[ESR study of two forms of soluble ferredoxin from pea leaves].
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Biomedical subjects
Publications and source records attributed to D Sh Burbaev.
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Interaction between methemoglobin and cyanide was studied by low temperature inhibition method in combination with ESR. A new, not earlier described in literature ESR signal of low spin cyanide complex of this protein was recorded.
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The data on the action of the iron-sulfur centre N-2 in the 1 site of respiration and oxidative phosphorylation coupling obtained by the authors as well as by other investigators are reviewed in terms of conformational - relaxation concept of energy transformation. The study of the direct and reverse electron transport bears testimony to the fact that the N-2 centre with its proper protein surrounding plays the role of the protein transformer of energy in the 1 site.
The dramatic diminishing of the concentration of the N-2 iron-sulfur centre of NADH-dehydrogenase of mitochondria during the growth of cell culture of hamster fibroblasts with the subsequent recovery of concentration to the initial level was discovered by means of low-temperature ESR spectroscopy. It was concluded that the results obtained are due mainly to the decrease of the number of respiratory chains, but not to the change of the electron transport chain structure.
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It has been shown by the method of low-temperature ESR-spectroscopy that partial blocking with formaldehyde of electron intake into the respiratory chain promotes the maintenance of a definite level of oxidation in the respiratory chain carriers and preservation of its nativity.
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The curves of temperature relationship of signal intensity (amplitude) with g = 2.02-2.03 may serve as a test for identification of iron-sulfur centres of S-3 and aconitase at different metabolic states of mitochondria in the course of their reduction with various substrates
Several types of flavins and quinones having different parameters of magnetic relaxation have been detected in the mouse liver tissue by means EPR technique. The values of these parameters depend on the redox state of the respiratory chain meal-containing centres. This means that there is an interaction between the free radicals and the metal-containing paramagnetic centres.
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