Biomedical subjects

D Sauser

Publications and source records attributed to D Sauser.

2 recordsLinked to original sources

PubMed1976

Production of xenogeneic and allogeneic antisera specific for mouse thymus-derived lymphocytes.

Xeongeneic and allogeneic antisera were raised in rabbits and mice using purified mouse T cell-specific antigens. These antisera were shown to be reactive in complement and antibody-dependent cell-mediated cytotoxicity tests as well as in immunofluorescence, with 30% of mouse spleen cells, 80% of lymph node cells and 100% of thymocytes or various T lymphoblastoid cell lines labeled. No reaction could be detected with bone marrow cells or non-T cell lines. Both types of reagents bound to the same spleen lymphocyte subpopulations and competed for the same antigenic sites as anti-Thy-1.2 alloantiserum. These antisera raised with small amounts of immunogens did not require any absorption to be rendered specific for mouse T cells.

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PubMed1976

Studies of mouse-brain-associated T-lymphocyte antigens.

Xeno- and alloantigens shared by mouse brain, thymocytes and thymus-derived lymphocytes were isolated from brain by butanol extraction, followed by Sephadex G-200 column chromatography and preparative polyacrylamide gel electrophoresis, both in the presence of SDS. The antigenic activity, as tested by inhibition of complement-mediated cytotoxicity using a xenogeneic antiserum directed against T cell-specific antigens (anti-MTLA), was enriched 50-fold during the purification procedure. Xenogeneic and allogeneic antisera which were specific for mouse T-lymphocytes, could be obtained by immunization of rabbits and mice with the partially purified antigens.

Animals↗