PubMed Health⌕ Search

Biomedical subjects

D W Qiu

Publications and source records attributed to D W Qiu.

2 recordsLinked to original sources
PubMed1997

[Effect of migao xinle droplet pills on free radicals and serum nitric oxide on myocardial ischemia reperfusion injury in rats].

OBJECTIVE: To observe the protective effects of Migao Xinle Droplet Pills (MXLDP) on experimental myocardial injury induced by ischemia and reperfusion in rat on the basis of the cytoprotection theory. METHODS: Studied the clearance capacity of MXLDP to oxygen free radicals and the contents of serum nitric oxide (NO) on the myocardial ischemia reperfusion injury model was produced by the 45 min ligation of left descending coronary artery and reperfusion in rats. RESULTS: MXLDP pretreatment (30 and 120 mg.kg-1.d-1) prevented significantly reduction of myocardial superoxide dismutase (SOD) activity, reduced malondialdehyde (MDA) content and myocardial creatine phosphokinase (CPK) release; diminished significantly myocardial infarction size, decreased lactate dehydrogenase (LDH) activity and increased the concentration of serum NO in comparison with the control (P < 0.05-0.01). CONCLUSIONS: It has a protective effect on myocardial ischemia/reperfusion injury. The action is considered to be related to the inhibition of the oxygen free radicals and subsequent lipid peroxidation as well as prevented reduction of NO.

Animals↗
PubMed1996

Simplified method for measuring urinary leukotriene E4.

The conventional method for measuring urinary leukotriene E4 (LTE4) is by reversed-phase high-performance liquid chromatography (RP-HPLC), followed by radioimmunoassay (RIA) or enzyme immunoassay (EIA). We measured urinary LTE4 levels by two methods, HPLC with EIA and EIA alone after initial crude extraction of urine using an octadecyl reversed-phase extraction cartridge (Sep-Pak). Ninety-three urine samples from normal subjects and patients with bronchial asthma and adult respiratory distress syndrome were tested. The results showed that urinary LTE4 levels measured by EIA significantly correlated with those measured by HPLC plus EIA in the three groups (r = 0.88, 0.85, 0.68). The absolute values of urinary LTE4 measured by EIA without HPLC purification were higher than by EIA with HPLC purification. This suggests that HPLC may not be necessary for routine urinary LTE4 quantitation in different clinical situations.

Adult↗