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Biomedical subjects

D V Stefani

Publications and source records attributed to D V Stefani.

90 records · Page 5Linked to original sources
PubMedDate not supplied

Suppression of anaphylactic shock in sensitized guinea pigs by cytochalasin D.

A new highly effective pathogenetic therapy for anaphylactic reaction in vivo has been suggested. Forty-three guinea pigs, presensitized with horse serum and divided into two groups, were injected intracardially with 1 ml of 1.6 x 10(-4) M cytochalasin D and 1 ml of 1.6 x 10(-5) M cytochalasin D in DMSO solution at various times before being challenged with the serum. Experimental data not only showed that anaphylactic reaction can be suppressed in vivo by blocking cytoskeletal activity but also demonstrated different pharmacodynamic characteristics of cytochalasin D on clinical course, gravity and outcome of anaphylactic shock in sensitized guinea pigs, depending on the interval between administration of the drug and allergen.

Anaphylaxis↗
PubMedDate not supplied

[The characteristics of the development of immunity with a primary immunization complex using adsorbed DTP vaccine].

The intensity of immunity, depending on immune response variants characterized by the definite composition of the T and B lymphocyte subpopulation in peripheral blood, was studied in 70 practically healthy young children after the primary complex of immunization with adsorbed DPT vaccine. The most stable immune reaction was shown to appear in children with the hyperergic and normoergic variants of immune response to diphtheria and tetanus toxoids, while the reaction to pertussis antigen was essentially weaker both after the second vaccination and after the first revaccination. In children with the hypoergic variant of immune response to all components of adsorbed DPT vaccine the resulting immune reaction was 2.5-3 times weaker than in children of other groups.

Adsorption↗
PubMedDate not supplied

[Characteristics of immune response to diphtheria anatoxin in children with different immune status during the second age-scheduled revaccination].

The dynamics of the intensity of specific antidiphtheria immunity after the second age-scheduled revaccination was studied in 129 practically healthy children. The study revealed that the formation of immunity depended on the initial functional state of the immune system before the injection of diphtheria toxoid. Three variants of immune response were determined and the immune status corresponding to each of these variants was characterized. As shown in this study, children with the hyperergic character of immune response were characterized by relatively high initial titers of antitoxin, and the injection of an additional dose of the antigen led to the prolonged state of hyperimmunization with the subsequent decrease of the intensity of immunity by half, registered in the catamnestic observation for 4 years. Children with the hypo- and normoergic variants of immune response were characterized by the most stable immune response to diphtheria toxoid, and during the catamnestic observation they formed the levels of antibody titers 2.5- to 3-fold higher than before immunization. But the protection characteristics in children with the third variant were the lowest among the children under study.

Antibody Formation↗
PubMedDate not supplied

Levels of IgD in patients with rheumatoid arthritis.

It has been proposed that IgD may have a pathophysiological role in certain autoimmune diseases, since antinuclear IgD antibodies were revealed in patients with systemic lupus erythematosus, rheumatoid arthritis and systemic sclerodermia. The studies on the problem of evaluation of serum IgD levels in patients with rheumatoid arthritis (RA) are scanty and their results are controversial. We studied IgD levels in patients with RA in order to demonstrate possible correlations with selected immunologic parameters, and to determine the relevance of IgD quantitation in the diagnosis of RA. We determined IgD levels in 30 patients with classic or proven RA, and in 30 age and sex-matched volunteers. Immunoglobulins of A, M, G classes were also determined. E-rosettes were assayed by the method of Jondal et al. in a modification. The distribution of IgD concentrations in donors revealed two modes, and also showed bimodal distributions in the patients. IgG and IgA levels were practically equal in the IgD-positive patients, but IgM was significantly lower than in the IgD-negative subjects. E-rosette values were also different in these subgroups. IgD-positive patients had significantly higher numbers of E-rosettes (1254 +/- 215), compared to IgD-negative individuals (497 +/- 146). IgD was found more often in the seropositive patients to rheumatoid factor (60%) than in seronegative ones (33%) but the difference was not statistically significant.

Adult↗
PubMedDate not supplied

[Possibilities of the use of parameters of membrane assembly of immunoglobulin receptors of B-lymphocytes in the differential diagnosis of intestinal diseases in children].

Children with different forms of enteric malabsorption were examined using an original method for assessing the parameters of the process of membrane assembling for immunoglobulin receptors of B-lymphocytes, which helps single out several additional criteria for the differential diagnosis of variants of the malabsorption syndrome. The authors consider the phase coefficients characterizing each stage and the general coefficient reflecting the regularities of the kinetics of membrane assembly of receptors in general to be the most essential. Use of this method in pediatrics offers new potentialities in the differential diagnosis, prediction of disease course, and monitoring of the treatment efficacy.

Adolescent↗