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Biomedical subjects

D S Fredrickson

Publications and source records attributed to D S Fredrickson.

At least 91 records · Page 5Linked to original sources
PubMed1971

On thelipoprotein abnormality in type 3 hyperlipoproteinemia.

Two lipoprotein species were isolated by starch block electrophoresis from the very low density lipoproteins (VLDL) (S(f) 20-400) of patients with type III hyperlipoproteinemia. One of these, alpha(2)-VLDL, had a content of lipid and protein and physical characteristics similar to VLDL from normal subjects or patients with other forms of hyperglyceridemia. The other species, beta-VLDL, contained more cholesterol and less triglyceride in relation to the protein, than normal VLDL. Only the apoprotein of low density lipoprotein was immunochemically detectable in the beta-VLDL; the proteins in the alpha(2)-VLDL reacted with antisera specific for low density lipoprotein and high density lipoprotein. The electrophoretic mobility of beta-VLDL was similar to that of low density lipoprotein and significantly less than that of alpha(2)-VLDL. Isolated beta-VLDL had a lesser mean flotation rate than alpha(2)-VLDL, but both alpha(2)- and beta-VLDL were found throughout the S(f) 20-400 flotation range.The relative quantities of alpha(2)- and beta-VLDL could be varied by changing the diet or by heparin administration. Most of the VLDL from type III patients on a high carbohydrate diet was in the alpha(2)-VLDL form. During fasting, alpha(2)-VLDL fell and beta-VLDL increased becoming the predominant species of VLDL. Heparin-induced acceleration of triglyceride clearance also increased beta-VLDL and decreased alpha(2)-VLDL. These findings suggest a precursor-product relationship between the alpha(2)- and beta-forms of VLDL.

Adult↗
PubMed1971

The spleen in type I hyperlipoproteinemia. Histochemical, biochemical, microfluorometric and electron microscopic observations.

Histochemical, biochemical, microfluorometric and electron microscopic studies were made of the spleen of a patient with type I hyperlipoproteinemia. Foam cells were observed that contained a material identified as ceroid on the basis of its autofluorescence, acid-fastness, sudanophilia, PAS-positivity and insolubility in organic solvents. Electron microscopy showed that the ceroid was organized in the form of granules with concentric lamellae of irregular periodicity. The process of formation of these granules is described in detail. The ceroid was considered to represent nondigestible end products of the metabolism of chylomicrons taken up by macrophages in splenic sinusoids.

Adult↗
PubMed1970

Classification of hyperlipidaemias and hyperlipoproteinaemias.

Many studies of atherosclerosis have indicated hyperlipidaemia as a predisposing factor to vascular disease. The relationship holds even for mild degrees of hyperlipidaemia, a fact that underlines the importance of this category of disorders. Both primary and secondary hyperlipidaemias represent such a variety of abnormalities that an internationally acceptable provisional classification is highly desirable in order to facilitate communication between scientists with different backgrounds.The present memorandum presents such a classification; it briefly describes the criteria for diagnosis of the main types of hyperlipidaemia as well as the methods of their determination. Because lipoproteins offer more information than analysis of plasma lipids (most of the plasma lipids being bound to various proteins), the classification is based on lipoprotein analyses by electrophoresis and ultracentrifugation. Simpler methods, however, such as the observation of plasma and measurements of cholesterol and triglycerides, are used to the fullest possible extent in determining the lipoprotein patterns.

Age Factors↗
PubMed1970

Glycolipids and other lipid constituents of normal human liver.

An analysis of the lipids in normal human liver is presented which is particularly designed to assist in the classification and study of lipid-storage diseases. Special emphasis has been given to a determination of the quantity and composition of the neutral glycolipid classes and predominant ganglioside (Gm(3)). The neutral glycolipid content of 0.19 (sd +/- 0.11) micro moles per g wet tissue, represented 0.4% of the total lipid in liver. Ceramide dihexoside was the most abundant neutral glycolipid. The mean contents of cholesterol, glycerides, and total phospholipids were 3.9, 19.5, and 25.1 mg/g wet weight, respectively. The relative amounts of seven different phospholipid classes were also determined; these included cardiolipin (diphosphatidylglycerol), which constituted 3.9% of the liver phospholipids.

Adolescent↗