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D S KELLOGG

Publications and source records attributed to D S KELLOGG.

7 recordsLinked to original sources
PubMed1965

NEISSERIA GONORRHOEAE IDENTIFICATION IN DIRECT SMEARS BY A FLUORESCENT ANTIBODY-COUNTERSTAIN METHOD.

Direct smears from female patients have been considered unreliable for the detection of Neisseria gonorrhoeae by fluorescent-antibody (FA) methods because of inadequate background contrast of the fluorescent-stained smears and a scarcity of organisms on the smear. Evans blue dye employed as a counterstain eliminated the nonspecific background staining and increased the reliability of the direct FA procedure. Direct smears demonstrating positive fluorescence were obtained from 86% of a group of culturally positive named female contacts. The FA-counterstain technique is as sensitive as the presently recommended cultural procedures.

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PubMed1963

NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION.

Kellogg, Douglas S., Jr. (Communicable Disease Center, Atlanta, Ga.), William L. Peacock, Jr., W. E. Deacon, L. Brown, and Carl I. Pirkle. Neisseria gonorrhoeae. I. Virulence genetically linked to clonal variation. J. Bacteriol. 85:1274-1279. 1963.-One type, obtained from the purulent exudate of acute gonorrhea was maintained by 69 selective in vitro passages, at which point the organisms produced infections in human volunteers. A predominance of clonal types found in laboratory strains and a lack of ability to infect human volunteers resulted from 69 nonselective in vitro passages. Physiological and serological characteristics of the clonal types are compared. We are now in a position to study Neisseria gonorrhoeae organisms in their virulent form.

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