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D Wrońska

Publications and source records attributed to D Wrońska.

7 recordsLinked to original sources
PubMed1998

Vaccinia virus-induced changes in cytokine-regulated acute phase plasma protein synthesis by hepatoma cells.

Human hepatoma cell line, HepG2, has been infected with vaccinia virus and synthesis of plasma proteins was determined by electroimmunoassay and corresponding mRNA's measured by Northern blotting. The inhibitory effect of the virus was dose- and time-dependent. Electrophoretic mobility shift assay revealed a decrease in C/EBP binding activities in nuclear extracts isolated from the infected hepatoma cells. Supershift analysis of the C/EBP isoforms showed alpha and beta subunit involvement in DNA binding. The treatment of the cells with interleukin-1, interleukin-6, and dexamethasone at the initial stage of infection appears to delay the virally induced inhibition of host cell protein synthesis. Thus, possible "protective" role of the acute phase cytokines in viral infection is proposed.

Acute-Phase Proteins↗
PubMed1993

Induction of DNA synthesis by microtubule depolymerization is mediated by actin filaments.

Colcemid (0.4 microgram/ml) induced DNA synthesis in quiescent chick embryo fibroblasts, and increased the amount of F-actin. Cytochalasin D, which disorganized actin filaments, inhibited the stimulation of DNA synthesis by colcemid. These results suggest that actin filaments are involved in the sequence of events leading from the depolymerization of microtubules to the initiation of DNA synthesis. They also demonstrate that depolymerization of microtubules can influence the amount of F-actin in the cell.

Actins↗
PubMed1991

[Effect of phenothiazine derivative on adrenal cortex response of sheep to repeated emotional stress].

The study was aimed at the evaluation of propiopromazine (Combelen, Bayer), a derivative of phenothiazine, as an agent lowering in sheep the response to stress. The stress of emotional origin was induced in sheep by the isolation from herd lasting 1 hour. The isolation experiments were repeated 6 times on the same group of sheep, first three isolations (1-3) in daily intervals and next three (4-6) in weekly intervals. Propiopromazine was administered before each isolation experiment. The reaction of sheep to the isolation stress was weaker after propiopromazine administration. This was suggested by smaller increase in blood serum cortisol and glucose levels when compared to sheep subjected to isolation but not receiving the drug. Such effect was especially conspicuous during the course of the first isolation experiment; during the next experiments the difference concerning the reaction to stress between the sheep isolated from the herd receiving and not receiving the drug was gradually diminishing. It was shown in addition that propiopromazine administration to the sheep not subjected to stress caused an increase in cortisol level by 125 per cent and that in glucose level by 35 per cent. These results suggest that propiopromazine administration protects the organism against the effects of emotional stress only partially. Moreover, the effect of its administration gradually weakens with repeating of the stress inducing experiment, and propiopromazine itself may act as a stress inducing factor. It seems therefore that the use of propiopromazine and similar compounds as anti-stress agents may be questionable.

Adrenal Cortex↗