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Biomedical subjects

D Szalay

Publications and source records attributed to D Szalay.

7 recordsLinked to original sources

Application of main component fraction collection method for purification of compound libraries.

In order to support high-throughput library purification, a novel UV triggered fraction collection method was developed in which a maximum-seeking-algorithm-driven, six-port valve collects the largest chromatographic peak. This straightforward strategy achieves the one sample-one fraction approach, thus resulting in a simpler and less error prone workup procedure. The effectiveness of this main component fraction collection method will be illustrated here by the results of the purification of compound libraries (altogether 6086 compounds, having an averaged success rate of 79.4%). Advanced applications, where the desired component differs from the main component, will also be discussed.

Chemical Fractionation↗

Clinical signs and mortality caused by Ornithobacterium rhinotracheale in turkey flocks.

Upper respiratory tract disease (manifesting itself in rhinitis, tracheitis and conjunctivitis) and mortality associated with Ornithobacterium rhinotracheale infection were observed in four flocks of 2- to 3-week-old turkeys. In a 15- to 16-week-old turkey flock bilateral catarrhal-croupous pneumonia was found in the dead birds. In a further 5-week-old flock and in three 16- to 20-week-old turkey flocks mortality was preceded by nervous signs (motor disturbances, recumbency, abnormal carriage of the head) and was found to be associated with fibrinopurulent inflammation of the cranial bones and meningitis. The bacterium O. rhinotracheale was isolated from the affected organs in the different disease conditions. The isolated strains did not differ markedly in cultural, morphological and biochemical properties. This is the first report of a turkey disease manifesting itself in nervous signs associated with O. rhinotracheale infection.

Animals↗

Using operative outcome to assess technical skill.

BACKGROUND: This study examined whether an operative product and time to completion could serve as measures of technical skill. METHODS: Nine final-year (PGY5) and 11 penultimate-year (PGY4) general surgery residents participated in a 6-station bench model examination. Time to completion was recorded. Twelve faculty surgeons (2 per station) evaluated the quality of the final product using a 5-point scale. RESULTS: The mean interrater reliability was 0. 59 for product quality. Interstation reliability was 0.59 for analysis of the final product and 0.72 for time to completion. There was 63% and 78% agreement between attendings' ratings and product quality and time scores respectively. PGY5s' mean product quality score was 4.14 +/- 0.26, compared with 3.82 +/- 0.33 for PGY4s (P < 0.05). PGY5s' mean time was 110 +/- 19 minutes compared with PGY4s' 132 +/- 15 (P < 0.05). CONCLUSIONS: Analysis of the operative end product and time to completion offer efficient alternatives to on-line examiner scoring for bench model examinations of technical competence.

Benchmarking↗

Impact of red cell salvage on transfusion requirements during elective abdominal aortic aneurysm repair.

Elective abdominal aortic aneurysm (AAA) surgery may result in substantial blood loss. Concerns regarding the safety, availability, and acceptability of homologous blood have led to initiatives toward reducing transfusion requirements at the time of aneurysm repair. This study was designed to determine if the routine use of intraoperative red cell salvage and autotransfusion resulted in a reduction in homologous transfusion at our institution. A retrospective review of elective AAA repairs in the years 1987, 1992, and 1997 was carried out. Demographic data, operative details, blood loss, hemoglobin levels, red cell salvage and return volumes, and transfusion requirements were recorded and compared across the study years. From this study we conclude that routine use of red cell salvage and autotransfusion is an effective means for reducing transfusion requirements in elective AAA repair.

Aged↗

Comparing the psychometric properties of checklists and global rating scales for assessing performance on an OSCE-format examination.

PURPOSE: To compare the psychometric properties of checklists, global rating scales preceded by a checklist, and global rating scales alone in assessing surgery residents' performances on an OSCE-like technical skills examination. METHOD: In 1996, 53 general surgery residents with one to six years of postgraduate training participated in a performance-based examination of technical skills consisting of eight 15-minute stations (bench-model simulations of operative procedures in general surgery). Two qualified surgeons marked at each station, one using a task-specific checklist (C) and a subsequent global rating scale (Gc), the other using a global rating scale only (G). RESULTS: Interstation reliabilities measured by Cronbach's alpha were .79 for C, .89 for Gc, and .85 for G. A series of multiple regressions predicting level of training from test scores revealed an R2 of .584 for C alone, which increased to .711 when Gc was entered after (p < .001), and increased to .704 when G was entered after C (p < .001). However, R2 for Gc alone was .711, and for G alone was .704, neither of which changed when C was entered into the prediction (p > .10). The R2 for Gc and G predicting level of training (.725) was not significantly greater than that of either Gc or G alone. A very similar pattern of results was seen when C, Gc, and G were used to predict independent evaluations of the operative outcomes. CONCLUSIONS: Global rating scales scored by experts showed higher inter-station reliability, better construct validity, and better concurrent validity than did checklists. Further, the presence of the checklists did not improve the reliability or validity of the global rating scale over that of the global rating scale alone. These results suggest that global rating scales administered by experts are a more appropriate summative measure when assessing candidates on performance-based examinations.

Educational Measurement↗

UV-triggered main-component fraction collection method and its application for high-throughput chromatographic purification of combinatorial libraries.

A maximum-seeking, algorithm-driven fraction collection method was developed to support high-throughput chromatographic purification, which provides new possibilities for off-line high-performance liquid chromatography mass spectroscopy (HPLC/MS) quality control experiments. The method is based on manipulation of a six-port valve that is installed downstream from the UV detector and equipped with a fraction collector loop. The detector signal is monitored by a programmable microcontroller that controls the state of the fraction collector valve. After detecting a chromatographic peak, the appropriate fraction is stored in the collector loop. The height of the next peak is compared to the previous one (using a maximum-seeking algorithm) and, depending on the result, the collected fraction is or is not exchanged with the new one. At the end of the run, the stored UV main component is pumped into the external fraction vial. This configuration was used for chromatographic purification of large compound libraries (the results of the purification of 5324 compounds are reported here), as well as for high-throughput off-line HPLC quality control experiments, where the collected main component fractions of an analytical-scale separation were subjected to further mass spectrometric molecular weight verification.

Algorithms↗