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Biomedical subjects

D Sinclair

Publications and source records attributed to D Sinclair.

35 records · Page 2Linked to original sources
PubMed1986

The incidence of monoclonal gammopathy in a population over 45 years old determined by isoelectric focusing.

Immuno-isoelectric focusing (IIEF), a technique previously shown to be sensitive for the detection of paraproteinaemia, was used to test 200 individuals over the age of 45, without history of B cell neoplasm, for the presence of serum paraproteinaemia. 11% of these individuals had evidence of paraproteinaemia detectable by IIEF compared with only 2% by zonal and immunoelectrophoresis. A further 12% had oligoclonal immunoglobulins and the remainder had no qualitative abnormality of the immunoglobulin profile. These results are discussed with particular reference to the aetiology, diagnosis and monitoring of potential B cell neoplasm in high risk individuals or groups.

Aged↗
PubMed1986

The incidence and possible relevance of Bence-Jones protein in the sera of patients with multiple myeloma.

We present results showing that the detectable incidence of Bence-Jones protein in the sera of patients with multiple myeloma is equivalent to the highest detectable incidence of Bence-Jones protein in highly concentrated urine when tested by immuno-isoelectric focusing. In a series of 25 multiple myeloma patients, immuno-isoelectric focusing showed the incidence of Bence-Jones proteinemia to be 68% compared with a detectable incidence of Bence-Jones proteinuria of 32-64% by immunoelectrophoresis and immuno-isoelectric focusing depending upon the urine concentration factor. In view of the variability of factors governing urinary Bence-Jones protein levels, such as renal catabolism, reabsorption and losses during concentration, we suggest that monitoring of serum Bence-Jones protein by immuno-isoelectric focusing should be carried out in conjunction with measurements of urinary Bence-Jones protein in multiple myeloma.

Adult↗
PubMed1985

The potential value of immuno-isoelectric focusing in the diagnosis and management of solitary plasmacytoma.

Immuno-isoelectric focusing is a highly sensitive method of detecting small quantities of monoclonal protein. We have previously reported the first demonstration of serum paraprotein in non-secretory myeloma using this technique (Sheehan et al. 1985). We now report a case of solitary plasmacytoma where this technique revealed a small concentration of serum paraprotein when conventional techniques failed, and discuss the potential value of focusing in plasma cell dyscrasia, with limited tumor cell mass. focusing in plasma cell dyscrasia, with limited tumour cell mass.

Aged↗
PubMed1985

The effect of high-dose fentanyl anaesthesia on P50 in man.

The effect of high dosage fentanyl anaesthesia on P50 was studied in patients undergoing coronary bypass surgery. After induction of fentanyl anaesthesia 100 micrograms X kg-1 with pancuronium muscle relaxation P50 changed significantly (P less than 0.005) from 3.40 +/- 0.12 to 3.33 +/- 0.13 kPa. This anaesthesia technique decreases P50 in vivo, but this has more theoretical than practical importance.

Aged↗
PubMed1978

Multiple receptors for brain dopamine.

This study was done to obtain direct in vitro evidence for the possible existence of more than one type of dopaminergic binding site in homogenates of the caudate nucleus from calf brain. Five radioligands for dopaminergic sites were tested. The inhibitions of two agonist radioligands ([3H]dopamine, [3H]apomorphine) by haloperidol, chlorpromazine, or piflutixol were biphasic. The inhibitions of [3H]haloperidol, [3H]spiroperidol, and [3H]dihydroergocryptine binding by dopamine and (-)-norepinephrine were also biphasic. Thus, both 3H-labeled agonists and 3H-labeled antagonists were possibly binding to two high-affinity sites.

Animals↗
PubMed1976

Cyclic AMP and protein kinase in the spontaneously hypertensive rat aorta and tissue-cultured aortic smooth muscle cells.

Cyclic AMP levels and protein kinase activity were determined in the aortas of rats with normotension, moderate and severe spontaneous hypertension. While the cyclic AMP levels were reduced in the aortas from rats with moderate and sever hypertension the protein kinase levels were found to be elevated only in the aortas from rats with severe hypertension. We have grown in tissue culture, aortic smooth muscle cells from the normotensive and severely hypertensive rat. Cultured cells from both strains have similar growth patterns and morphology. The differences seen in cyclic AMP and protein kinase levels in the intact aortas are also seen in the aortic smooth muscle cells in cluture.

Animals↗
PubMed1975

A novel form of diffusion battery.

A novel form of diffusion battery uses a series of 635-mesh screens instead of the customary cluster of circular tubes or holes. Fifty-five screens are arranged in series so that the amount of aerosol penetrating through different numbers of screens can be measured with a condensation nuclei counter. This "filter stack" must be calibrated because theory is not available to calculate the performance of such screens with sufficient accuracy. For calibration a polydisperse aerosol such as ambient or silver aerosol is used to compare the performance of the screens with that of the cluster-tube and collimated-holes diffusion batteries, whose performance is known from theory. It was observed that a given number of screens is equivalent to a given number of batteries independent of the size or size distribution of the aerosol. This filter stack is very compact and light in weight (0.9 kg). It is particularly useful for measurements in the field.

Aerosols↗
PubMedDate not supplied

A retrospective review of the relationship between facial fractures, head injuries, and cervical spine injuries.

Facial fractures are common in the multiply-traumatized patient, and the results of previous studies that have examined the relationship between facial fractures, cervical spine injuries, and head injuries have produced conflicting results. A retrospective review of 1,050 blunt-trauma patients during a 40-month period at a regional trauma unit revealed 168 patients with facial fractures. The average injury severity score (ISS) of these patients with facial fractures was 31 compared with an average ISS of 25.6 for the entire group. There were 266 facial fractures in these 168 patients: mandible (n = 72), maxilla (n = 74), zygoma (n = 52), orbital (n = 46), nasoethmoidal (n = 20), and frontal (n = 2). There were seven (4%) cervical spine injuries, three of which were atlanto-occipital subluxations in patients who ultimately died. Of the four surviving patients, one had a cord injury. In contrast, 145 (85%) showed evidence of head injuries; 64 (38%) of these head injuries were serious and 17 of these patients required craniotomy. The relationship of facial fractures and cervical spine injuries may be over emphasized; head injuries, although frequently minor, are much more commonly associated with facial fractures.

Adolescent↗