PubMed Health⌕ Search

Biomedical subjects

D S Parris

Publications and source records attributed to D S Parris.

25 records · Page 2Linked to original sources
PubMed1978

Regulation of herpes simplex virus type 1 DNA synthesis: temperature-shift studies with DNA-negative temperature-sensitive mutants.

The regulation of expression of viral genes involved in the synthesis of herpes simplex virus type 1 DNA was studied using three DNA- temperature-sensitive (ts) mutants (B, C, and D). These mutants were examined for their ability to synthesized viral DNA and polypeptides following temperature shift-down in the presence or absence of the transcription inhibitor actinomycin D. The results demonstrated that the B gene product is required transiently early in infection and apparently controls a transciptional step required for HSV DNA synthesis. The C gene product is required continuously during infection and also controls a transcriptional step needed for viral DNA synthesis. In contrast, the product of the D gene does not directly control a transcriptional step, is required continuously, appears to be directly involved in HSV DNA synthesis, and is probably the gene for viral DNA polymerase. The results further showed that recovery of viral DNA and polypeptide synthesis following temperature shift-down in the absence of inhibitor was greater for the D mutant than for the mutants blocked in viral DNA synthesis at the level of transcription.

DNA, Viral↗
PubMed1977

Genetics of resistance to phosphonoacetic acid in strain KOS of herpes simplex virus type 1.

A DNA- temperature-sensitive mutant of herpes simplex virus type 1 exhibiting thermolabile DNA polymerase activity, tsD9, was shown to be resistant to phosphonoacetic acid (PAA) when plated at the permissive temperature. ts+ revertants of tsD9 were PAA sensitive and exhibited DNA polymerase activity intermediate between that of the wild-type virus and tsD9, indicating that both temperature sensitivity and sensitivity to PAA are controlled by the same gene. Since the position of tsD9 on the existing herpes simplex virus type 1 linkage map is known, the locus for PAA resistance--and therefore for the structural gene for viral DNA polymerase--has been identified.

Acetates↗