Biomedical subjects

D S Passovoy

Publications and source records attributed to D S Passovoy.

3 recordsLinked to original sources

PubMed1978

Mitogens for murine embryo cell lines.

The growth-promoting activities of fetal bovine serum, cortisol, phorbol myristate acetate, prostaglandin F2alpha, insulin, epidermal growth factor, and fibroblast growth factor were evaluated on four murine embryo cell lines (Swiss 3T3, Balb 3T3, M2, and C3H10T 1/2). Each cell has an unique response spectrum to this collection of reported mitogens. Phorbol myristate acetate and prostaglandin F2alpha were active only on selected cell lines; cortisol was inactive on all four lines. Serum, epidermal growth factor, and fibroblast growth factor were able to stimulate cell division in all four lines, albeit to varying degrees for the different target cells.

Animals↗

PubMed1978

Mitogenic response to epidermal growth factor: relationship to number, affinity, and down-regulation of EGF receptors in three murine embryo cell lines.

Swiss 3T3 and C3H-M2 cells have a greater mitogenic response to epidermal growth factor (EGF) than do C3H10T 1/2 cells. The latter cell line, however, has a number of EGF receptors per cell intermediate between the two cell lines that have a more vigorous response to EGF. Scatchard analysis of binding data indicate that all three cell lines have one class of EGF receptor, with indistinguishable affinity for the ligand. When exposed to 10-nM EGF all three cell lines "down-regulate" their EGF receptors with the same time course, and to the same percentage of initial receptors.

Cell Line↗

PubMed1976

Epidermal growth factor enhancement of skin tumor induction in mice.

Subcutaneous injection of epidermal growth factor 1. significantly shortened the latency period for the appearance of methylcholanthrene induced skin tumors and 2. increased the average number of papillomas elicited per mouse in both the Swiss Webster and C3HeB/FeJ strains.

Animals↗