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D Villalon

Publications and source records attributed to D Villalon.

3 recordsLinked to original sources
PubMed1994

Virus-associated down-regulation of the gene encoding cryparin, an abundant cell-surface protein from the chestnut blight fungus, Cryphonectria parasitica.

We report here the cloning and analysis of the gene (Crp) encoding cryparin (CRP) and the suppression of the accumulation of its mRNA by a double-stranded RNA virus. CRP belongs to a group of fungal hydrophobic cell surface proteins and has sequence similarity to cerato-ulmin, a phytotoxin produced by Ophiostoma ulmi, the Dutch elm disease pathogen. Transcripts of the Crp gene represent almost 25% of the mRNA of the fungal cell during the peak of CRP production.

Amino Acid Sequence↗
PubMed1988

Expression of human adenosine deaminase in murine hematopoietic cells.

Multiple replication-defective retrovirus vectors were tested for their ability to transfer and express human adenosine deaminase in vitro and in vivo in a mouse bone marrow transplantation model. High-titer virus production was obtained from vectors by using both a retrovirus long terminal repeat promoter and internal transcriptional units with human c-fos and herpes virus thymidine kinase promoters. After infection of primary murine bone marrow with one of these vectors, human adenosine deaminase was detected in 60 to 85% of spleen colony-forming units and in the blood of 14 of 14 syngeneic marrow transplant recipients. This system offers the opportunity to assess methods for increasing efficiency of gene transfer, for regulation of expression of foreign genes in hematopoietic progenitors, and for long-term measurement of the stability of expression in these cells.

Adenosine Deaminase↗