PubMed Health⌕ Search

Biomedical subjects

D Todorov

Publications and source records attributed to D Todorov.

8 recordsLinked to original sources
PubMed1979

[Change in ion transport across the epithelium of the frog skin isolated from the body].

The authors carried out measurements of ionic transport through the epithelium on preparations of isolated and nonisolated skin by the method of Ussing and Zerahn (short-circuiting technique). The blood and nervous connection with the body was preserved in some of the skin preparations (nonisolated) during the experiment. For the purpose the two chambers of Ussing were modified in such a way that this connection could not be interrepted becuase of compression of blood vessels and nerves. Comparative measurements showed that isolation of the skin from the body of a frog caused significant reduction of active transport of ions through the skin epithelium. After addition of acethylcholine, eserine or dipterex to the solution of the internal or external surface of the preparation the nonisolated skins revealed statisticaly not proven tendency to greater reactivity.

Acetylcholine↗
PubMed1978

[Experiment to potentiate the effect of cyclophosphamide combined with tempidon and caffeine in leukemia L1210 and Lewis hepatic carcinoma].

The authors carried out experiments on mice DBA/2 and BDF1 with leucosis L1210 and lung carcinoma of Lewis and found that psycotropic preparations tempidone and caffeine did not manifest antitumorous effect and toxicity after the used doses and scheme of treatment. Tempidone enhanced antitumorous action of small and mean doses of cyclophosphamide in mice with leucosis L1210. In the triple combination (tempidone, caffeine and cycl phosamide) the caffeine enhanced this action of tempidone without raising toxicity in mice with leucosis L1210, but not in mice with lung carcinoma of Lewis.

Animals↗
PubMed1977

[Comparative studies of methods for the bacteriological diagnosis of mastitis in cows].

Comparative studies were carried out on some methods and nutrient media used for the isolation and identification of mastitis causative agents. Milk from normal andmastitis-affected udder quarters was aseptically sampled and treated under various conditions. Individual samples were simultaneously seeded in different media. The following conclusions were drawn: 1. Milk samples for the diagnosis of mastitis of a subclinical character can be taken at the beginning and at the end of milking. 2. Direct seedings of milk have equal diagnostic value as those of the sediment. With the former the process of bacteriologic treatment of the samples is shortened in time, and the isolation and differentiation of the mastitis agents is rendered feasible. 3. At the present etiologic structure of the subclinical types of mastitis in this country most suitable has proved the blood agar medium in which almost all mastitis causative agents can be demonstrated. 4. Kartashova's medium and the TKT-medium possess a pronounced selectivity with special reference to streptococci and have higher diagnostic value for farms where streptococcus mastitis is prevailing.

Animals↗
PubMed1977

[Sensitization to mites in house dust among children suffering bronchial asthma].

The results of studying allergy to domestic dust in 168 patients suffering from bronchial asthma are presented. The diagnosis was made by means of the domestic dust allergen (10000 PNU), fraction E extracted from the whole allergen by Berrens and Young's method, and with lyophilized extract from D. pteronussinus, farinae and Glycyphagus ticks isolated from domestic dust by Voohorst and Spieksma's method. In parallel, IgE was determined in the blood serum of the patients under study by the radio-allergo-sorbent test; IgG was also determined. Microticks proved to be not the only factor determining the activity of domestic dust. Results of diagnosis in vivo and in vitro were found to correlate. The authors believe that the activity of domestic dust allergen formed on account of the reactions of the Maillard's type, in which a significant role was played by the "lysine-sugar) compounds.

Adolescent↗
PubMed1976

[Coliform microflora of commercial Caucasian cheese].

Studied was in the course of several years the extent of coliform bacterial contamination and the peculiarities the bacteria present in ripened cow's and ewe's white brine cheese offered for consumption. It was demonstrated that these kinds of cheese released in the commercial system contain up to 3.1 per cent coliform bacteria. Certain correlation was established between the coliform count in the cheese and the degree of ripening, the consistence, and the aspect of the cross section surface. If hygiene measures were not observed cheese could be contaminated additionally with coliforms during handling in the shops. Coliform microflora was presented mainly by strains characteristic of fresh fecal contamination (E. coli) and urease-positive organisms of the Citrobacter and Enterobacter genera. To guarantee the consumption of qualitative (in terms of hygiene) white brine cheese it is necessary to include into the respective standards the index "coli titer" of a unit value.

Animals↗
PubMed1976

[Effect of acetylcholine on active ion transport across the epithelium of frog skin].

The effect of acetylcholine on isolated skin of Rana ridibunda is studied. The application of acetylcholine (1 mM) in Ringer's solution, washing the outer aspect of the skin, causes slow and smooth increase in the preparation's current and potential. Upon acetylcholine addition to the solution on the inner aspect, the increase of the above parameters runs a course along a two-peaked curve. It is presumed that in the latter case acetylcholine, prior to exert a direct effect on the epithelium, influences some of the nerve or glandular formations in the skin corium.

Acetylcholine↗