Biomedical subjects
Publications and source records attributed to D Tavernier.
The prothombin complex is adsorbed from plasma or cryoconcentrate supernatant onto DEAE-sephadex A50. The adsorbent is washed several times with 0.21 M NaCl. The first washing proved to be a new subfraction of routine fractionation suitable as starting material for the purification of several proteins such as C1-inactivator, N-carboxypeptidase, ceruloplasmin and kallikrein.
Cholinesterase (EC 3.1.1.8) for intravenous use has been obtained from fraction IV, a by-product of ehtanol fractionation. A four-step procedure has been developed associating precipitation by Rivanol and PEG 4000 with chromatography on DEAE-cellulose. The yield corresponds to 30--35% of the activity found in fraction IV. Vials containing 5000 U of CHE stabilized by 2.5% albumin have been prepared. The pyrogen-free product is well tolerated in the human.
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