been previously assigned to different chromosomes (chromosomes 1, 6, 7, 9, 14, 15, 17 and 18). Of the remaining two markers, one is X-linked and the other shows no linkage. The number of alleles detected by these microsatellites, in the reference pedigrees, varied from six to sixteen and the heterozygosity varied from 42 to 85% in the 26 unrelated founder animals of these reference pedigrees.

Animals↗
PubMed1993

Assignment of 20 microsatellite markers to the porcine linkage map.

Twenty-one porcine microsatellite markers were developed by screening DNA libraries and by a computer search of databases. The microsatellites were typed in a large three-generation family established by a cross between the European wild pig and a Swedish Yorkshire breed. Linkage analysis benefited from the fact that due to the divergence between the parental populations, the degree of microsatellite polymorphism was significantly higher in the F1 animals than in either of the parental populations. Parallel typing of a set of 35 restriction fragment length polymorphism, protein, and blood group markers rendered it possible to assign as many as 20 of the microsatellites to the porcine linkage map. Fourteen microsatellites were localized to a chromosome segment, whereas six constituted parts of unassigned linkage groups. Analysis of four microsatellites within genes allowed the assignment of the endoplasmic reticulum Ca2+ transport ATPase locus to chromosome 14, the assignment of the interferon-gamma and the diacylglycerol kinase loci to a new linkage group (XI), and the localization of the tumor necrosis factor beta locus close to the major histocompatibility complex (SLA) on chromosome 7 to be confirmed. Fluorescence in situ hybridization mapping of two microsatellite-containing cosmids assigned two linkage groups to chromosomes 9 and 12, respectively. In total, 27 new markers were added to the porcine linkage map, thereby almost doubling the number of markers on the map. Linkage groups are now present on 10 of 18 of the pig autosomes.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1990

The nucleotide sequence of the bovine MHC class II alpha genes: DRA, DOA, and DYA.

The nucleotide sequence of the exons 2, 3, and 4, and parts of the intervening sequences of a BoLA-DRA and -DQA gene and one other class II BoLA-A gene have been determined. The structure of the BoLA-DRA and -DQA gene was found to be very similar to that of the corresponding human HLA class II genes. An analysis of the structure of the other class II BoLA-A gene showed that this A gene was clearly very different from both the human A genes and the bovine DRA and DQA genes. The results indicate that this other type of class II A gene probably represents the class II gene that has already been identified in restriction fragment length polymorphism (RFLP) studies as BoLA-DYA. Since no clear homologue of this presumed BoLA-DYA gene was found among the human HLA class II genes, these results indicate that, at least as far as the A genes are concerned, a distinct class II gene is present in cattle.

Amino Acid Sequence↗