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Biomedical subjects

D S Zhao

Publications and source records attributed to D S Zhao.

5 recordsLinked to original sources
PubMed1998

Double enzyme-catalyzed microreactors using capillary electrophoresis.

This work evaluates the concept of a double enzyme-catalyzed microreactor using capillary electrophoresis (CE). Migrating in a capillary under electrophoresis conditions, plugs of substrate and two enzymes are injected separately in buffer and allowed to react. Extent of reaction and product ratios were subsequently determined by CE. This concept is demonstrated using two model systems: the conversion of adenosine triphosphate (ATP) to adenosine diphosphate (ADP) and adenosine monophosphate (AMP) by hexokinase (HK, EC 2.7.1.1) and apyrase (APY, EC 3.6.1.5), respectively, in the conversion of glucose to glucose-6-phosphate and inorganic phosphate, respectively, and the conversion of nicotinamide adenine dinucleotide, reduced form (NADH), to nicotinamide adenine dinucleotide (NAD) and back to NADH by lactate dehydrogenase (LDH, EC 1.1.1.27) and glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49), respectively, in the conversion of pyruvate to lactate and glucose-6-phosphate (glc-6-P) to 6-phosphogluconate, respectively. These procedures illustrate the use of the capillary as a double microreactor and the ease of quantitation of reaction products under conditions of electrophoresis.

Adenine Nucleotides↗
PubMed1998

Replantation of multi-level hand severances.

Since 1990, the replantation of hands severed at multiple levels has been carried out successfully for 22 patients. In each case the motor and sensory functions of the hand were recovered satisfactorily. We suggest that such wounds should be named 'multi-level severances' and should be classified into five groups according to the wound localities. In order to shorten ischaemic time, debridement should be carried out simultaneously by several groups. The main technical points of the operation are: (1) The order of replantation for multiply severed fingers is that the most distant section should be reattached first, and then the more proximal part. (2) High quality blood vessel anastomosis must be ensured. (3) Avoid taking the blood vessels, nerves and tendons from severed region. (4) During the operation, care should be taken to ensure that the replanted fingers' segments are protected from collision and stretching. In the early stages following the operation correct functional exercise is very important.

Accidents, Occupational↗
PubMed1994

[7 elements in patients with heart diseases and their relationship with blood pressure and biochemical target].

The trace elements Zn, Cu, Fe, and ordinary elements K, Na, Ca, Mg in serum of 711 patients with heart disease (HD) were determined with GGX-II atomic absorption spectrophotometer and flame photometer. It was found that the contents of K, Ca/Mg in HD were significantly lower than those in the control, while the content of Na, Mg, Zn, Fe, and Zn/Cu, Na/K were significantly higher than those in the control. There was a significantly negative correlation between K, Ca/Mg and blood pressure (BP), between Zn/Cu and HDL-ch, and a significantly positive correlation between Na, Zn, Fe, Zn/Cu, Na/K and BP, between Fe and Hct, between Zn/Cu and Hct, TG, VLDL-ch.

Adult↗