Biomedical subjects

D Salvatierra

Publications and source records attributed to D Salvatierra.

11 recordsLinked to original sources

PubMed1999

Cartilage and serum levels of nitric oxide in patients with hip osteoarthritis.

OBJECTIVE: To assess whether nitric oxide (NO) is related to cartilage deterioration resulting from osteoarthritis, NO concentrations were analyzed in normal and deteriorated areas of cartilage obtained from femur heads of patients with primary hip osteoarthritis (HOA). METHODS: The concentration of NO in macroscopically deteriorated and non-deteriorated cartilage of femoral heads of patients with HOA at hip replacement surgery was analyzed spectrophotometrically. Serum NO levels were also determined in 16 ambulatory patients with hip OA and in healthy volunteers. RESULTS: NO levels of non-deteriorated areas of femoral head cartilage were significantly lower (3.82+/-1.30 micromol/l; mean +/- SD) than levels of deteriorated cartilage areas (11.07+/-6.48 micromol/l; p<0.01). The surgery HOA group showed serum NO levels (2.64+/-0.32 micromol/l; p<0.0001 vs. healthy group) similar to the ambulatory HOA group levels (2.56+/-0.56 micromol/l; p<0.0001 vs. healthy group). Serum NO concentrations in healthy volunteers were 1.37+/-0.55 micromol/l. CONCLUSION: This study shows increased NO levels in joint cartilage of patients with hip OA. This increase was not homogeneously distributed, but the higher NO levels were found in macroscopically deteriorated areas. The data also suggest that high NO serum levels found in patients with hip OA may be due to joint cartilage destruction.

Aged↗

PubMed1995

Effect of acute alcohol ingestion on mineral metabolism and osteoblastic function.

Alcohol abuse can induce osteopenia in some subjects. In order to study the effect of a single dose of alcohol on mineral metabolism and osteoblastic function, we have measured calcium, phosphate, parathyroid hormone midmolecule (PTHm), parathyroid hormone intact molecule (PTHi) and bone-gla-protein (BGP) in serum of 8 healthy men after the ingestion of a single dose of alcohol (0.6 g/kg body weight). Urinary calcium and magnesium were also measured. After alcohol intake, both serum PTHm and PTHi were decreased, as well as serum BGP. Serum phosphate and urinary calcium and magnesium were increased. An inverse significant correlation was found between PTHi and serum phosphate (r = 0.42; p < 0.02). Our data show that acute alcohol ingestion lowers serum PTH and BGP in humans, suggesting an inhibitory effect on parathyroid and osteoblastic function. These changes and the alcohol-induced transient hypercalciuria could contribute to the development of bone disease associated with chronic alcohol abuse.

Adult↗

PubMed1994

Association of HLA-DR5 with mucocutaneous lesions in patients with rheumatoid arthritis receiving gold sodium thiomalate.

OBJECTIVE: To investigate the possible association between HLA antigens and adverse reactions to gold sodium thiomalate therapy (GSTM). METHODS: Ninety consecutive patients with rheumatoid arthritis (RA) were studied for possible association between HLA antigens and adverse reactions to GSTM therapy. RESULTS: HLA-DR5 was significantly increased in patients who developed gold induced mucocutaneous lesions. On the other hand, patients with RA carrying B8 and DR3 antigens are of a high risk of developing proteinuria after gold therapy. A very interesting finding was the low incidence of DR7 antigen in patients who developed adverse reactions to GSTM. We also report the relationship between B27 antigen and chrysiasis due to gold therapy. CONCLUSION: Our results support suggestions that the DR7 antigen provides a protective effect against gold toxicity. We also found a strong association between DR5 and mucocutaneous lesions in patients with RA treated with GSTM.

Adult↗

PubMed1985

HLA antigens in brucellosis.

106 patients diagnosed as having brucellosis were typed for HLA class I and class II antigens. The number of healthy controls was 272 and 102 for classes I and II, respectively. 17 HLA-A, 31 HLA-B, 7 HLA-C and 8 HLA-DR specificities were studied. The most important finding was a highly significant decrease of the HLA-Cw2 antigen frequency in the patient group compared with the controls. The corrected p value was p less than 0.0001 (Fisher's test). This finding is compatible with the idea that HLA-Cw2 antigen could be a protective factor. No other positive or negative association was observed. There was no significant difference in HLA antigen frequencies between patients with and without joint manifestations. In addition, we did not find an HLA-B27 increase in the group of patients with brucellosis associated spondylarthritis when compared with healthy individuals.

Arthritis↗

PubMed1977