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D W Choi

Publications and source records attributed to D W Choi.

228 records · Page 13Linked to original sources

Brief exposure to zinc is toxic to cortical neurons.

Recent evidence suggests that large amounts of releasable zinc are present in central synaptic vesicles, and that substantial (several hundred microM) transient elevations in extracellular zinc may accompany intense neuronal excitation. Brief pulse exposure of cortical cell cultures to similar concentrations of zinc resulted in widespread dose-dependent neuronal injury; exposure to higher concentrations resulted in the addition of glial injury. The results suggest that zinc should be included on the growing list of endogenous central neurotoxins which may be involved in the pathogenesis of CNS cell loss in a variety of disease states.

Animals↗

Neurons containing NADPH-diaphorase are selectively resistant to quinolinate toxicity.

Exposure of cultures of cortical cells from mouse to either of the endogenous excitatory neurotoxins quinolinate or glutamate resulted in widespread neuronal destruction; but only in the cultures exposed to quinolinate, an N-methyl-D-aspartate agonist, was there a striking preservation of the subpopulation of neurons containing the enzyme nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d). Further investigation revealed that neurons containing NADPH-d were also resistant to the toxicity of N-methyl-D-aspartate itself but were selectively vulnerable to the toxicity of either kainate or quisqualate. Thus, neurons containing NADPH-d may have an unusual distribution of receptors for excitatory amino acids, with a relative lack of N-methyl-D-aspartate receptors and a relative preponderance of kainate or quisqualate receptors. Since selective sparing of neurons containing NADPH-d is a hallmark of Huntington's disease, the results support the hypothesis that the disease may be caused by excess exposure to quinolinate or some other endogenous N-methyl-D-aspartate agonist.

Animals↗

Ketamine protects cultured neocortical neurons from hypoxic injury.

The general anesthetic ketamine, which has recently been reported to block the excitation of cortical neurons by N-methyl-D-aspartate (NMDA), was found to markedly reduce neuronal loss in murine neocortical cell cultures exposed to a hypoxic atmosphere or to cyanide. These observations may be relevant to attempts to find pharmacological means of minimizing hypoxic brain damage in the clinical setting.

Animals↗

Sporadic Creutzfeldt-Jakob disease in two coworkers.

We report the coincidence of pathologically confirmed sporadic CJD in two unrelated schoolteachers who shared a school wing for 9 months. The first developed ataxia, tremulousness, and dementia 5 months after his last contact with his colleague. Diagnosis of CJD was made 2 months later by brain biopsy. Eight months later, the second teacher developed similar symptoms and died after 9 months. Whether this unique coincidence reflects mere chance or some form of direct viral transmission is unknown. Continued epidemiologic surveillance for any future "coincidence" is warranted.

Brain↗

Glutamate neurotoxicity in cortical cell culture is calcium dependent.

Brief exposure to glutamate produced widespread neuronal death in mature, but not young, cortical cell cultures. Extracellular sodium replacement or addition of tetrodotoxin produced only minor reduction in this toxic neuronal loss. However, removal of extracellular calcium markedly reduced neuronal loss, and elevation of extracellular calcium accentuated neuronal loss. These observations suggest that the toxicity of glutamate on cortical neurons may depend primarily on the presence of extracellular Ca, probably through a mechanism which is distinct from simple 'excitotoxicity'.

Animals↗

Clonorchis sinensis: life cycle, intermediate hosts, transmission to man and geographical distribution in Korea.

The snail host of Clonorchis sinensis in Korea is referred to as Parafossarulus manchouricus, but it has been recorded also as Bulimus striatulus japonicus in literature published up to late 1950. The specific name of the snail was corrected such that Bulimus striatulus of China, Japan, and Korea should be incorporated in the species of P. manchouricus. In general, the snail exists in limited areas of the rivers Han, Kum, Mankyung, Naktong, and Yeongsan, and the infestation of the snail with the cercaria of C. sinensis is very low. In recent years, ten species of small fresh-water fish are known as second intermediate hosts of the fluke. Of these, the southern top-mouthed minnow, Pseudorasbora parva, was most heavily infected. The striped shiner, Pungtungia herzi, Korean shiner, Gnathopogon atromaculatus, gudgeon, Pseudogobio esocinus, oily shiner, Sarcocheilichthys sinensis, and Korean barbel, Hemibarbus labeo, were infected intermediately. Whereas, the flat bitterling, Paracheilognathus rhombea, oily bitterling, Acheilognathus limbata, and Korean rose bitterling, Acanthorhodeus taenianalis were infected with a few cysts. No infections were found in the pale chub, Zacco platypus, crussian carp, Carassius carassius, and carp, Cyprinus carpio. Most Koreans are usually infected by eating slices of raw fresh-water fish coated with hot bean paste. Raw fish is a common accompaniment in drinking the rice wine "Marcgulee" in rural communities and a health food for males. Numerous epidemiological studies revealed that the prevalence rate for the fluke among the residents in the vicinity of rivers is higher than those in the areas far from the rivers. The rate is higher among males than females and increases with age.

Adolescent↗

The ultrastructural characterization of the tegument of Clonorchis sinensis (Cobbold, 1875) cercaria.

The tegumental ultrastructure of the cercaria of the liver fluke, Clonorchis sinensis (Cobbold, 1875), was studied by scanning and transmission electron microscopy. The body surface is almost encircled by many rows of regularly arranged spines. The tegumental syncytium of the body contains many rod-shaped dense granules and central electron-lucent bodies, neither of which are present in the tail tegument. There are four rows of hooked teeth and modified spines on the oral cone. These teeth are differentiated morphologically and probably functionally from the other body spines. Disc-shaped papillae with long or short cilia are distributed on the body in a bilaterally symmetrical pattern dorsally and ventrally. There are 30 to 37 papillae with much longer cilia laterally. Four pairs of papillae were found on the tail. From their structure and location these papillae appear to have a mainly tango- or rheoreceptive function. Another type of sheathed papillae is situated around the oral sucker. The cuticular tegument is expanded laterally at the base of the tail, forming a characteristic sac-like structure.

Animals↗

Non-NMDA receptor-mediated neuronal injury in Alzheimer's disease?

The foregoing review by J. T. Greenamyre and A. B. Young provides an excellent discussion of the alterations in excitatory amino acid transmitter systems that accompany Alzheimer's disease, and the interesting possibility that excitotoxicity may participate in disease pathogenesis. I would add a speculation that non-NMDA receptors may be particularly important in mediating such excitotoxicity.

Alzheimer Disease↗