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D T Rowlands

Publications and source records attributed to D T Rowlands.

At least 55 records · Page 3Linked to original sources
PubMed1975

Determinants of the hierarchy of humoral immune responsiveness during ontogeny.

A model system of ontogeny was utilized to investigate the development of humoral immunity in both AKR and BALB/c mice. Lethally irradiated adult mice were reconstituted with syngeneic fetal or neonatal liver. These mice were immunized at various times after reconstitution with a series of eight antigens: the bacteriophages F2, phiX-174, and T4; the hapten carrier complexes 2,4 dinitrophenyl-bovine serum albumin and fluorescein-bovine serum albumin; and the small proteins: hen egg lysozyme, sperm whale myoglobin, and bovine pancreatic ribonuclease. Subsequent antibody production to the antigens was assayed with either a direct or a modified bacteriophage neutralization technique. Individual mice responded to the various antigens in a sequential pattern which was basically the same for all mice within each strain. However, there was a marked difference between the two strains in the time at which they developed responsiveness to myoglobin. In order to begin to delineate the separate roles played by B and T cells in the generation of this hierarchical response pattern during ontogeny, the development of anti-DNP and anti-FTC activity was examined in carrier-primed mice. Results of this experiment indicated that functional B cell specificities for the two haptens arise at different times during ontogeny. Further studies are needed to determine whether the hierarchical pattern of immune responsiveness observed for the other antigens is a function of sequential appearance of B cell specificities, T cell specificities, or both.

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PubMed1974

Evidence of the alloimmunogenic potential of donor periodontal ligament.

The alloimmunogenic potential of each of the tooth graft components (periodontal ligament, mineralized root surface and passenger leukocytes) was evaluated. The model employed was orthotopic tooth allografts in inbred strains of Syrian hamsters. Isolated allogenic mineralized root surface is not immunogenic, as was demonstrated by the inability of tooth grafts to stimulate accelerated rejection of a subsequent skin graft if the tooth had undergone pretransplant enzymolysis of its periodontal ligament. Similarly, tooth grafts were rendered nonimmunogenic if exposed to repeated freezing and thawing. In contrast, isolated allogenic periodontal ligament on a syngeneic tooth graft was shown to evoke an accelerated rejection of skin grafts. Thus, the normal median survival time of skin (11.3 days) in the strain combination of hamsters employed was reduced to 7.4 days following exposure to an orthotopic tooth graft in which the periodontal ligament was the only obvious source of alloantigen. Prior elimination of a tooth allograft's passenger leukocytes by lethal whole body gamma-irradiation did not affect the graft's ability to sensitize the host.

Animals↗