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D T Holmes

Publications and source records attributed to D T Holmes.

3 recordsLinked to original sources

Preliminary evidence of psychological distress among reservists in the Persian Gulf War.

This study was conducted as a preliminary investigation into the presence and nature of psychological distress among military reserve personnel as a result of their participation in the Persian Gulf War. Eleven months after cessation of hostilities in the Gulf War, a self-report survey was mailed to the home of each of the 1090 members who had been assigned to the study Air National Guard unit during this period. After unit activation in December 1990, 517 of these individuals were deployed to the Persian Gulf as participants in Operation Desert Storm. The remainder of the unit participated in their military service during this period without being deployed to the Persian Gulf. The survey consisted of a demographic section, the Mississippi Scale for Combat Related Posttraumatic Stress Disorder (M-PTSD), the revised Symptom Checklist 90 (SCL-90-R), and an anecdotal response section; 46% of those surveyed responded. The major finding of the study was that 6.8% of the respondents who served in the combat theater had elevated M-PTSD scores. This was a statistically significant finding compared with the 1.7% of those surveyed who had elevated M-PTSD scores having served at home (chi2 = 6.25, df = 1, p = .01). These elevated M-PTSD scores were found despite low levels of traditional combat stressors and strong levels of perceived public support. SCL-90-R scores were also higher in deployed versus nondeployed respondents. Although the clinical presence of PTSD was not established by this study, the preliminary finding of elevated M-PTSD scores in the deployed group is suggestive of the possibility of clinical PTSD. This finding supports the need for further PTSD research among reservists who are exposed to nontraditional combat stressors. Elevated SCL-90-R scores in the deployed group also suggest that other forms of psychological distress may have developed in a significant number of combat veterans of the Persian Gulf War.

Adult↗

Cell surface oligosaccharide modulation during differentiation: III. Lectin affinity class distributions.

In previous studies we showed that the onset of the morphological phenotype of cellular senescence (IMR-90) in vitro is preceded by complex cell surface changes. Using fluorescently conjugated lectins these studies showed: (1) quantitative PDL-dependent decreases in cell surface mannosyl, galactosyl, and N-acetyl-glucosamine residues; (2) these changes were correlated with changes in ligand/lectin membrane mobility, suggesting a functional relationship for the quantitative changes. To further investigate the biological significance of these observations we have developed a synthetic ligand competition assay to analyze the lectin binding event itself. The results of these analyses show that: (1) the number of binding affinity class distributions is highly restricted; (2) the PDL-dependent mannosyl change is due to the loss of a high-affinity class distribution without significant change in the low-affinity site; and (3) PDL-dependent changes in both galactosyl and N-acetyl glucosamine binding events are the result of changes in the affinity class distributions. These results are interpreted in terms of the potential available energy to act as the basis for signal transduction at the cell surface.

Binding, Competitive↗

Cell surface oligosaccharide modulation during differentiation: IV. Normal and transformed cell growth control.

In previous studies it was shown that cell surface oligosaccharide affinity class distributions and binding capacities were down-regulated as normal cells approach senescence. Using a sensitive, amplified, lectin/specific-ligand competition analysis three other growth regulation states were compared to that of cellular senescence. Non-senescent and senescent low-density and contact-induced growth inhibition was compared with neoplastic cell growth control. Non-senescent human fetal lung fibroblasts (IMR-90) down-regulated their mannosyl and galactosyl specificities in response to both low-density and contact-induced growth inhibition. Senescent IMR-90 down-regulate their mannosyl residues in response to contact conditions while they up-regulate their galactosyl residues under the same conditions. Growth-transformed transplantable canine glioma cells did not show density-dependent regulation of their cell surface oligosaccharide structures. Modulation of the CG cells with a specific alpha-mannosidase II inhibitor, Ricinus communis a galactosyl specific lectin, and pokeweed mitogen a cellular differentiating agent resulted in an altered growth phenotype and up-regulation of the mannosyl and galactosyl surface oligosaccharides. These data indicate a controller function for the cell surface oligosaccharides and a general influence on growth control.

Alkaloids↗