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Biomedical subjects

D S Akhlynin

Publications and source records attributed to D S Akhlynin.

3 recordsLinked to original sources

[Microbiological database UNIQEM: problems and prospects].

The UNIQEM database, designed to accumulate microbiological data of a general nature, is currently used to store and make available information about microorganisms studied and maintained at the Institute of Microbiology, Russian Academy of Sciences. UNIQEM is composed of two related databases. The first one is a catalogue database, used to accumulate and maintain list-form information on a wide range of microorganisms. The second one is a property database, which facilitates collecting, processing, and publishing diverse data having to do with these microorganisms and their properties. The database supports the retrieval of microorganisms by specifying an arbitrary set of their properties and has the potential for eventually evolving into a computer instrument for unattended identification of microorganisms.

Classification↗

[Clusterization of halophilic and halotolerant eubacteria using whole-cell protein electrophoresis data].

Total cell proteins of the nineteen halophilic and halotolerant eubacteria isolated from marine sediments and highly mineralized formation waters of oil fields were investigated by SDS gel electrophoresis. The microorganisms studied, phenotypically identified as belonging to the genera Dietzia, Rhodococcus, Staphylococcus, Cytophaga, Brevibacterium, and Archangium, were found to form clearly distinguishable clusters (20-30% similarity at the generic level) on the dendrogram derived from electrophoretic protein patterns. Protein similarity data confirmed the heterogeneity of Rhodococcus maris and its relatedness to the genus Dietza.

Bacterial Proteins↗

[Growth of mesophilic methanotrophs at low temperatures].

The optimal growth of mesophilic methanotrophic bacteria (collection strains of the genera Methylocystis, Methylomonas, Methylosinus, and Methylobacter) occurred within temperature ranges of 31-34 degrees C and 23-25 degrees C. None of the strains studied were able to grow at 1.5 or 4 degrees C. Representatives of six methanotrophic species (strains Mcs. echinoides 2, Mm. methanica 12, Mb. bovis 89, Mcs. pyriformis 14, Mb. chroococcum 90, and Mb. vinelandii 87) could grow at 10 degrees C (with a low specific growth rate). The results obtained suggest that some mesophilic methane-oxidizing bacteria display psychrotolerant (psychrotrophic) but not psychrophilic properties. In general, the Rosso model, which describes bacterial growth rate as a function of temperature, fits well the experimental data, although, for most methanotrophs, with symmetrical approximations for optimal temperature.

Adaptation, Biological↗