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Biomedical subjects

D S Holmes

Publications and source records attributed to D S Holmes.

At least 55 records · Page 3Linked to original sources
PubMed1984

Cloning of a Thiobacillus ferrooxidans plasmid in Escherichia coli.

Three separate plasmids of 6, 7, 16, and greater than 23 kilobases were purified from a single clone of Thiobacillus ferrooxidans ATCC 33020 grown in the presence of uranium. The 6.7-kilobase plasmid (pTf1) was cloned separately into the HindIII or BamHI site of Escherichia coli plasmid pBR322. Restriction maps of the recombinant plasmids, termed pTf100 and pTf110, respectively, were constructed, creating potential cloning vehicles for exchanging genetic information between E. coli and T. ferrooxidans. Evidence from restriction enzyme analysis and Southern blot DNA-DNA hybridization indicates that the three native plasmids share little sequence homology.

Chromosome Mapping↗
PubMed1983

Effectiveness of biofeedback for treating migraine and tension headaches: a review of the evidence.

A review of the research reveals no evidence that finger temperature biofeedback training or temporal artery pulse biofeedback training is effective for treating migraine headaches. On the other hand, there is evidence that EMG biofeedback training is effective for treating tension headaches, but the EMG biofeedback training is not more effective than less complex and less expensive relaxation training. Reasons for the ineffectiveness of biofeedback training are discussed. The results of the controlled investigations are in sharp contrast to the prevailing beliefs and the results have a variety of implications.

Alpha Rhythm↗
PubMed1983

Effect of Transcendental Meditation versus resting on physiological and subjective arousal.

On four successive days, 10 highly trained and experienced meditators were asked to relax for 5 minutes, meditate for 20 minutes, and then relax for 5 minutes. In contrast, 10 other subjects who had no training or experience with meditation were asked to relax for 5 minutes, rest for 20 minutes, and then relax for 5 minutes. Physiological arousal (heart rate, skin resistance, respiration rate, systolic blood pressure, diastolic blood pressure) and subjective arousal (cognitive, somatic, relaxation) were measured throughout the experiment. Results indicated that (a) prior to meditating or resting, meditators tended to have higher heart rates and diastolic blood pressure than did nonmeditators, (b) meditation was associated with generally reduced arousal, but (c) while meditating, meditators did not evidence lower levels of arousal than nonmeditators did while resting. This investigation employed controls, which were not used in previous investigations, and the results place qualifications on previously reported results. The results have implications for the study of personality functioning, stress management, and psychotherapy.

Adult↗
PubMed1981

Influence of a "friendly visitor" program on the cognitive functioning and morale of elderly persons.

Forty-nine nursing home residents were randomly assigned to a visitation condition focusing on conversational interaction, a visitation condition in which the playing of cognitively challenging games supplemented conversation, or a no-treatment control condition. Each subject in a visitation condition was visited by an undergraduate student twice per week for 8 weeks. Before and after the visitation period, all subjects were given four tests of cognitive functioning (vocabulary, matrices, memory, problem-solving), three tests of morale (Life Satisfaction Index A; Philadelphia Geriatric Center Morale Scale, self-perceived health), and were rated by nursing home activity directors on morale, program participation, alertness, sociability, and physical condition. A multivariate analysis of covariance, in which age, education, and length of nursing home residency were covariates, revealed a reliable overall effect for the treatment (p = .001). Subjects in both visitation conditions generally demonstrated improved performance relative to control subjects, and subjects in the conversation-plus-games condition demonstrated the greatest improvement. The univariate effects for memory, self-perceived health, and ratings of sociability were reliable.

Aged↗
PubMed1980

Effectiveness of EMG biofeedback training for controlling arousal in subsequent stressful situations.

Thirty-five subjects participated in (1) a pretreatment session during which arousal was measured while subjects anticipated and then viewed a stressful film; (2) four 20-min treatment sessions during which subjects received either contingent EMG biofeedback (biofeedback treatment), instructions to attend to a variable pitch tone (attention-placebo control), instructions to relax as much as possible (instructions-only control), or instructions to sit quietly (no-treatment control); and (3) a posttreatment session that was identical to the pretreatment session. Results indicate that when compared to the subjects in the control conditions, subjects who received EMG biofeedback were not effective in reducing frontalis EMG levels during treatment or while viewing the stressful film, but they were effective in reducing frontalis EMG levels while anticipating the stressful film. There was no evidence that EMG biofeedback influenced either skin conductance or self-reports of arousal.

Arousal↗
PubMed1980

Structure of the chromosomal material in inactive nuclei of chicken red blood cells.

Electron microscope sections have been cut of chicken red blood cell nuclei by a novel procedure that produces zones of material below 100 A in thickness. After fixing and staining, electron dense structures are observed that have dimensions closely correlating with those determined for nucleosomes. These structures appear as wedge-shaped or as circular objects with a diameter of approximately 90-100 A. Five to eight of these objects are arranged in a spiral around a central core giving rise to a 250-300 A structure which may represent a superbead or a cross section of a solenoidal fibre.

Animals↗
PubMed1980

Behavior control over aversive events: does control that requires effort reduce anxiety and physiological arousal?

Although it has been suggested that the ability to control (avoid) an aversive event will reduce arousal, it may be that the effort associated with exercising the control will offset the arousal reduction associated with avoidance. To determine whether the amount of effort required to control an aversive event influenced the amount of anxiety and physiological arousal associated with the aversive event, 89 subjects participated in a 3 (unavoidable threat, avoidable threat, no threat) X 2 (high-effort task performance, low-effort task performance) X 2 (anticipation period, performance period) factorial experiment. The results indicated that (a) the aversive event (threat of electrical shock) increased subjects' anxiety and physiological arousal; (b) exercising control was effective for decreasing subjects' anxiety to the non-threat level but only when low effort was required; (c) the prospect of control decreased subjects' physiological arousal to the no-threat level while the subjects were waiting to exercise control over the aversive event; however, (d) while actually exercising the control, subjects showed high physiological arousal like that of subjects who could not control the event. These findings impose important qualifications on the speculations concerning the influence of control.

Anxiety↗