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Biomedical subjects

D V Hanselka

Publications and source records attributed to D V Hanselka.

18 recordsLinked to original sources

Technique for long-term ileal cannulation in ponies.

A T-cannula was fitted permanently into the ileum of ten mature ponies to quantify the role of the prececal and postileal segments of the digestive tract in equine nutrition studies. The ponies were anesthetized, positioned in left lateral recumbency, and the distal small intestine was exteriorized through a right paralumbar incision. A silastic T-cannula was inserted through a 2-cm longitudinal incision into the lumen of the ileum, on the antimesenteric surface, and sutured to the ileum. The stem of the cannula was exteriorized through a 2-cm circular skin incision, 6 cm caudal to the 16th rib. Nine ponies were alive with functional cannulas after six months. Primary complications associated with cannulation of the ileum were clinical signs of depression and dehydration produced by leakage of intestinal contents around the stem of the cannula. These complications were resolved with fluid and electrolyte therapy.

Animals↗

Skin grafting of the horse.

Free autogenous skin grafting of the horse is indicated for wounds too large to heal by contraction and epithelization. Techniques of pinch, punch, tunnel, and sheet grafting are described. Allografting and storage of skin for delayed grafting are discussed.

Animals↗

Nasopharyngeal cicatrices in horses: 47 cases (1972-1985).

Nasopharyngeal cicatrices were observed endoscopically in 47 horses examined because of abnormal respiratory noises and/or exercise intolerance. A review of these cases revealed a correlation between cicatrization and age, sex, and the presence of other upper airway abnormalities. The age of affected horses ranged from 6 to 21 years, with a mean age of 12.7 +/- 7.8 (+/- 2 SD) years. Females were affected 2.7 times more frequently than males. Abnormalities commonly observed with a nasopharyngeal cicatrix included chondritis of the arytenoid cartilage, epiglottic deformity, and deformity of the guttural pouch openings. The nasopharyngeal cicatrix was responsible for respiratory obstruction in only 3 horses. Treatment for the cicatrix was not performed on any horse in this series. Generalized nasopharyngeal and laryngeal inflammation were postulated as causes of the cicatrices and associated abnormalities.

Age Factors↗

Repair of digital flexor tendon lacerations in the horse, using carbon fiber implants.

Flexible medical grade carbon fiber was surgically implanted in tenectomized or lacerated superficial and deep digital flexor tendons of 13 horses (7 clinical cases and 6 experimental), ranging in age from 15 months to 10 years and weighing 300 to 500 kg. The 6 experimental horses were euthanatized at 30-, 45-, 60-, and 90-day intervals for gross and histologic evaluation of the results. Three of the experimental horses served as their own controls. Of the 7 clinically affected horses, 3 were euthanatized because they developed laminitis or the wound failed to heal. The remaining 4 horses in that group were discharged; 3 returned to their previous activities, and 1 was unavailable for follow-up. Carbon fiber produced a structure of approximately the same size and function as a normal tendon. Histologically, the fibroblasts and collagen fibers organized and aligned themselves in the early stages of healing. The implants appeared grossly and histologically to produce a cosmetically acceptable, strong, and functional tendon.

Animals↗

Surgical correction of deviated nasal septum and premaxilla in a colt.

A 6-month-old Appaloosa colt had a deviation of the premaxilla and nasal septum as well as a dorsal hump of the nasal bone and maxillomandibular malocclusion. Two surgical procedures were performed 12 weeks apart to correct these anomalies. An intraoral approach and autogenous rib graft were used to correct the malocclusion and deviation of the premaxilla. Osteotomy of the nasal bone and removal of the nasal septum were performed to help correct the deviation and to facilitate free air passage through the nares.

Animals↗

Amphotericin B for the treatment of localized subcutaneous phycomycosis in the horse.

Amphotericin B was used systemically or locally, or both, in the treatment of localized subcutaneous phycomycosis in horses. In 8 of 10 cases, the results were satisfactory. Intravenous treatment was well tolerated at an average starting daily dosage of 0.38 mg/kg, increasing up to as high as 1.47 mg/kg. It was concluded that the ideal treatment regimen would include early surgical removal of the lesion followed by daily intravenous and topical administration of amphotericin B, with periodic extirpation of small necrotic tracts as necessary.

Amphotericin B↗

Preservation of skin by refrigeration for autogenous grafting in the horse.

Eighteen stored split thickness meshed skin grafts were applied to surgically created lesions on the metacarpal and metatarsal regions of six horses. Donor skin was harvested from the sternal region, meshed and stored at 4 degrees C in a cell culture medium containing 10% serum. Stored grafts were applied to the wounds at 1, 2, and 3 week intervals. Acceptance of the grafts stored for 1 week was generally poor (1 of 6 grafts), whereas that of the 2 and 3 week old grafts was generally excellent (10 of 12 grafts). Poor acceptance of the 1 week old grafts was attributed to streptococcal infection of the recipient wounds. Using the storage medium and grafting technique described, excellent acceptance can be expected after graft storage of up to 3 weeks.

Animals↗