Biomedical subjects

D Stegwee

Publications and source records attributed to D Stegwee.

2 recordsLinked to original sources

PubMed1986

Cellular Interactions during the Mating Process in Chlamydomonas eugametos.

A method to determine the mating competence of Chlamydomonas eugametos was developed. The contribution of each mating type in the pair formation was investigated using asymmetric gamete mixtures. It was established that pair formation is not mediated by a pheromonal attraction mechanism between partner gametes, but depends on collision chances. On the other hand, it was demonstrated that during transient contacts between partner gametes the flagellar agglutinability of both partners is stimulated, evidently to prepare a successful mating. The plus mating type was generally less agglutinable than the minus mating type and was a rate-limiting factor in the mating process.

Journal Article↗

PubMed1981

Arabinogalactan Protein from a Crude Cell Organelle Fraction of Phaseolus vulgaris L.

Sonication of a crude cell organelle fraction from hypocotyl tissue of dark-grown bean seedlings, and from suspension-cultured cells released a hydroxyproline-containing protein. The purification of this protein is described. It was found to be an arabinogalactan protein composed of 90% carbohydrate and 10% protein. The major sugars are galactose, arabinose, and uronic acids, and the major amino acids are hydroxyproline, serine, and alanine. Its molecular weight was estimated at 1.4 x 10(5) daltons and the isoelectric point at pH 2.3. The molecule is soluble in 5% trichloroacetic acid and can be precipitated with beta-galactosyl Yariv antigen. Pulse-chase experiments indicated that it was a secretory protein. The biosynthesis of arabinogalactan proteins is discussed.

Journal Article↗