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Biomedical subjects

D Sauer

Publications and source records attributed to D Sauer.

At least 37 records · Page 2Linked to original sources
PubMed1986

[Nutritional status in stomach cancer--a criterion for evaluating operability?].

The nutritional status of 41 male patients with carcinoma of the stomach was recorded, right after admission to hospital, and was related to tumour-specific operability. The nutritional condition of most of the patients was moderately impaired. Significant differences between operable and non-operable patients were recorded from the following parameters: upper-arm circumference, haemoglobin concentration, serum albumin, pseudocholinesterase, intradermal testing, E-rosette count. The findings thus obtained provided some limited information on operability.

Body Weight↗
PubMed1985

One-carbon metabolism in lectin-activated human lymphocytes.

Serine is an essential amino acid for the lectin-mediated transformation of human peripheral blood lymphocytes due to the inability of this cell to synthesize sufficient quantities via either the phosphorylated pathway or by reversal of the serine hydroxymethyltransferase reaction to meet the metabolic demands. The level of intracellular serine is tightly regulated, and the culture medium concentration for optimum cellular transformation falls within a relatively narrow range. The three-carbon atom of serine is the major source of one-carbon units required for purine and pyrimidine nucleotide biosynthesis, but the key effect of both serine deprivation and of high medium serine levels would appear to be on protein synthesis. Although an alternative source of one-carbon units, as provided by high levels of formate in the culture medium, can partially reverse the effects of serine deprivation, the only other demonstrable source of one-carbon units, tryptophan, requires serine for its incorporation and subsequent metabolism. Methionine is also essential for lymphocyte transformation and is involved in the synthesis of a small amount of phosphatidylcholine, although most of this phospholipid is provided by choline and lysophosphatidylcholine from the serum-supplemented culture medium.

Blood Proteins↗
PubMed1985

[The antigen-specific rosette test--its value for clinically relevant problems].

The antigen specific rosette test was used for the evaluation of the sensibilization in animal models and in patients to antigens with clinical relevance. The corresponding antigens were coupled to sheep- and guinea pig-erythrocytes respectively or to a synthetical carrier and we have determined the frequency of antigen binding lymphocytes in patients or animals by rosette formation. The suitability of this test we have studied for following problems: Evaluation of a sensitization against a cutaneous burn toxin in mice following thermic injury, against the transplantation antigens in pigs following allogenic renal transplantation, against TAA from breast carcinoma in patients with breast cancer.

Animals↗
PubMed1984

[Immunologic studies in patients with Crohn disease and ulcerative colitis].

To compare patients with Crohn's disease and patients with ulcerative colitis we have studied some immunological parameters (level of IgM, IgG and IgA in the serum, number of lymphocytes, E rosettes) and the immunological reactivity to antigens from colon carcinoma and to colon antigens prepared from healthy and from colon tissue altered by Crohn's disease (antigen specific rosette test, MEM-test, antibody titer). The results indicate a decreased proportion of T cells in both bowel diseases on the one hand, on the other hand a specific cell-meditated immune reaction to colon antigens could be observed in patients with Crohn's disease. In patients with ulcerative colitis such reactions are determined only in the acute phase of disease or in recidive. It remains to be elucidated whether these immunological reactions represent a basic pathogenetic factor in the onset of these diseases.

Antigens, Neoplasm↗
PubMed1983

De novo purine synthesis in human lymphocytes. Partial co-purification of the enzymes and some properties of the pathway.

A partially purified enzyme extract from lectin-transformed human peripheral blood lymphocytes synthesized purine nucleotides de novo. Although the relatively lower specific activity of the pathway compared with that in the avian liver preparation previously described (Rowe, P. B., McCairns, E., Madsen, G., Sauer, D., and Elliott, H. (1978) J. Biol. Chem. 253, 7711-7721) limited the extent of purification, a number of properties were established: (i) Ammonia could be utilized as readily as glutamine for the synthesis of phosphoribosylamine but only glutamine provided N-3 of the purine ring; (ii) in the presence of either GTP or NAD, AMP or GMP were synthesized; (iii) purine synthesis was inhibited at the level of phosphoribosylamine synthesis by both AMP and GMP, irrespective of whether ammonia or glutamine was the N donor; (iv) while the synthesis of AMP and GMP from IMP was self-regulated, GTP also appeared to be an inhibitor of the synthesis of GMP from IMP; (v) amidophosphoribosyltransferase was isolated from both transformed and nontransformed cells in a low molecular weight form which was converted to a high molecular weight form in the presence of GMP; and (vi) no evidence was obtained for the existence of a classical multienzyme complex for purine synthesis.

Adenosine Monophosphate↗
PubMed1982

[Immunotherapy of burns--animal experiments].

Immunologic changes are a component of the pathophysiology of severe burns. The effect of the burn toxin can be reduced by immunologic mechanism. The antigen elimination by immunologic defense mechanism additionally to the necrectomy was discussed. A significant therapeutic effect after standardised burn in the animal mouse model showed the application of heterologous antibodies against the burn antigen and the specific immunostimulation. The antibody titre and the number of lymphocytes which bind burn toxin were investigated. The animal experimental results show the real chances of the antigen elimination by passive immunisation respectively immunostimulation. Immunosuppressive influence by therapeutic measures must be avoided.

Animals↗
PubMed1981

[Immunological and immunotherapeutic studies on the model of mice following thermic injury ].

A burn toxin, which is discussed as reason of the burn disease by Schoenenberger et al., was isolated from burned skin of mice and the immunological reactions to this antigen were evaluated in burned mice longitudinally at several intervals after the trauma. We determined the specific antibodies against the burn toxin by means of the passive hemagglutination test, the burn toxin binding cells by means of the antigen specific rosette technique and the cell-mediated immunity using the macrophage electrophoretic mobility test (MEM-test). A rise of the burn toxin-specific cells could be observed with maximum at day 4 and a cell-mediated immunity between day 11 and day 13. Antibody titre was on the background about an interval of 3 weeks. Pretreatment with burn toxin or posttraumatic i. p. application of antibodies against burn toxin from rabbits eliminated primary death of mice with lethal burns. No correlation seemed to exist between the effect of treatment with antibodies and the antibody titre in the first days following burns.

Animals↗
PubMed1981

[Immunological investigations of patients after thermic injury (author's transl)].

A burn toxin, which is important for the manifestation of clinical and septical processes during the burn disease, was isolated from burned skin of mice. Using this antigen we have studied the immunological reactions against burn toxin in 10 patients following thermic trauma. We determined the specific antibodies against the burn toxin by means of the passive hemagglutination test, the level of IgM, IgG and IgA in the serum using Mancini-immunodiffusion, the burn toxin binding cells by means of the antigen specific rosette technique and the cell-mediate immunity using the macrophage electrophoretic mobility test (MEM-test). A rise of the burn toxin-specific cells could be observed with maximum at day 4 and a second peak at day 10 and cell mediated immunity between day 10 and day 15. The titer of specific antibodies increased, most following an initial decrease, after day 6, a correlation seemed to exist between this titer and the level of immunoglobulins in the serum.

Antibody Formation↗
PubMed1978

De novo purine synthesis in avian liver. Co-purification of the enzymes and properties of the pathway.

The enzymes of the de novo purine biosynthetic pathway have been partially co-purified from pigeon liver by a method dependent upon the use of the nonionic polymer polyethylene glycol for enzyme stabilization and cofractionation. Although the enzymes did not appear to constitute a large macromolecular complex it was evident that some particular inter-relationship between them was preserved during the purification procedure. Analysis of the end products and pathway intermediates was carried out primarily by sensitive high pressure liquid chromatographic techniques. Substrate and cofactor requirements were confirmed and optimal conditions of pH, temperature, and K+ ion activation established. At phosphoribosyl pyrophosphate (PP-ribose-P) concentrations below 0.3 mM the activity of the first pathway enzyme amidophosphoribosyltransferase was rate-limiting, and the inhibition of this enzyme by AMP regulated the rate of purine ring synthesis. At higher concentrations of PP-ribose-P, aminoimidazole ribonucleotide synthetase, the fifth enzyme of the pathway became rate limiting and was subject to inhibition by added AMP. It was evident that the regulation of purine synthesis was quite complex and that AMP inhibition (perhaps reflected in a low adenylate energy charge) can be effected at different points on the purine pathway.

Amidophosphoribosyltransferase↗
PubMed1978

[Effect of infection on the immune system in burns in animal experiments].

After discussing a model of infection in mice and the possibility of active immunization against pyocyanic pseudomonas, the authors describe the effect of a lipid-protein-complex achieved from isolated burnt mouse skin on pseudomonas infection. First results showed that sublethal amounts of that complex at otherwise less pathogenic suspension of pseudomonas cause a lethality of 100 per cent. This effect can partially inhibited by application of antitoxic IgG. The lethal course was not primarily caused by the infection but by the above mentioned complex which significantly lowers the resistance against pyocyanic pseudomonas.

Animals↗