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Biomedical subjects

D V Stefani

Publications and source records attributed to D V Stefani.

At least 19 recordsLinked to original sources
PubMed1994

[Types of immunocompetent cell reactions in in vitro loading tests in healthy children].

Lymphocyte reactivity was assessed with various surface antigens detectable by Russian monoclonal antisera by indirect immunofluorescence in peripheral blood samples from 40 healthy children in vitro in loading tests. Values of a coefficient reflecting individual reactivity of immunocompetent cells with surface antigens CD5, CD4, and CD8 corresponding to type II reaction were found characteristic of children whatever their age or sex. The results may be used as reference values in examinations of children with various diseases.

Child↗
PubMed1991

[Nature of the reaction of the immunocompetent cells in children with nephrotic syndrome and in their parents with different HLA phenotypes].

The purpose of the study was to analyze the character of the expression of the blood lymphocyte epitopes SD4, SD8 (EBLE SD4, SD8) in a series of the loading in-vitro tests in children suffering from the nephrotic syndrome, with different HLA haplotypes. Nine children with the hormone-sensitive nephrotic syndrome (HSNS) and hormone-resistant nephrotic syndrome (HRNS) and 11 parents were examined. Before and after the in-vitro loading with medicamentous agents EBLE SD4, SD8 were determined by flow cytofluorometry, while HLA antigens were tested by the standard micro-lymphocytotoxic method. The studies allowed revealing differences in the responses of EBLE SD8 to the in-vitro loading in children with the HRNS and HSNS. The character of EBLE SD4, SD8 in a child with the NS and its parents may attest to the involvement of those antigens in the pathogenetic component of the given disease.

Adolescent↗
PubMed1989

Suppression of anaphylactic shock in sensitized guinea pigs by cytochalasin B.

Twenty-three guinea pigs, presensitized with horse serum, were injected intracardially with 1 ml of 1.5 X 10(-4) M cytochalasin B in a 2.5% dimethyl sulfoxide solution, 1.5 h before being challenged with the serum. Eighteen of the guinea pigs (80%) survived. A single intracardiac injection of cytochalasin B to the control group of guinea pigs at 1.5 h before administration of the allergen did not prevent their sensitization and death following challenge.

Anaphylaxis↗
PubMed1989

[Dynamics of inherent interactions between the classical and alternative pathways of complement activation in premature infants with suppurative-inflammatory diseases].

Altogether 57 premature infants were examined. Of these, 15 presented with neonatal sepsis, 36 with local purulent infection (LPI) of different etiology, and 6 children were conventionally normal serving as control. Early activation of the alternative pathway of the complement system was shown by the patients as compared with control. The development of neonatal sepsis was attended by the increased role played by the classical pathway of complement activation. At the same time the course of LPI was characterized by differences in the degree of participation of the classical and alternative pathways in the antiinfectious defence of the neonates. It has been established that high activation of the alternative pathway seen in the premature children may be considered as a prognostic sign enabling one to classify them with the risk group in terms of the possibility of the development in them of an infectious process.

Acute Disease↗
PubMed1987

[Hemosorption in status epilepticus and serial epileptic seizures].

Hemosorption was performed in 8 patients with a resistant status epilepticus and serial epileptic attacks; arrest of the attacks correlated with significantly faster and stabler normalization of the circulating levels of immune complexes and of the phagocytic activity of neutrophils as compared to the same parameters in patients receiving a course of conventional anticonvulsive and dehydration therapy without hemosorption. The data obtained point to the advisability of the inclusion of hemosorption into a multiple modality treatment of patients with a persistent grand mal status and serial epileptic attacks. High circulating levels of immune complexes in the blood serum may be considered as indication for hemosorption.

Adult↗
PubMed1985

[Concentration of circulating immune complexes in the blood in clinical and experimental epileptic seizures].

The levels of circulating immune complexes (CIC) in the peripheral blood were studied in 31 epileptic patients and 30 rabbits which served as an experimental model of the development of the epileptic process. CIC levels in the blood serum were measured by means of polyethylene glycol precipitation followed by measurement of optic density by a spectrophotometer. Elevated concentrations of CIC in the patients' blood were observed in the period of the epileptic status and during a series of epileptic attacks, as well as in experimental animal during epileptic attacks and in the period preceding their development. Blood analysis made at different periods after controlling the attacks showed gradual normalization of the levels of CIC. The role of CIC in the development and maintenance of epileptic seizures is discussed.

Adolescent↗