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D Toth

Publications and source records attributed to D Toth.

10 recordsLinked to original sources
PubMed2003

[Long-term follow-up after complete treatment of peritoneal endometriosis with the CO2 laser].

OBJECTIVE: To evaluate the effect of laparoscopic CO2 laser ablation of peritoneal endometriosis in the treatment of pelvic pain with a long-term follow-up. To differentiate the effect of surgery on different types of pelvic pain. DESIGN: Prospective observational study. SETTING: Department of Obstetrics and Gynecology, 1st Medical Faculty, Charles University and General Faculty Hospital, Prague. METHODS: Patients with 1st to 3rd stage endometriosis, with manifestation of pelvic pain, and with complete excision of peritoneal endometriosis lesions, were included in the study. All visible lesions were vaporized by CO2 laser following adhesiolysis and complete visualization of the pelvis. After the procedure, patients were followed up at 6-month intervals. The severity of pelipathia, dyspareunia, dysmenorrhea, pain during micturition, and pain during defecation were monitored using a visual analog score of 10 points. RESULTS: A total of 31 patients were included in the study. After 6, 12, and 18 months after surgery, the recurrence of pelvic pain was found in 12 (39%), 15 (48%), and 19 (61%) patients, respectively. Improvement or disappearance of complaints was documented 18 months after the surgery in 11 cases of dysmenorrhea (50%), 9 cases of dyspareunia (50%), 14 cases of pelipathia (58%), 12 cases of pain during micturition (71%), and in 14 cases of pain during defecation (87.5%). The proportion of recurrences increases with the length of the interval after the procedure, mainly in dysmenorrhea and dyspareunia. CONCLUSIONS: The effect (improvement or disappearance of pelvic pain) of a complete CO2 laser ablation of peritoneal endometriosis continues 18 months after the surgery in about 40% of patients. A graduated increase in the number of recurrences is apparent during follow-up, most significantly in dysmenorrhea and dyspareunia. The effect of surgery on different types of pelvic pain varies. A small number of recurrences was found in pain during micturition and pain during defecation, on the other hand, less success was apparent in the treatment of dysmenorrhea.

Adult↗
PubMed2002

Human cerebral perfusion analysis with ultrasound contrast agent constant infusion: a pilot study on healthy volunteers.

With ultrasound (US) contrast agent (UCA) continuous infusion providing a steady state, mean tissue microbubble velocity can be assessed by analyzing the reappearance rate after microbubble destruction with US energy (refill kinetics). In this study, we investigated this new approach for the assessment of human cerebral perfusion. A total of 12 healthy volunteers were investigated transtemporally with increasing pulsing intervals (250, 500, 750, 1000, 1250, 1500, 2000, 3000 and 4000 ms) and two UCA infusion rates (0.5 and 1.0 mL/min of Optison). Intensity vs. pulsing interval curves were analyzed using an exponential curve fit and parameters of the curve (plateau echo enhancement, A, representing the microbubble concentration within the interrogated tissue; rate constant, beta, which is related to blood flow and their product, F = Abeta) were compared. For 20/20 investigations being available for further analysis, it was possible to generate a typical exponential intensity vs. pulsing interval curve from the ipsilateral thalamus. The plateau echo enhancement A showed a significant (p = 0.02), and the beta as well as the F values displayed a nonsignificant (p = 0.06, both), increase with infusion rate. The qualitative analysis of beta and F parameter images displayed the most homogeneous visualisation of perfusion in the ipsilateral thalamus and main territory of the middle cerebral artery. In conclusion, it is possible to display the UCA refill kinetics in human cerebral microcirculation after microbubble destruction by transcranial US. Grey-scale harmonic imaging allows a quantitative approach to cerebral perfusion with a large interindividual variation of the parameters.

Adult↗
PubMed1998

Young child injury analysis by the classification entropy method.

The project 'The Register and Preventive Programs for Accidents and Injuries' enabled data collection on all the injured who sought medical aid in Koprivnica County (population 61,052), Croatia, since 1992. Children aged 1-4 years are 5.03% of the whole population of the district. Complex injury attributes were analysed. Binary attributes were classified as input: age, gender, place of injury; and output: severity of injury. A new application of information entropy was introduced and applied to the classification of injury-causes attributes. The information entropy was calculated for the classification of input attributes according to the minimum information content. The decision procedure is given as a sequential procedure separating important from unimportant causes of injury at each decision level. Thus a decision tree with increasing entropy, i.e. decreasing determinism, was obtained showing that age (0.5347 N), place (0.6062 N) and gender (0.6105 N) are measurable attributes in child injury ascertainment in a descending pattern. It was shown that this method is, at the same time, an optimal way of using an attribute decision process of injury causes classification.

Accidents↗
PubMed1996

Skin and diabetes.

Skin manifestations associated with diabetes mellitus are common and, with close scrutiny, can occur in most, if not all, patients. Little information is available on what common pathophysiologic thread is responsible for the skin manifestation and diabetes. Although controlled studies are lacking, a recognition of the skin manifestations, treatment, and prognosis will help patients and their caregivers to understand treatment alternatives more clearly.

Adult↗
PubMed1994

Tcp20, a subunit of the eukaryotic TRiC chaperonin from humans and yeast.

Members of the Hsp60 chaperonin family, such as Escherichia coli GroEL/S and the eukaryotic cytosolic chaperonin complex, TRiC (TCP ring complex), are double toroid complexes capable of assisting the folding of proteins in vitro in an ATP-dependent fashion. TRiC differs from the GroEL chaperonin in that it has a hetero rather than homo-oligomeric subunit composition and lacks a GroES-like regulatory cofactor. We have established greater than 57% identity between a protein encoded by the TCP20 gene from a human cDNA library and the newly identified protein encoded by the TCP20 gene located on the right arm of chromosome IV of the yeast Saccharomyces cerevisiae. These Tcp20 proteins showed approximately 30% identity to Tcp1, a known subunit of TRiC. Gel filtration, followed by Western analysis of purified bovine testis TRiC with a Tcp20-specific antibody, indicated that Tcp20 is a subunit of the hetero-oligomeric TRiC. Gene disruption experiments showed that TCP20, like TCP1, is an essential gene in yeast, consistent with the view that TRiC is required for folding of key proteins. The amino acid sequence similarities and the derived evolutionary relationships established that the human and yeast Tcp20 proteins represent members of a new family of subunits of TRiC chaperonins.

Amino Acid Sequence↗
PubMed1983

Classroom teachers' attitudes toward children with articulatory disorders.

A group of 116 elementary and junior high school educators were asked to list terms descriptive of children with articulatory disorders. A list of 20 terms was established and then rated for desirability-undesirability by 100 college students. A majority of the terms were negative in nature and were given undesirable ratings. The results suggest that many of the educators in the sample possess a number of negative attitudes toward children with articulatory disorders.

Adult↗
PubMed1976

Fermentation of purines and their effect on the adenylate energy charge and viability of starved Peptococcus prévotii.

The principal products of endogenous metabolism of the obligate anaerobe Peptococcus prévotii were carbon dioxide, ammonia, acetate and butyrate, which are also produced by fermentation of nucleosides, purines and ribose, thus supporting our previous finding that RNA is the only cellular component to undergo substantial degradation under starvation conditions. Minor products were hydrogen, formate and propionate. The stoicheiometries of fermentation of xanthine, adenine, adenosine and ribose were determined. The ability to ferment exogenous nucleosides, purines and ribose declined rapidly on starvation, as did the ability to generate ATP from xanthine, and was paralleled by the loss of viability. However, addition of pulses of xanthine or adenine to organisms in the early stages of starvation sustained their adenylate energy charge and prolonged their viability. Evidence suggests that P. prévotii dies when its principal energy source (RNA) is depleted, and a possible explanation is that the transport function of the cytoplasmic membrane decays rapidly under these conditions so that, on transfer to a recovery medium, uptake of nutrients is inadequate to support growth.

Acetates↗
PubMedDate not supplied

Effects of ammonium and lactate on growth and metabolism of a recombinant Chinese hamster ovary cell culture.

A Chinese hamster ovary (CHO) cell line producing a recombinant glycoprotein was cultured in batch mode with different initial concentrations of ammonium chloride (0-10 mM), sodium lactate (0-60 mM), or sodium chloride (0-60 mM). High ammonium concentrations did not inhibit cell growth and productivity or glucose and glutamine consumption. In contrast, specific ammonia and alanine production decreased by 55% and 40%, respectively. There were also significant increases in specific aspartate and glutamate consumption in high ammonium concentrations. These observations indicated a shift in glutamine catabolic pathways in response to the effects of ammonium. The influence of lactate on growth and metabolism were the combined effects of lactate concentration and osmolarity. After "correcting" for osmolarity effects, lactate was found to inhibit growth by 25% but to increase specific productivity slightly (10%). Lactate had profound effects not only on glycolysis but also on glutaminolysis. While specific glucose and glutamine consumptions decreased by 15-20%, the effects of lactate on their metabolic products were far more significant. Lactate production was halted, and specific ammonia and alanine productions decreased by 64% and 70% at high lactate concentration. Theories on how ammonium and lactate affected the metabolic pathways of glucose and glutamine are presented.

Ammonium Chloride↗