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D TAYLOR-ROBINSON

Publications and source records attributed to D TAYLOR-ROBINSON.

At least 19 recordsLinked to original sources
PubMed1965

HEMOLYSIN OF MYCOPLASMA PNEUMONIAE.

Somerson, Norman L. (National Institute of Allergy and Infectious Diseases, Bethesda, Md.), Robert H. Purcell, David Taylor-Robinson, and Robert M. Chanock. Hemolysin of Mycoplasma pneumoniae. J. Bacteriol. 89:813-818. 1965.-Discrete colonies of Mycoplasma pneumoniae or M. laidlawii on agar produced complete (beta) lysis of guinea pig erythrocytes. Crowding of colonies on agar plates, omission of yeast extract from the medium, and incubation under reduced oxygen tension inhibited hemolysin production. Colonies which were not viable after ultraviolet radiation or heating at 56 C did not produce hemolysin. In addition, hemolysis was suppressed in old cultures in which viability was diminished, and was eliminated when colonies were removed from the agar surface by micromanipulation. The hemolysin passed through a viscose dialysis membrane. These findings suggest that the hemolysin is labile, continuously released by the colonies, of low molecular weight, and probably nonprotein in nature.

Agar↗
PubMed1963

SEROLOGICAL RELATIONSHIPS AMONG HUMAN MYCOPLASMAS AS SHOWN BY COMPLEMENT-FIXATION AND GEL DIFFUSION.

Taylor-Robinson, David (National Institutes of Health, Bethesda, Md.), Norman L. Somerson, Horace C. Turner, and Robert M. Chanock. Serological relationships among human mycoplasmas as shown by complement-fixation and gel diffusion. J. Bacteriol. 85:1261-1273. 1963.-Antigenic relationships among human mycoplasmas were studied by complement-fixation and agar gel diffusion techniques. Four recognized human species Mycoplasma hominis type 1, M. hominis type 2, M. salivarium, and M. fermentans were antigenically distinct in these tests. In addition, M. pneumoniae (Eaton agent, the etiological agent of cold agglutinin-positive atypical pneumonia) was different from these four species. Although these species were distinct, evidence of shared antigenic components was obtained in complement-fixation and agar gel diffusion tests. Since rabbits were immunized with mycoplasmas grown in rabbit muscle infusion broth supplemented with rabbit serum or, in the case of M. pneumoniae, with infected chick embryo lung suspension, the possibility that the heterologous reactions resulted from antibody to growth medium components could be excluded. Four recent mycoplasma isolates from the oropharynx were analyzed, and three were shown to be closely related to M. hominis type 1, and the fourth was closely related to M. salivarium. Although the recent isolates could not be distinguished from the related "prototype" human species by complement-fixation, differences could be detected by the agar gel diffusion technique.

Agar↗