Biomedical subjects
D S Harrison
Publications and source records attributed to D S Harrison.
Family dynamics and caregiver burden in home health care.
Planning an intervention involves the assessment of medical needs followed by an analysis of the family composition, dynamics, role allocations, coping mechanisms, mode of emotional expression, and the point in the family's developmental sequence. Therapeutic targets should be identified and prioritized. At that point, a behavioral probe or some specific recommendation that the family can easily follow should be made. A careful analysis of the response will indicate the ease with which more difficult change prescriptions can be introduced. Failure of the family to follow even the most simple suggestion may indicate that family therapy is needed. If the family is successful at carrying out the probe suggestion, however, the home health care team can proceed with greater confidence that their efforts will lead to improved patient care and healthy family functioning.
The nephrotoxicity of chemotherapeutic agents.
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Human neutrophil granule heterogeneity: immunolocalization studies using cryofixed, dried and embedded specimens.
The heterogeneity in human neutrophil granules was examined by the ultrastructural localization of a series of antigens which have been previously identified with neutrophil granules by either physical separation or biochemical/biological techniques. All samples were prepared by cryofixation and molecular distillation drying (LifeCell Process), a two-step physical method that achieves cryofixation by metal mirror freezing and drying by the controlled, incremental heating of cryofixed samples in an ultrahigh vacuum. After drying, the samples were either exposed to vapor-phase osmium followed by embedment in Spurr resin, or they were exposed to formaldehyde vapor followed by embedment in Araldite resin. An indirect streptavidincolloidal gold procedure was used for immunoelectron microscopy on ultrathin sections. Subcellular ultrastructural morphology of neutrophils prepared by this method was good compared to standard electron microscopic techniques and superior compared to comparable, published electron microscopic cryomethods applied to neutrophils. Immunogold localization of myeloperoxidase, cathepsin G, lysozyme, lactoferrin, beta 2-microglobulin, and CD-15 antigens showed high intensity and specificity of labeling in the intracellular granules. Patterns of labeling varied from antigen to antigen, demonstrating granule heterogeneity both within and among neutrophils. This methodology is useful in the exploration and definition of granule heterogeneity and function.
A new technique for removal of amorphous phase tissue water without ice crystal damage: a preparative method for ultrastructural analysis and immunoelectron microscopy.
An apparatus has been produced that can remove amorphous phase tissue water via molecular distillation without devitrification or rehydration. This method represents a fundamental advance in tissue preparation, making possible for the first time ultrastructural localization of soluble molecular entities without the problems of alteration, re-distribution, and loss which have plagued conventional techniques. Fresh slices of rat brain, liver, or kidney, and monkey retinal tissue were cryofixed by bounce-free, metal mirror cooling on copper bars immersed in liquid nitrogen (LN2). Tissue transferred under LN2 was then placed in a precooled copper specimen block, which was subsequently lowered into a LN2-cooled stainless steel chamber. After rough pumping at 1 X 10(-3) mbar with a mechanical pump to remove LN2, the chamber was evacuated with a cryopump or turbomolecular pump to achieve a hydrocarbon-free, ultra-high vacuum of 1 X 10(-8) mbar. Equilibrium temperature in the chamber before the drying cycle was -192 degrees C. The copper specimen block was equipped with a thermocouple and a programmable feedback-controlled heating circuit. Tissue was dried by increasing the specimen block temperature 1 degree C/hr during the critical drying phase while monitoring the rate of water removal with a partial pressure analyzer. Results obtained indicate that drying is complete below the devitrification temperature of amorphous phase tissue water. Dried tissue was fixed with osmium tetroxide vapor, vacuum-embedded in a low-viscosity epoxy resin, sectioned, stained, and viewed with the electron microscope. Processed tissue exhibits excellent morphological preservation without the use of pre-fixation or cryoprotective agents. Thin sections of this tissue are excellent for immunocytochemical staining and electron microprobe analysis.
The impact of immigration on a depressed labour market: the South Australian experience.
"This paper examines the impact of immigration on the relatively depressed labour market in South Australia during the 1976-81 inter-censal period. The findings indicate that recently arrived immigrants have experienced exceedingly high unemployment rates. However, this is consistent with the view that immigration generates additional job opportunities for Australian residents (except in the manufacturing industry and trades occupations), and that immigrants experience high unemployment rates specifically because they have been unable to compete a 'fair share' of jobs away from residents already entrenched in the labour market."
Frequency of ovarian follicular cysts, reasons for culling, and fertility in Holstein-Friesian cows given gonadotropin-releasing hormone at two weeks after parturition.
Holstein-Friesian cows (n=204) were given saline solution or 200 microgram of gonadotropin-releasing hormone (GnRH) by intramuscular injection at 8 to 23 days after parturition. Of cows culled, fewer GnRH-treated cows were culled for infertility, compared with number of cows given saline solution (26 vs 57%; P less than 0.05). Frequency of ovarian follicular cysts was reduced from 15.2% in controls to 5.7% for cows given GnRH (P less than 0.01). The interval to 1st insemination, interval to conception, and inseminations per conception did not differ among saline solution or GnRH-treated cows which remained in the herds. These data provide evidence for reduction in infertility and reproductive disorders in early postpartum dairy cows given GnRH as a prophylactic.
Ovulation, estrus and endocrine response after GnRH in early postpartum cows.
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