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Biomedical subjects

D Stedman

Publications and source records attributed to D Stedman.

7 recordsLinked to original sources
PubMed1996

Importance and difficulty of determining the cost of clinical research.

The cost of clinical research has become increasingly important over the last few years because of changes in the health care market and the resulting decline in hospital margins. The emergence of contract research organizations has also caused academic medical centers to pay attention to costs. Although the consideration of clinical research costs has become vital, a number of factors make cost evaluation a difficult process. These include confusion about terminology, lack of specificity, estimation of the cost of complications, and duration of patient participation, as well as the assignment of personnel costs and infrastructure costs.

Academic Medical Centers↗
PubMed1985

Simian virus 40 large T antigen oligomers: analysis of electrophoresis in the absence of detergent.

Large T antigen of simian virus 40 is found as monomeric and oligomeric species in transformed cells. These can be demonstrated in cell extracts by velocity centrifugation in sucrose gradients. We analyzed them further in a transformed human line cell (SV80) and a transformed mouse line cell (SVT2). Individual fractions from sucrose gradients were subjected to polyacrylamide gel electrophoresis in the absence of detergent. T-antigen species were then detected by protein blotting and antibody overlay with polyclonal anti-D2 T antibody or monoclonal Pab419, Pab101, or Pb1700 antibody. The rapidly sedimenting species (14S and larger) of large T antigen from both cell lines reproducibly showed two major bands with estimated molecular weights of 670,000 and 850,000. A third band of 1,200,000 was more prominent in SVT2 cells than in SV80 cells. In SV80 cells the slowly sedimenting species of large T antigen (5S to 11S) contained two reproducible bands. A band with a molecular weight of 95,000 was the predominant one in all fractions between 5S and 11S. A relatively minor band with a molecular weight of 230,000 was found in fractions between 9S and 11S. The low-molecular-weight forms were seen in SVT2 cells only when a prominent peak at 5S to 7S was present, that is, when extracts were stored before analysis. In fresh extracts, the low-molecular-weight bands and slowly sedimenting forms were absent.

Animals↗
PubMed1981

Immunological cross-reactivity between simian virus 40 large T antigen and D2 hybrid T antigen.

A specific antiserum was raised in rabbits against D2 hybrid T antigen that had been purified from HeLa cells infected with the adenovirus/simian virus 40 hybrid, Ad2(+)D2. The specificity of this serum was compared with that of a conventional hamster antiserum against simian virus 40-induced tumors by immunoprecipitation and by a new radioimmune assay that can detect nanogram quantities of D2 hybrid T antigen.

Adenoviruses, Human↗