Biomedical subjects

D Whitaker

Publications and source records attributed to D Whitaker.

At least 37 records · Page 2Linked to original sources

PubMed1999

Non-veridical size perception of expanding and contracting objects.

Observers were presented with various types of stimulus expansion and contraction which resulted in marked misperceptions of size. Firstly, the perceived size of an object which is changing in size is shown to be biased in the direction of the size change. Secondly, expansion or contraction of the internal texture of objects is found to influence their perceived size. Finally, an illusory texture manipulation in the form of a movement after-effect is shown to produce the same type of size misperception as a real expansion or contraction of internal texture. The spatio-temporal characteristics of these illusory size changes are investigated.

Humans↗

PubMed1999

Vernier acuity with compound gratings: the whole is equal to the better of its parts.

To evaluate the relative importance of local feature and spatial filter models for hyperacuity, vernier thresholds were determined for gratings consisting of a fundamental (Fo) and third harmonic (3F) presented alone, and added together in compound gratings where the relative phase offsets of 3F to Fo was 0, 90, 180 and 270 degrees. Thresholds were determined for a range of spatial frequencies of Fo (0.5-16 c deg-1) for abutting and non-abutting stimuli. Compound grating vernier performance was found to be: (i) invariant with relative phase offset for the abutting and non-abutting conditions; and (ii) predictable from the vernier thresholds for the individual grating components making up the compound stimulus. The results support a view that supra-threshold components in a multi-frequency stimulus act independently and it is the spatial frequency content, not the local feature characteristics, which limit vernier performance.

Humans↗

PubMed1999

Discriminating mirror symmetry in foveal and extra-foveal vision.

The ability to discriminate perfect from imperfect mirror symmetry was examined at the fovea and at eccentricities out to 10 degrees in the nasal visual field. A 2-AFC method of constant stimuli was employed in which a bilaterally symmetric pattern was presented in one interval and a degraded version of this symmetric pattern in the other. The subject's task was to decide which interval contained the perfectly symmetric pattern. Pattern size was varied by changing the viewing distance. Probit analysis revealed the degree of asymmetry corresponding to 75% correct performance. Given sufficient size scaling, perfectly symmetric stimuli can be discriminated from degraded symmetric stimuli in extra-foveal vision. Spatial scaling with an E2 value similar to that for positional acuity was successful in removing the eccentricity dependence for the task.

Fovea Centralis↗

PubMed1999

Spatial characteristics of the second-order visual pathway revealed by positional adaptation.

The visual system is thought to process luminance (first-order) and contrast (second-order) information by dedicated cortical streams. To explore the spatial characteristics of the second-order pathway, we examined the effect of adaptation on spatial localization in human subjects. We show that, unlike first-order adaptation, second-order positional adaptation via cortical mechanisms transfers across orientations but not across spatial frequencies. These results support physiological evidence that these two processing streams are distinct and suggest that the cortical mechanism mediating second-order positional adaptation maintains spatial frequency information but sums signals across orientations.

Adaptation, Ocular↗

PubMed1999

A diversified patient education program for transplant recipients.

Patients who receive a solid organ transplant not only have many learning needs, but also lifestyle changes related to the transplant. Previous experiences with health care, beliefs about health, language and reading skills, cultural influences, education preparation, and disabilities are a few of the factors that influence how each patient learns posttransplantation, and adjusts to a new lifestyle. Staff nurses on our transplant unit have taken these factors into consideration when developing various concrete teaching tools that include a book, medication card, color-coded medication guide, audiocassette, computer printouts, and clinical pathways. Because each patient comes with a unique story, approaches for patient learning are individualized by the nursing staff. The use of the tools, combined with an individualized and nonjudgmental approach, has created a caring environment for patient learning.

Adaptation, Psychological↗

PubMed1998

Medullary carcinoma of the thyroid: accuracy of diagnosis of fine-needle aspiration cytology.

BACKGROUND: A preoperative diagnosis of medullary carcinoma of the thyroid (MCT) allows for the investigation of associated multiple endocrine neoplasia/ pheochromocytoma, and definitive surgery without the need for frozen section. Criteria for cytodiagnosis are well known but variable patterns of presentation may cause diagnostic difficulty. METHODS: This study examines the accuracy of cytodiagnosis and the value of ancillary tests in 17 patients seen between 1976 and 1997. Nine patients underwent thyroid gland aspirations, five patients underwent fine-needle aspiration (FNA) of the thyroid and cervical lymph nodes, and three patients underwent cervical lymph node aspiration alone. Electron microscopy (EM) of aspirated material was performed in nine cases and immunocytochemistry in two cases. RESULTS: In all cases the diagnosis was suggested by FNA. In four cases, diagnosis and management were based on cytology alone. EM of FNA material was confirmatory in nine cases, two of which also showed positive calcitonin immunocytochemistry. In three cases the diagnosis was not proven until surgical resection, and in one case FNA confirmed lymph node metastasis in known MCT. Frozen section in five patients did not change the level of diagnostic confidence over the FNA diagnosis in any case. In four other thyroid tumors (one Hürthle cell follicular carcinoma, two anaplastic carcinomas, and one hyperplastic nodule) MCT was suspected in the FNA differential diagnosis but later excluded. In the Hürthle cell tumor immunoperoxidase staining was positive for calcitonin and in one anaplastic carcinoma, a neuroendocrine phenotype was suggested. In the latter case, additional EM excluded MCT. CONCLUSIONS: Although correct diagnosis is made by cytology in the majority of instances, other tumors may show cytologic findings similar to MCT. EM of FNA material was found to be the most definitive method of proving or excluding MCT. Immunocytochemistry may be misleading for rarely performed tests.

Adolescent↗

PubMed1998

The use of epithelial membrane antigen and silver-stained nucleolar organizer regions testing in the differential diagnosis of mesothelioma from benign reactive mesothelioses.

BACKGROUND: The accurate diagnosis of pleural lesions obtained from small closed biopsy is difficult. As yet there is no single reliable test to distinguish between malignant and benign mesothelial tissue. METHODS: Immunostaining of epithelial membrane antigen (EMA) and the quantitation of silver stained nucleolar organizer regions (AgNORs) each were applied to benign and malignant histologic sections of pleural and peritoneal biopsies. The usefulness of these stains was tested both individually and in combination in the diagnosis of epithelial malignant mesothelioma. RESULTS: One hundred and three of the 141 malignant lesions (73%) were immunoreactive for EMA but only 3 of the 73 benign lesions (4%) reacted equivocally, and none positively. The average count of AgNORs/cell in malignant lesions (n = 80) was elevated compared with benign cases (n = 26), but a significant overlap was exhibited in the AgNOR count and this form of analysis was considered to be of little value in distinguishing benign from malignant mesothelial processes. Much less overlap was observed when the average AgNOR area was measured. By using the maximum benign AgNOR area of 0.6677 microm2 as the upper threshold, 51 cases (63.8%) were identified as malignant; the test demonstrated 100% specificity and 63.8% sensitivity. By combining the EMA and AgNOR results, 76 of 80 of the malignant mesothelioma cases (95%) tested positive for at least 1 of the tests with no false-positive results identified. CONCLUSIONS: This study confirms the usefulness of EMA in diagnosing malignant and benign mesothelial lesions, and demonstrates the enhanced diagnostic value of combining EMA immunoreaction with the average area of AgNOR per cell, thereby increasing sensitivity in the diagnosis of epithelial malignant mesothelioma.

Adolescent↗

PubMed1998

The effect of suprathreshold contrast on stimulus centroid and its implications for the perceived location of objects.

Using an alignment task, we investigate the role of suprathreshold contrast upon the perceived location of asymmetric Gaussian-windowed stimuli. A model which extracts the centroid of the stimulus envelope between limits defined by contrast threshold accounts well for the observed variation in perceived position. This finding helps to explain previous discrepancies in the literature regarding the validity of stimulus centroid as a determinant of visual location.

Contrast Sensitivity↗

PubMed1998

Geometric representation of the mechanisms underlying human curvature detection.

Combined manipulation of blur, line length and contrast reveal two distinct processes involved in curvature detection. When line length is small relative to blur, thresholds are almost directly proportional to blur and independent of line length. When line length is large relative to blur thresholds are directly proportional to line length and independent of blur. The aspect ratio (line length/blur) of curved contours represents a scale-invariant metric which forms the decisive factor in determining curvature performance.

Contrast Sensitivity↗

PubMed1998

Reactive mesothelial hyperplasia vs mesothelioma, including mesothelioma in situ: a brief review.

In biopsy tissue, discrimination between reactive mesothelial hyperplasia and epithelial mesothelioma can pose a major problem for the surgical pathologist. Confidence in the diagnosis is often proportional to the amount of tissue available for study and depends largely on findings of invasion and the extent and cytologic atypia of the lesion, because there is no marker specific for the mesothelium and that discriminates consistently among normal, hyperplastic, and neoplastic mesothelial tissue. Therefore, mesothelioma in situ is diagnosable only when invasive epithelial mesothelioma is demonstrable in the same specimen, in a follow-up biopsy specimen, or at autopsy. Comparison of 22 cases of mesothelioma in situ that fulfill these requirements for diagnosis with 141 invasive mesotheliomas and 78 reactive mesothelioses indicates that strong linear membrane-related labeling for epithelial membrane antigen and silver-labeled nucleolar organizer region-positive material that occupies 0.6677 microm2 or more of the nucleus in an atypical in situ mesothelial lesion of the pleura are found consistently in neoplastic mesothelial cells. Although these findings may engender suspicion of mesothelioma in situ in high-risk persons, the criteria for diagnosis of pure mesothelial lesions of this type are still under study. Mesothelioma in situ should be considered proved only when unequivocal invasion is identified in a different area of the pleura or at a different time; a diagnosis of pure mesothelioma in situ should not be made in patients not exposed to asbestos.

Biopsy↗

PubMed1998

Positional acuity in amblyopia: does a perceptual consequence of neural recruitment exist?

Animal models of amblyopia have shown that visual deprivation for even brief periods can result in dramatic changes in cortical architecture. Active neural recruitment mechanisms present the possibility that the non-deprived eye of amblyopes may show enhanced visual capacity. This idea was tested by measuring a form of positional acuity which we have termed alignment threshold. Three subject groups were examined, adults, visually normal children, and children with amblyopia in which the non-deprived eye was tested. Alignment thresholds in adults were significantly better (approximately 0.3 log unit) than the thresholds for visually normal children. No significant difference was found in thresholds between the visually normal children and the non-deprived eye of the amblyopic children. The results of this study suggest that subjects with unilateral amblyopia do not show enhanced visual alignment performance in their non-deprived eye.

Adult↗

PubMed1998

Early detection of changes in visual function in diabetes mellitus.

Psychophysical measurements were performed with a view to providing an assessment of early neural functional integrity in the presence of no or minimal diabetic retinopathy (DR). The investigations were carried out on a normal group and a number of age- and sex-matched non-insulin dependent diabetic groups with different levels of DR. Results were considered in terms of the presence of functional changes relative to the severity of DR and the duration of diabetes. Significant deficits were found for visual acuity (VA), contrast sensitivity (CS) and colour vision. Both CS and colour vision measurements differentiated between non-diabetics and those with diabetes but without DR. However, none of the tests reliably distinguished diabetics without DR from those with early retinal changes although VA and colour vision (especially along a blue-yellow colour axis) were sensitive to more substantial retinal changes. This finding suggests such tests may be useful as screening tests for more advanced levels of background DR.

Adult↗

PubMed1997

Disentangling the role of spatial scale, separation and eccentricity in Weber's law for position.

Factors underlying Weber's law for position were investigated by measuring spatial interval discrimination accuracy for spectrally narrow-band stimuli. These stimuli were positioned around an iso-eccentric arc in order to allow separation and eccentricity to be varied independently. We find that Weber's law occurs at individual spatial scales, and holds true not just for stimuli positioned either side of fixation, but for any series of stimuli which possess the same ratio of separation to eccentricity. When the separation/eccentricity ratio is large, thresholds are proportional to eccentricity and demonstrate contrast independence. At smaller separation/ eccentricity ratios, thresholds are determined by a contrast-dependent combination of separation and eccentricity.

Contrast Sensitivity↗

PubMed1997

The effects of eccentricity and stimulus magnification on simultaneous performance in position and movement acuity tasks.

We presented two tasks, spatial interval discrimination and displacement detection, simultaneously in the same location at various eccentricities. The subject was to solve (i) only the spatial interval task; (ii) only the displacement task; or (iii) both tasks simultaneously. With 500 msec stimulus duration, and using the method of spatial scaling, the E2 value (the eccentricity at which stimulus size has to be doubled to maintain performance level) was found to be 0.17-0.39 deg for spatial interval discrimination and 1.0-1.2 deg for displacement detection. These values remained unaffected whether the subject solved one task or two tasks simultaneously. This finding was confirmed using a shorter, 50 msec stimulus duration. As there is no interference between tasks, the mechanisms solving the tasks appear to be functionally independent i.e., operating in parallel at all eccentricities.

Humans↗

PubMed1997

The influence of adaptation on perceived visual location.

We demonstrate a marked effect of prior adaptation upon the perceived position of subsequently presented stimuli using both first-order (luminance-defined) and second-order (texture-defined) stimuli. The effect of varying the contrast of the adapting and test stimuli depends only upon the ratio of adapting/test contrast. Adaptation effects for the two types of stimuli differ in terms of interocular transfer and rate of decay. Whilst adapting and testing with the same type of stimulus (first- or second-order) produces large shifts in perceived position, little or no crossover effect was found. The data are accounted for by a model in which the centroid of the linear combination of after-image and test stimulus is extracted.

Adaptation, Ocular↗

PubMed1997

Cytokine regulation of mesothelial cell proliferation in vitro and in vivo.

Previous studies have demonstrated mitogenic effects of several mediators on mesothelial cells in vitro, but their effects in vivo have not been investigated. The aim of this study was to examine the effects of various cytokines on normal mesothelial cell proliferation in vitro and in vivo and correlate the findings in both assay systems. In vitro proliferation was assessed using a technique based on the uptake and subsequent release of methylene blue. Autoradiographic methods were applied in a murine model to assess mitogenic activity of these factors on mesothelium in vivo. In vitro data demonstrated a dose-dependent increase in human mesothelial cell proliferation by all mediators examined: at optimal concentrations, proliferation was enhanced between 26.53 +/- 3.77% standard deviation (SD), p < 0.001 for fibroblast growth factor-2 (FGF-2) and 114.58 +/- 6.97%, p < 0.001 for platelet-derived growth factor-AB (PDGF-AB) above control medium. In vivo, DNA synthesis in mesothelial cells was stimulated by FGF-2 (29.52 +/- 5.85% labeled cells, compared with 7.04 +/- 4.36% for control medium; p < 0.001), tumor necrosis factor-alpha (TNF-alpha; 13.14 +/- 4.55% compared with 7.23 +/- 2.85; p < 0.005) and PDGF-BB (11.53 +/- 4.74% compared with 4.67 +/- 3.48%; p < 0.005). Transforming growth factor-beta1 (TGF-beta1) and epidermal growth factor (EGF) had no effect on DNA synthesis in mesothelial cells in vivo. It is concluded that FGF2, TNF-alpha, and PDGF stimulate mesothelial cell proliferation in vitro and in vivo, whereas TGF-beta1 and EGF only had a mitogenic effect in vitro at the concentrations examined. The mitogenic potency of the different PDGF isoforms in vitro was consistent with PDGF-alpha and beta receptor expression.

Animals↗

PubMed1997

Detection of geometric image distortions at various eccentricities.

PURPOSE: Human ability to perceive spatial stimuli declines with increasing eccentricity. To study this phenomenon with natural images, the authors applied the spatial scaling method by measuring the smallest detectable amount of geometric change in a human face at several eccentricities for a series of stimulus magnifications to find out whether performance could be made equal across the visual field simply by an appropriate enlargement. METHODS: The authors used a novel method to produce subtle changes to an image of a face. The smallest change recognized was determined using a two-alternative forced-choice method and expressed in terms of correlation sensitivity, the inverse of the correlation between the images that just could be discriminated. RESULTS: The detection of changes in the facial features, presumably a spatially complex task, became equal across the visual field simply by an appropriate change of scale. The E2 value represents the eccentricity at which the foveal stimulus size must double to maintain performance at the foveal level. The E2 values, found to be 1.73 degrees to 2.45 degrees, were similar to our previously measured values for vernier acuity, orientation discrimination, and curvature detection and discrimination, obtained with the same method of spatial scaling. CONCLUSIONS: The authors' results indicate that with adequate stimulus magnification, one is capable of detecting geometric changes in complex images such as face equally at the fovea and in the periphery. In this task, there seems to be no qualitative difference between the accuracy of foveal and peripheral processing.

Face↗

PubMed1996

Changes in the concentration of microvilli on the free surface of healing mesothelium are associated with alterations in surface membrane charge.

The luminal plasmalemma of regenerating mesothelial cells was examined by transmission electron microscopy and the concentration of microvilli at various stages of healing was quantified. Charged tracer and lectin binding techniques were also employed to investigate electrostatic and chemical changes in mesothelial glycocalyx. In uninjured mesothelium and at all stages of healing, the concentration of microvilli at the cellular periphery was greater than over the main cell mass (P < 0.05). Furthermore, there was an increase in the concentration of microvilli in all regions by day 4, which reached a maximum at day 6, then at days 10-15 returned to values closer to uninjured mesothelium (P < 0.01). These changes were associated with an alteration in surface charge. In all lesions, the surface charge on microvillar membranes was greater than for flat membranes, but the difference was only significant at days 4, 6, and 15 (P < 0.001). The changes in surface charge may reflect a differential expression of mucopolysaccharides on the surface membrane. In addition, concanavalin A bound avidly to mesothelial surface membranes, suggesting the presence of alpha-methyl-D-mannoside residues. These findings suggest an association between microvillar formation and surface charge, the former protecting the healing mesothelium by enhancing entrapment of serosal fluid and its contents.

Animals↗