Biomedical subjects

D S Bunch

Publications and source records attributed to D S Bunch.

3 recordsLinked to original sources

PubMed1994

Extension of the four-parameter logistic model for ELISA to multianalyte analysis.

The standard implementation of enzyme-linked immunosorbent assay (ELISA) for single analytes can lead to false conclusions if cross reacting compounds are present in the sample. This paper discusses the extension of the usual four-parameter logistic model for ELISA to the case of multiple cross-reacting analytes. The use of the extended model in multianalyte analysis (MELISA) is illustrated and compared with a more simplistic approach. Data on the analysis of a binary mixture of s-triazines suggests the superiority of the proposed model. This model is also suitable for other forms of immunoassay that use the four-parameter logistic curve.

Cross Reactions↗

PubMed1990

Statistical design of ELISA protocols.

This paper shows how to obtain accuracy and efficiency in an ELISA analysis by allocating the wells on a 96-well microplate between calibration and determination of unknowns, and by choosing the known concentrations for calibration. The method also can determine how much is lost in precision by using a convenient but non-optimal protocol.

Enzyme-Linked Immunosorbent Assay↗

PubMed1989

When are additive models valid for evaluating proposed research?

This paper is concerned with a comparison of two broad classes of mathematical models, additive and multiplicative, for selecting the best among a number of competing project proposals addressed to a defined research objective. The analysis is predicated on the view that, though additive models are widely utilized, they are theoretically inferior to multiplicative ones. In an effort to more clearly understand when the use of additive models might be acceptable despite their logical flaws, conditions are investigated under which they yield results that are essentially equivalent to those produced by multiplicative models.

Cost-Benefit Analysis↗