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Biomedical subjects

D X Huang

Publications and source records attributed to D X Huang.

At least 19 recordsLinked to original sources
PubMed2001

[Recent progress of study on single nucleotide polymorphisms].

Single nucleotide polymorphisms (SNPs) is a new genetic marker system following RFLP and microsatellite polymorphism. It has been shown to have the characteristics of high-density, inheritance stability and facilitation in analysis automation. SNPs can be detected by electrophoresis, endonuclease-cleavage, PCR and sequencing, and can be used extensively in gene mapping, disease-correlativity analysis, population genetics and drug research.

Chromosome Mapping↗
PubMed2000

[Inhibition of ERK1/2 activity and c-fos mRNA after coronary artery balloon injury by intracoronary radiation in swine].

The effect of intracoronary radiation on extracellular signal regulated kinase 1/2 (ERK1/2) activity and c-fos mRNA after coronary artery balloon injury was investigated in swine. Twenty three swines were randomly divided into a radiation group and a control group after coronary balloon over stretch. The dilated segments in the radiation group were exposed to a dose of 20 Gy by a catheter based radiation system. The animals were sacrificed at 3 (6 swines from each group) and 30 days (6 swines from radiation group and 5 from control group) after the operation. The injured segments were processed to examine c-fos gene expression by reverse transcription polymerase chain reaction (RT PCR) and to measure the activity of ERK1/2 biochemically. Intracoronary radiation decreased significantly the ERK1/2 activity and gene expression of c-fos in the radiation treated animals 3 days after coronary balloon injury (20.5%,P<0.01; 47.7%,P<0.05), but neither ERK1/2 activity nor c-fos gene expression was significantly affected by endovascular radiation in animals 30 days after balloon injury. Therefore, both ERK1/2 and c-fos may be involved in inhibiting restenosis.

Angioplasty, Balloon, Coronary↗
PubMed1993

Normal gestation after 5 years on home parenteral nutrition.

A case of normal gestation after 5 years on HPN is reported. The patient had her entire jejunum and ileum and right colon resected because of gangrene and has subsisted normally on HPN ever since. From the second trimester on, her HPN formula was supplemented to meet the requirement for growth and development of the foetus. Anaemia, hypoalbuminaemia, low plasma zinc levels and jaundice became manifest in the final trimester. These responded readily to readjustment of her HPN formula. A normal baby weighing 2020 g was delivered by Caesarean section at 36 weeks. Long-term HPN, final trimester abnormalities and HPN formula adjustments are discussed.

Journal Article↗
PubMed1993

[Experiences in improving prognosis of acute necrotizing pancreatitis].

Seventy-eight cases of acute necrotizing pancreatitis were analysed. Mortality varied with: timing of operation (14.3% after 5 days vs. 37.9% within 5 days), procedure (16.7% after necrosectomy vs. 44.4% after partial pancreatectomy) and nutritional support (15.0% with nutritional support vs. 50.0% without). Univariate (Chi-square test) and multivariate analyses (Logistic regression analysis showed correlation between each variable (timing of operation, procedure and nutritional support) and survival. It is felt that delayed necrosectomy with intraabdominal lavage, and early nutritional support may help to improve prognosis of this disease.

Acute Disease↗
PubMed1993

Blood pressure and life style in the People's Republic of China: three samples in the INTERSALT Study.

In INTERSALT, the International Cooperative Study on Electrolytes and Blood Pressure, three centres located in the People's Republic of China (PRC) had distinctive patterns of BP and of life style variables that, in INTERSALT overall, were found to relate to BP. The PRC centres had low body mass index (BMI) and alcohol consumption but high urinary sodium and sodium/potassium ratio (Na/K). Compared with 45 other INTERSALT centres, average BMI was 22.7 vs. 25.4 and alcohol consumption was 32% vs. 61%, with heavy drinking 3% vs. 14%. However, Na/K in the PRC was 6.7 vs. 3.2 in the other centres. This combination of factors may underlie the BP pattern observed. While mean BP in the PRC was lower than in the 45 centres (-7.0 mmHg SBP, -5.6 mmHg for DBP), this was counterbalanced by other findings. Upward slope of systolic pressure with age was 45% greater for the PRC than the other centres and 24% greater for diastolic pressure. As a result, although percentage hypertensive for ages 20-49 years for the PRC was half that in the 45 centres (6% vs. 12%), by age 50-59 years the prevalence was 31% in the PRC and 38% in the other centres. Hypertension prevalence in Tianjin, with highest Na/K in INTERSALT (7.6) was 40% for ages 50-59 years. While sample size in individual centres does not provide power to demonstrate aetiological relationships, these findings permit formulation of the following hypothesis: although lower body mass and lower alcohol intake may counteract to some degree impact of high salt intake, such intake over decades eventually takes its toll on BP.

Adult↗
PubMed1992

The all-in-one nutrient solution in parenteral nutrition.

Dextrose, lipid emulsion, amino-acids, electrolytes, vitamins and trace elements were mixed in a well defined order to prepare 10 different total nutrient admixture solutions. The pH and osmolality of these solutions did not change significantly during 14 days of storage at 4 degrees C. Mean diameters of lipid particles on different occasions for the different solutions varied from 0.31 +/- 0.10 mum to 0.48 +/- 19 mum with 95% of particles less than 0.6 mum. Particles greater than 6 mum were not observed. Cultures were all negative. 243 patients received 5101 infusions of the admixture fluids. Patients on treatment for more than 1 week showed improved nitrogen balance. Serum transferrin and albumin were increased when treatment was ongoing for more than 2 weeks. No adverse reactions or abnormal laboratory findings were observed. One patient, with only duodenum and a part the colon left, has been maintained in good health for more than 55 months using this kind of nutritional support.

Journal Article↗
PubMed1992

Defibrase, a purified fibrinolytic protease from snake venom in acute myocardial infarction.

To investigate the thrombolytic effects of defibrase in AMI, 157 pts with AMI were studied. Of the 157 pts, 87 were assigned to defibrase thrombolysis and 70 to the conventional therapy plus heparin (control). Coronary arteriography was performed in 36 pts of the defibrase group and 26 of the control group. Pretreatment coronary arteriography was performed in 10 pts and total occlusion of infarct-related artery (IRA) was demonstrated in 7. Thirty to 45 min after either intracoronary or intravenous defibrase thrombolysis, recanalization occurred in 4 of the 7 pts. The perfusion and stenosis was improved in 2 of the 3 pts with patent IRA. Of the 10 pts who underwent emergency coronary arteriography after the onset of i.v. defibrase thrombolysis, 6 were shown to have patent IRAs. Of the 16 pts who underwent coronary arteriography two weeks after the i.v. defibrase thrombolysis, 13 were shown to have patent IRAs. In contrast, only 11 of the 26 pts in the control group had patent IRAs two weeks after admission to the hospital. Of the 36 angiographic cases of the defibrase group, 15 underwent follow-up coronary arteriography, only 1 pt showed reocclusion. In comparison to the control group, the pts in the defibrase group had earlier CPK peaking, higher percentage of pts with LVEF > 0.5, a lower mortality and complication rate. Major spontaneous bleeding complications were rarely seen with defibrase. The results indicate that defibrase is an effective thrombolytic agent with a reasonable recanalization rate, low reocclusion rate and a low rate of bleeding complications.

Batroxobin↗
PubMed1991

[The prehospital emergency cardiac care in patients with acute myocardial infarction].

In order to evaluate the current status of prehospital care of acute myocardial infarction (AMI) in this city, the clinical manifestations and the emergency cardiac care in prehospital phase in 210 patients were reviewed in the period from 1987 to 1988. Before admission to CCU, 45.7% of the patients had complications of severe cardiac arrhythmias or hemodynamic disturbances and 14.8% had cardiac arrests. The mortality during the first 4 weeks was 25.2%. Among 53 patients who died, 50.9% occurred before admission and 37.7% within 1 hour. One hundred and ten patients (52.4%) had prehospital care and, 4 of them provided by the City Emergency Center and others by the local clinics near by. The median time from the cardiac attack to the first medical care, hospital arrival and CCU admission was 2, 3.6 and 6.3 hours respectively. The management on the scene was not efficient and effective in many patients. The rate of successful resuscitation in patients who had cardiac arrest out of hospital was only 7.7%. We are impressed that the mortality of patients with AMI is mainly in the early stage after appearance of symptoms. The initiation of emergency cardiac care is very necessary. The current status of prehospital cardiac care needs to be improved immediately, some suggestions are made for this purpose.

Aged↗
PubMed1991

Ketanserin in the treatment of hypertension: a multicentre dose-finding study in China.

The effect of ketanserin at 5 mg, 10 mg and 20 mg twice daily for 3 months was studied in Chinese patients with hypertension. Both 10 mg and 20 mg doses twice daily effectively reduced systolic and diastolic pressures, while 5 mg twice daily was not effective. The 20 mg regimen was more effective than 10 mg in reducing diastolic, but not systolic pressures. Blood pressure reduction was progressive up to 1 month of treatment but not thereafter. Neither first-dose hypotension nor postural hypotension were seen. There were no effects on body weight or heart rate. A nominally significant 6 msec increase in QTc was seen with 10 mg, but not with 5 or 20 mg twice daily; this could be a chance finding. The side-effect burden was light, and decreased with time.

Blood Pressure↗
PubMed1990

Serum lipid and apolipoprotein levels in a Tibetan population sample.

Serum lipid and apolipoprotein levels were measured in a Tibetan population sample. In both men and women serum cholesterol levels were markedly lower than in Western populations. In men HDL-cholesterol levels were higher compared to both Oriental and Western populations while in women the values were markedly higher than in other Oriental populations and similar to Western populations. Highly significant positive relationships were found between serum lipid levels and corresponding apolipoprotein levels. In men but not in women serum cholesterol was markedly higher in office workers than in factory workers.

Adult↗
PubMed1989

[Cyclic AMP levels in the plasma and ischemic myocardium and free fatty-acid levels in the serum following coronary artery ligation in dogs: relation to ventricular fibrillation].

This study was designed to shed light on the biochemical causes of serious ventricular arrhythmias in the early stage of acute myocardial infarction (AMI). Fourteen mongrel dogs were divided into 2 groups. One group was subjected to coronary ligation by placing silk sutures around the LAD coronary artery about 1.5 cm from its origin. All the dogs of this group were found to have ventricular tachycardia and fibrillation, starting approximately 18 min. After ligation, associated with regional accumulation of myocardial cAMP in the ischaemic zone. The increase of cAMP started about 10-15 min. before the onset of the arrhythmia. In contrast, the control group had only sutures placed without ligation and was not found to have change in myocardial cAMP within 35 min and arrhythmias at the same time. Both plasma cAMP and serum FFA were studied, but no significant changes were found. It is concluded that myocardial cAMP may play an important role in the genesis of ventricular arrhythmias in the acute ischaemic heart and it may be one of the arrhythmogenic factors to evoke ventricular arrhythmias.

Animals↗
PubMed1989

[Hemodynamic study of a single oral dose of enalapril in patients with heart failure].

Clinical and hemodynamic studies were carried out for the purpose of evaluating the effects of enalapril in the treatment of chronic heart failure. Enalapril 10-20 mg was given once to 10 patients with moderate to severe congestive heart failure (coronary 6, hypertensive heart disease 2 and idiopathic congestive cardiomyopathy). Hemodynamic studies were done on the first day after drug administration and the same dose was maintained for 2 weeks. The results showed a decrease of mean BP of 18.29% (P less than 0.01), CVP 40.4% mPAP 23.9%, PCWP 41.2%, SVR 39.9% and PVR 41.3% respectively (P less than 0.001). Cardiac index increased 44.16% (P less than 0.001). The therapeutic action lasted more than 24 hours after a single dose. No further drop of BP was found weeks later, 6 patients in NYHA class III and 2 in class IV improved to class II. No severe side effects were found. The results showed that enalapril is well tolerated after a single dose and its therapeutic action lasts 2 weeks with improvement both in hemodynamics and subjective symptoms.

Administration, Oral↗
PubMed1989

[Studies on antifertility constituents of Siegesbeckia glabrescens Mak].

Three diterpenes including a new compound were isolated from the whole plant of Siegesbeckia glabrescens Mak. The new one was named as neodarutoside and its structure was elucidated as ent-3 alpha, 15,16-trihydroxy pimarane 3,15-bis-(beta-glucopyranoside) based on the spectral evidence and chemical transformation. The other two were darutigenol and darutoside. Darutoside possesses the activity of termination of early pregnancy in experimental rats at a dosage of 20-40 mg/kg.

Abortifacient Agents, Nonsteroidal↗
PubMed1988

A preliminary study on the therapeutic effects of a calcium antagonist (diltiazem) on primary congestive cardiomyopathy. A clinical survey of 15 cases.

The clinical effects of diltiazem in 15 patients with congestive cardiomyopathy were observed. Symptoms were controlled in 66% and cardiac enlargement improved in 73.3%. There was a decrease in cardiac silhouette on X-ray in 18.82 +/- 3.2% (p less than 0.01), with an increase of EF(%), CO, CI and a decrease of TPR on two-dimensional (2-D) echocardiogram (p less than 0.01). Prior to treatment, the cardiac status of 8 cases was grade III and 7 cases grade IV; after treatment 1 case was grade I, 8 grade II, 5 grade III and 1 grade IV. This improvement might be due to the blockade of calcium channels and the fact that diltiazem has the least negative inotropic effect among the calcium antagonists. The dosage, route of administration, and prevention of side effects are discussed.

Adolescent↗