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D Timm

Publications and source records attributed to D Timm.

At least 19 recordsLinked to original sources
PubMed2005

[Analysis of bulk milk samples using polymerase chain reaction: an additional tool for bovine viral diarrhea monitoring].

Programmes for the eradication and control of infections with bovine viral diarrhea virus (BVDV) concentrate on the identification and elimination of persistently infected (PI) animals. The identification of these animals is mainly based on the detection of viral antigen using ELISA techniques. Protocols detecting viral nucleic acid using RT-PCR have been described recently. Due to high costs the German model recommends screening of animals of 9 up to 36 months of age. Screening of bulk milk samples using RT-PCR technology would allow a system independent of age. The aim of the present study was to test whether bulk milk samples (1433 including max. 50 animals each) collected in four counties of Lower Saxony are suitable for a complementary identification of PI animals via RT-PCR. Thirty-one bulk milk samples derived from 27 dairy herds were BVDV positive, corresponding to 2.3 % of the herds analysed in this study. Two samples first scored doubtful. Follow up tests revealed lactating PI animals in most cases (18). In other cases the epidemiological status of the herd, i.e. high sero-prevalence and/or presence of PI animals among non-lactating cattle, suggested a transient infection detected in the first bulk milk sample. These results demonstrate that monitoring of lactating cattle of any age using RT-PCR is a very sensitive, economically effective additional method for the identification of PI animals.

Animals↗
PubMed1999

[Primary osteosarcoma of the thyroid gland].

Primary extraosseous osteosarcoma of the thyroid gland is a very rare tumor with a poor prognosis. Only few cases have been reported in the literature. Metastases of osteosarcoma in the thyroid gland were also found but are very seldom. A primary thyroid tumor with osteosarcomatous differentiation in a 82-year-old man is presented and the histopathological classification, biological features and possible mechanisms of the occurrence of such thyroid tumors are briefly discussed.

Aged↗
PubMed1995

The crystal structure of the N-terminal SH3 domain of Grb2.

The 3-D structure of the N-terminal SH3 domain of the regulatory protein Grb2 has been determined by X-ray analysis at 2.8 A resolution and refined to a crystallographic R factor of 21.5%. The structure, which is very similar to those of other SH3 domains, consists of two orthogonal, antiparallel up-down beta-sheets, with three variable loops and a 3(10) helix. Docking of the proline-rich peptide, 3BP1 on Grb2-N SH3, shows that the polyproline type II helix can bind the SH3 domain forming conserved hydrogen bonds between the main-chain carbonyl oxygens of Met4 and Pro7 of the proline-rich peptide and the reoriented side-chains of Trp36 and Asn51, respectively, and a hydrogen bond between the main-chain carbonyl of Leu8 of the proline rich peptide with the side-chain OH of Tyr52 of the Grb2-N SH3. The peptide side-chain binding occurs on the surface of SH3 domain at three major sites involving the side-chains of the residues in the hydrophobic patch (Tyr7, Phe9, Trp36, Phe47, Pro49 and Tyr52) and the RT-Src and n-Src loops of the SH3 domain. The proline-rich peptides could bind the Grb2-N SH3 in either orientation and maintain the key hydrogen bonds because of the pseudo-symmetry of the polyproline type II helix. However, for the mSos1 peptide a salt bridge can be formed between the arginine of the proline-rich peptide and the protein at Asp15, Glu16 and Glu31 only in one direction; this orientation seems to be strongly preferred. The conservatively varied RGD sequence motif (sometimes KGE or KGD) in SH3 domains might be involved in interactions at the cell membrane.

Adaptor Proteins, Signal Transducing↗
PubMed1994

Crystal structure of the pleckstrin homology domain from dynamin.

The pleckstrin homology (PH) domain is a conserved module present in many signal transducing and cytoskeletal proteins. Here we report the 2.8 A crystal structure of the PH domain from dynamin. This domain consists of seven beta-strands forming two roughly orthogonal antiparallel beta-sheets terminating with an amphipathic alpha-helix. The structure also reveals a non-covalent dimeric association of the PH domain and a hydrophobic pocket surrounded by a charged rim. The dynamin PH domain structure is discussed in relation to its potential role in mediating interactions between proteins.

Amino Acid Sequence↗
PubMed1993

[Ectopic thyroid gland tissue in the liver].

Heterotopia of thyroid gland can be seen very seldom in humans. Most frequently ectopic thyroid tissue is found in the middle line of neck and chest. Ectopia of thyroid tissue caudal of the diaphragm is an exceptionally rare finding. An explanation results out of the embryology of the thyroid gland development. Reviewing the literature we could find only one case of accessory thyroid tissue in the region of the hepatic porta, it was found at autopsy. In the present case report we describe a 58-year-old woman who had an indistinct tumor in the hepatic porta which was successfully removed. Histologic findings was that of aberrant normal thyroid tissue.

Choristoma↗
PubMed1992

[Persistent BVD virus infections in a cattle breeding facility--a case report].

Comprehensive serological and virological monitoring for bovine viral diarrhoea (BVD) virus was applied in a dairy herd. Out of 83 calves 26 persistently infected animals were identified. Four viremic calves showed clinical signs of disease, the others displayed no symptoms. Viral isolates from persistently infected animals were homogenous with respect to their antigenicity. The results of virological and serological investigations allowed an almost complete reconstruction of events following the introduction of BVD virus into the herd. This case illustrates the potentially dangerous and damaging effects of unidentified virus carriers in cattle herds. Strategies for the identification of virus-shedding animals and the limitation of economical losses are discussed.

Animals↗
PubMed1984

[Quantitative histochemical studies of islands of Langerhans in the (Psammomys obesus) in the development of diabetes mellitus. II. Surface measurement of the islands].

Measurement of the islet-area are pointed out in 30 sand rats. The animals are divided in 3 groups: 8 sand rats are the reference group, 11 animals are the IGT-group (impaired glucose tolerance), and the diabetic group consists also of 11 sand rats. The islet-area is in the IGT-group increased in 173.2%. It is concluded that the islet-volume is also increased. The diabetic group is characterized by the maximal increase of the islet area (431.4%). Hyperplasia of Langerhans' islets is detectable histologically. The sand rat is a biological model of the human non-insulin-dependent diabetes mellitus (NIDDM).

Animals↗
PubMed1984

[Quantitative histochemical study of pancreatic islets of sand rats (Psammomys obesus) in diabetes mellitus I. Cytophotometric measurement of insulin content].

The insulin-content in islets of Langerhans on sand rats with disturbances of the carbon hydrate tolerance (reference-, impaired glucose tolerance-, and diabetic group) is measured cytophotometrically. In the IGT-group (impaired glucose tolerance) is a decrease of the insulin content in B-cells detectable. However in the diabetic group of sand rats is the insulin-content increased. This results demonstrate the stimulation of the B-cell function in the islets of Langerhans. The diabetes mellitus of sand rats is characterized by disturbed glucose tolerance hyperinsulinaemia, and elevated readiness to insulin secretion. That are characteristics of the human type-II-diabetes (insulin-independent). In conclusion, the sand rat is a biological model of the human type-II-diabetes (NIDDM).

Animals↗
PubMed1982

Body height in Turner's syndrome.

Disease-specific growth curves for Turner's syndrome were calculated by means of 2nd order homogeneous differential equations with constant coefficients, taking different cytogenetical subtypes into account. Comparison of these growth curves between X-monosomic (n = 64) and 46,XX/45,X-mixoploid (n = 20) patients reveals no differences, in contrast to the commonly accepted opinion.

Body Height↗
PubMed1979

[The application of quantitative methods in experimental morphology].

Quantitative analysis has assumed great importance for describing and appraising evolutionary and involutionary processes. Measurements alone do not permit conclusions regarding the laws governing natural development. The elaboration of biologically meaningful mathematical models is necessary for such purposes. This paper shows how functions with a terms can be used to describe skull and body growth under normal and experimental conditions (frontal biped model in Rattus norvegicus Berkenhout as a biological model). A model consisting of a 2nd order homogenous linear differential equation with constant coefficients has proved to be biologically meaningful for describing skull length and breadth, and for the gross body weight. In order to study different growth rates in control and experimental animals we have calculated "confidence intervals" for the graphs of the special solutions of the general integral. This permits optimum validity although no mathematically precise interpretation can be given.

Animals↗
PubMed1975

[Infected groin following vascular surgery].

From August 1972 to July 1973 174 vascular reconstructions in the iliac and femoro-popliteal region were performed. All infections of the groin incision were classified according to Szilagyi. 22% class I and II infections, 2% class III infections were found (3 patients). In two of these patients the bleeding was managed by the following method: The infected piece of the artery was resected. The vascular continuity was reconstructed by autologous vein. The anastomoses were burried in uninfected surrounding muscles. Both patients left the clinic with open leg arteries. Unfortunately we lost the third patient doing an extraanatomical bypass. The reasons for the high incidence of groin infections are discussed.

Arterial Occlusive Diseases↗