Biomedical subjects
Publications and source records attributed to D Rymkiewicz.
In recent years people in Poland have acquired diphtheria from eastern neighbours. It was considered important therefore to study the age specific immunity against diphtheria and to compare it with the results of seroepidemiological studies conducted in t
The results of CDC peripheral test for potency of rabies vaccines were compared to the results obtained in the NIH intracerebral test recommended by the WHO. We did not find statistically significant differences in the potency of tested rabies vaccines by CDC and NIH tests.
The level of anti-tetanus antibodies were measured in 791 lots of IMIG and 330 lots of IVIG of human normal gamma globulin. The antibody levels varied 15-80 IU/ml of IMIG lots and 5-20 IU/ml of IVIG lots. Every lot of human normal gamma globulin (IMIG or IVIG) having high anti-tetanus activity may be an acceptable alternative to specific tetanus immune globulin.
The absorbed vaccine d recommended as the booster dose contained in 0.5 ml 2 Lf diphtheria toxoid and was safety and stable, the potency was 30 JO/ml. Eight adults volunteers 25-65 years received toxoid d. The tolerance and the serological response was very good.
Two methods of the estimation of the potency rabies vaccine were used: in vivo NIH Test and in vitro Antibody Binding Test (AB-Test). Six series of vaccines were studied, they passed NIH Test. The results of AB-test were comparable with the results of NIH Test. The AB-Test could be used instead NIH Test when the vaccine must be released in short time.
Forty adults volunteers 25-65 years received 10 BU tetanus and 2 Lf diphtheria adsorbed vaccine (Td). The tolerance was very good, it was not depended on the age antitoxin level. Eleven persons have not diphtheria immunity before the vaccination. The serological response was determined by the passive hemagglutination in sera collected in 4 week after the vaccination. Only in one serum the level of diphtheria antitoxin was below 0.1 IU/ml. It is recommended than two doses of Td vaccine are necessary for anyone whose last dose of diphtheria anatoxin was given more than 10 years ago especially to persons travelling to other countries at risk for diphtheria.
The new vaccine recommended as the booster dose contains in 0.5 ml: 10 BU Te-toxoid, 2 Lf Di-toxoid, 4.5-0.5 mg Al and 0.01% merthiolate. In 7 consecutive series it was state, the vaccine was safety and stable. The potency was 40-50 JO for Te and 30-40 for Di. The last series was recommended for vaccination under clinical control.
Fifty six young adults volunteers received 10 BU tetanus and 2 Lf Diphtheria adsorbed vaccine (Td). Reactogenicity was noted in the special formulas, the serological response was determined by the passive hemagglutination in sera collected before and in 4 week after the vaccination. Twelve persons were not immunity for diphtheria before booster. The results confirmed the safety and efficacy of the polish Td vaccine.
Described the stability of potency vaccines (DTP, BCG) and immunoglobulins (human's and animal's) at storage and experimental temperatures. Thermal degradation rate and design of loss of potency in time have been determined by Arrhenius equation. Our results were similar to WHO data from preparations which have been made in another countries.
The paper presents some new informations about anti-idiotypic vaccines. It discusses the advantages and disadvantages of these preparations.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
The toxins prepared by dialysis-culture method from proteolytic (P) and nonproteolytic (NP) strains of C. botulinum were different. NP toxins from 3 strains (American, Japanese and Polish) showed a higher activation ratio, lower protein nitrogen content, and lower neutralization rate compared with P toxins. Antitoxic titers of rabbit anti-P and especially anti-NP sera were always higher when titrated with NP than with P toxins. The values of the regression coefficients in neutralization experiments were not dependent on the sera preparations, but only on the toxins. The difference between the common slope b for several sera tested against P and NP toxins was statistically significant. A difference between the passive protection of mice against P and NP toxins was observed only at very low levels of unitage. These results suggested immunological heterogeneity of B type botulinum toxin. However, for practical purposes, it is not necessary to supplement therapeutic antitoxin with a factor which neutralizes NP toxin.
Explore the source record for details and available documents.