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Biomedical subjects

D Solter

Publications and source records attributed to D Solter.

At least 91 records · Page 5Linked to original sources
PubMed1980

Cell line derived from a metastasis of a human testicular germ cell tumor.

A cell line, designated 833K-E, has been established from a metastasis of a human testicular germ cell tumor that consisted of four histological types of tumor cells. The 833K-E cells have morphological and ultrastructural characteristics of epithelial cells and a hyperdiploid karyotype indicative of their human male origin. The cells grow in agar cultures and produce in nude mice tumors which have the hstological features of embryonal carcinoma without differentiated elements. Many of the cells express a stage-specific mouse embryonic antigen, and low levels of the major histocompatibility antigens and beta 2-microglobulin also were detected on a large percentage of the cells. A lymphoblastoid cell line (833K-LC) established from the same tumor specimen expresses major histocompatibility antigens and beta 2-microglobulin but does not express the embryonic antigen.

Animals↗
PubMed1979

Embryo-derived teratocarcinoma: I. The role of strain and gender in the control of teratocarcinogenesis.

The role of gender and genetic (strain-specific) factors in the regulation of teratocarcinogenesis was studied by monitoring the outgrowth of benign and malignant embryo-derived teratoid tumors, i.e., teratomas and teratocarconomas in several mouse strains. Teratocarcinomas were produced in all mouse strains tested, but the ratio of teratoma to teratocarcinoma varied from one strain to another. A high yield of teratocarcinomas was obtained in A/J, BALB/cJ, DBA/2J, CBA/J and C3H/J mice, irrespective of the sex of the recipient. A low yield of teratocarcinomas was obtained in both male and female C57BL/6J and AKR/J recipients, and in 129/J female recipients. For all strains but 129/J and eventually AKR/J the sex of the recipients did not significantly affect the outgrowth of embryo-derived tumors. These data suggest the existence of mouse strains with high and low permissiveness for embryo-derived teratocarcinogenesis. The sex of the recipients may influence the yield of embryo-derived teratocarcinomas in some mouse strains but is of no consequence in others.

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PubMed1979

Teratomas.

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Animals↗
PubMed1979

Cytoplasmic and nuclear protein synthesis in preimplantation mouse embryos.

Cytoplasmic and nuclear proteins synthesized by mouse embryos at different stages of preimplantation development were analyzed by two-dimensional polyacrylamide gel electrophoresis. Several nuclear-specific proteins (i.e. proteins more abundant in the nucleus than in the cytoplasm) and numerous cytoplasmic-specific proteins were observed. The trends of changes in the nuclear and cytoplasmic protein synthesis are similar. Moderate changes occur between the unfertilized egg and the zygote. Striking changes characterized by the disappearance of numerous major oocyte-specific proteins and the appearance of a large number of new, stage-specific proteins occur between the zygote and the 4- to 8-cell stages. In contrast, between the 4- and 8-cell and early blastocyst periods, only a few new proteins appear, and a small number of oocyte-specific or other stage-specific proteins disappear. Minor differences in protein synthesis were observed between the trophoblast and inner cell mass.

Animals↗
PubMed1978

Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1).

A monoclonal antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with F9 teratocarcinoma cells is described. This antibody, which reacts with embryonal carcinoma cells of mouse and human origin and with some preimplantation stage mouse embryos, defines an embryonic stage-specific antigen. This stage-specific antigen (SSEA-1) is first detected on blastomeres of 8-cell stage embryos. Trophectodermal cells are transitorily positive; however, each cell in the inner cell mass eventually expresses this antigen.

Animals↗
PubMed1978

Infection of mouse preimplantation embryos with simian virus 40 and polyoma virus.

Mouse two-cell embryos, morulae, and blastocysts were killed when infected in vitro with simian virus 40 (SV40) at high multiplicities of infection. Polyoma virus was not deleterious for preimplantation embryos, even at a very high multiplicity of infection; however, the outgrowths of polyoma-infected blastocysts disintegrated after several days of culture. Indirect immunofluorescence tests revealed the presence of SV40 T and V antigens and polyoma virus V antigen in the nuclei of trophoblastic cells. Virus-specific antigens were not found in the nuclei of cells forming inner cell masses of blastocysts or in inner cell mass-derived cells in blastocyst out-growths. The appearance of SV40 T and V antigens in the nuclei was inhibited by alphaamanitin, a RNA polymerase II inhibitor. The amount of infectious virus recovered from cultures of morulae or blastocysts on subsequent days after infection with SV40 initially declined but later increased. These points of evidence indicate that some cells of early mouse embryos are permissive for the expression of early and late functions of SV40 genome and that susceptibility to infection with polyoma virus and/or permissiveness for the expression of polyoma virus late functions develop gradually between the two-cell and blastocyst stages. Electron microscope observations showed the presence of specific complexes of membranes and virions in the cytoplasm of trophoblastic cells. Single viral particles could be found in the nuclei and also in mitochondria.

Amanitins↗
PubMed1978

Effect of removal of the zona pellucida on subsequent development of mouse blastocysts in vitro.

Removal of the zona pellucida allowed mouse blastocysts incapable of hatching in vitro to develop in culture. Blastocysts denuded by pronase always developed further than those of identical age that had hatched spontaneously. More blastocysts mechanically denuded and treated with pronase developed egg cylinder-like structures than did blastocysts mechanically denuded and not treated with pronase. Plastic support gave better development of blastocysts than did glass.

Animals↗
PubMed1977

Complement-mediated antiserum cytotoxic reactions to human chromosome 7 coded antigen(s): immunoselection of rearranged human chromosome 7 in human-mouse somatic cell hybrids.

Immunoselection via complement-dependent lysis of human-mouse somatic cell hybrids containing chromosome 7, with antisera reactive to cell surface antigen(s) coded for by chromosome 7, has resulted in growth of somatic cell hybrids containing rearranged human chromosome 7s. Investigation of these hybrids has localized the gene(s) coding for the relevant cell surface antigen(s) to the short arm of human chromosome 7. The simian virus 40 integration site and the gene coding for human beta-glucuronidase appear to be localized to the long arm of chromosome 7 in this hybrid clone.

Animals↗
PubMed1977

Ultrastructural study of concanavalin-A binding to the surface of preimplantation mouse embryos.

Receptors for Con-A were labelled (using the peroxidase-diaminobenzidine technique) on the plasma membrane of unfertilized and fertilized mouse eggs, cleavage stage embryos, trophoblast and inner cell mass (ICM) of the blastocyst. Embryos were exposed to Con-A concentrations of 10 microgram/ml, 50 microgram/ml, or 1,000 microgram/ml and the lowest concentration was observed to be the most suitable for discerning differences between stages of embryonic development. On the surface of unfertilized and fertilized eggs and 2-cell embryos, reaction product appeared as a thin, discontinuous layer. The surface of 4- and 16-cell stage embryos had a thicker, continuous, although non-uniform, layer of the reaction product. On the surface of the cells of the late morula, and on the trophoblastic cells of the blastocyst, clustering of reaction product was observed. Cells of ICM of intact blastocyst were free of the reaction product, showing that either Con-A and/or peroxidase cannot penetrate tight junctions between trophoblastic cells. Reaction product in the form of a thin, uniform layer covered the free surface of the cells of the ICM after they had been isolated (using immunosurgery) and exposed to 50 microgram/ml of Con-A. The amount and distribution of Con-A receptors is discussed, along with their redistribution and mobility in relation to the agglutinability of preimplantation mouse embryos.

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