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Biomedical subjects

D V Kulaev

Publications and source records attributed to D V Kulaev.

At least 19 recordsLinked to original sources
PubMed1995

[Specific hemosorption, kinetics and physiological effects].

The paper covers the physicochemical and clinicophysiological aspects of hemosorption. It proposes mathematical models of blood elimination kinetics of substances which have different affinities for plasma proteins and cellular receptors. It also describes the processes occurring onto the surface of hemosorbents on their contact with blood. It is attempted to associate these processes with some immunomodulating effects of hemoperfusion.

Blood Cells↗
PubMed1992

[The affinity lavage of the abdominal cavity in diffuse peritonitis with liquid sorbents based on cross-linked dextrans].

Experiments were conducted on 35 Wistar rats with generalized purulent peritonitis. Twenty-four hours after infection the authors introduced into the animals' abdominal cavity gels of cross-linked dextrans with immobilized polymixin (50 mg/g dry weight) and terrilithin (100 PE/g dry weight), as well as a combination of these sorbents with 24-hour exposure in a 5 ml volume. The object of the study was the detoxification effect. The authors revealed a phenomenon of agglutination of red cells of an ox or rat sensitized by plasma of sick animals in mixing with sephadex G-200 granules with polymixin W immobilized on them. The agglutination titers determined the activity of the sorbent taking part in lavage of the abdominal cavity. The study showed that cross-linked dextrans with immobilized polymixin B and terrilithin introduced into the abdominal cavity in peritonitis are capable of extracting substrates possessing affinity to them, the polymixin sorbent raises the quality of careful mechanical lavage. Combination of gels based on cross-linked dextrans with enzymatic and affinity functions in relation to toxins leads to detoxification and restoration of the disturbed biochemical values by 87%.

Abdomen↗
PubMed1992

[Possibility of selective binding of HIV-1 infected cells by immunosorbents].

A study was made of a possibility of specific sorption of HIV-1 infected cells with gamma-fraction of AIDS patients' serum containing high titer-specific antibodies, immobilized on the silica matrix (C-3). The laboratory tests were made with the use of the monocyte culture [symbol: see text] chronically infected with HIV-1. In addition to a decrease of the cell count after sorption, there was a decline of the activity of antigen material in the samples (to 43% of the initial). The selectivity rates were 0.92 for immunosorbents and 0.29 for sorbents of the control group, that confirms and increase of the delay of the cells expressing HIV-1 proteins on a column with the immobilized gamma-fraction of AIDS patients' serum. The use of the synthesized immunosorbents in vivo may appear debatable. However, this method can be employed for preparative purposes.

Acquired Immunodeficiency Syndrome↗
PubMed1991

[Immunosorption of individual HIV proteins and virions].

Immunosorbents specifically binding native (gp160, gp120, gp41) and recombinant env proteins and HIV-I virions were synthesized on the basis of Sepharose 4B and Silica with immobilized ligands such as gamma-fraction of rabbit antiserum to HIV-I proteins and purified antibodies to env proteins of HIV-I. The possibility was shown of selective extraction of HIV-I virions and individual HIV proteins both in vitro and in vivo. The titer of virus antigens (in ELISA) after perfusion via an immunosorbent of patterns with a high content of virions and HIV-I proteins was 8 times as low as the starting titer (after perfusion via the control sorbent it was 2-fold decreased). Extracorporeal immunosorption in animals after intravenous injection of recombinant env protein permitted the latter's titer to be 5 times lower. After perfusion via the control sorbent the titer dropped by at least 20% as compared with the starting titer. The possibility of using immunosorption in multimodality therapy of AIDS is under discussion.

Acquired Immunodeficiency Syndrome↗
PubMed1991

[Protective effect of anti-staphylococcal immunosorbent on the phenotype of lymphocyte membrane in diffuse Staphylococcus-Pseudomonas peritonitis].

An antistaphylococcal and an antitetanic immunosorbents on a silica carrier were tested in diffuse staphylococcal pyocyanic peritonitis to protect receptor expression on the general T and B lymphocyte populations and of Fc-receptors to IgM, IgG and IgA. Plasma of animals with peritonitis was shown to exert a powerful suppressive effect on lymphocyte receptors. Perfusion of toxic plasma over the antistaphylococcal immunosorbents resulted in specific adsorption of microbial toxins and completely prevented Fc gamma-, Fc mu- and Fc alpha-receptor expression on cells. Plasma treated with the antitetanic immunosorbent retained suppressive effects. The low protection of receptors may be explained by nonspecific adsorption of texins.

Animals↗
PubMed1991

[The immunomodulating properties of staphylotoxin].

The action of native staphylotoxin has been tested on T lymphocytes obtained from 21 healthy human donors. As revealed in this investigation, toxic action affects Fc-receptors to IgG, IgM and IgA. The dose-dependent effect of the preparation suggests its direct contacts with Fc-receptors of lymphocytic membranes. High doses of the toxin suppress the expression of Fc-receptors 6- to 12-fold, low doses of the toxin are capable of stimulating the expression of Fc gamma-receptors.

Adjuvants, Immunologic↗
PubMed1986

[Affinity hemosorbent for the binding of atherogenic lipoproteins].

An affine hemosorbent, a product of covalent heparin immobilization on a solid matrix, has been proposed for the extraction of atherogenic lipoproteins. The sorbent has high capacity with respect to atherogenic lipoproteins and is selective with other blood constituents. Experimental hemosorption in animals demonstrated rapid sorbent regeneration and satisfactory hemo- and biocompatibility, therefore the new sorbent can be recommended for clinical application.

Animals↗
PubMedDate not supplied

[The use of siliceous immunosorbents in hemosorption].

Experiments were conducted on rabbits and monkeys for approbation of immunosorbents by covalent immobilization of human serum albumin or antibodies to human apoprotein B on the surface of silo-chromium and subsequent incubation with 1% albumin solution. Extracorporeal immunosorption in immunized rabbits led to reduction of the level of specific antibodies by 60% and the number of blood platelets by 10-30%. The initial antibody level was restored in 5-10 days. Study of immunosorbents on monkeys showed a satisfactory compatibility of the sorbent with blood, its ability for regeneration, and the possibility of its repeated use. The specific capacity was 66% of the capacity according to human apoprotein B.

Animals↗