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Biomedical subjects

D Z Huang

Publications and source records attributed to D Z Huang.

8 recordsLinked to original sources
PubMed1996

[The antibacterial efficacy to obligate anaerobes isolated from the infected root canal of deciduous teeth]

TMJ osteoarthrosis(OA) is frequently seen in the Oral and Maxillofacial Surgery clinics.In order to investigate cellular and molecular basis of the pathogenesis of TMJOA,the levels of IL-1 and IL-6 in synovial aspirates from different stages of TMJOA patients were determined and analysed.It was found that both of them were highly correlated with the severity of TMJOA(P<0.0001).A positive linear correlation was also found between IL-1 and IL-6(P<0.0001).The balance and imbalance between MMPs and TIMPS and the modulatory mechanism of cytokines were introduced and their possible roles in the pathogenesis of TMJOA were discussed.

Journal Article↗
PubMed1995

Measures of vocal function during changes in vocal effort level.

The purpose of this article is to present the results of a controlled study of the day-to-day variabilities of three acoustic parameters (jitter, shimmer, and normalized noise energy), and two electroglottographic parameters (contact quotient and contact quotient perturbation) for vowels produced at three vocal efforts (low, normal, high). Data were obtained with use of a sophisticated bilinear interpolation pitch detection method. A repeated measures design required subjects to produce the vowels /ae/ and /a/ five times a day over 3 days at each vocal effort level. The jitter, shimmer, and normalized noise energy values from acoustic measures and contact quotient and contact quotient perturbation values varied significantly among the three vocal effort levels. The clinical implication of this finding is that vocal effort must be controlled in order to obtain consistent clinical measures. Furthermore, day-to-day variability must be taken into account if representative measures are to be obtained for clinical use.

Electric Stimulation↗
PubMed1994

[Preparation of digoxigenin labelled probe and detection of HBV DNA in liver and extrahepatic tissue with in situ hybridization].

A new rapid technique for intrahepatic and extrahepatic HBV DNA detection by using digoxigenin (dig) labelled probe with in situ hybridization was developed. This technique has the advantage of being non-radioactive and a quick procedure yielding stable results and showing a clear background. 45 liver specimens were tested with this technique. Among the patients with positive intrahepatic HBsAg and HBcAg, positive detection of HBV DNA was highest (77.27%, 17/22). Some results were confirmed by PCR test. 19 extrahepatic specimens were detected with in situ hybridization. HBV DNA was seen clearly in the nuclei of myocardial cells, pancreatic islet cell, renal tubule epithelial cells and testicular spermatogenic cells. The results of this study might contribute to the study of molecular mechanism of HBV-induced injury in liver cells and extrahepatic tissue.

Base Sequence↗
PubMed1994

[Detection of HBV DNA in kidney by PCR].

Nested polymerase chain reaction (PCR) in two groups of primer was performed in formalin-fixed, paraffin-embedded kidney and liver tissues from 11 autopsies of HBV infected patients for HBV DNA detection. DNA sequence representing HBV infection was detected in all the 11 liver tissue specimens (100%) and in 8 kidney tissue specimens (72.7%). DNA sequence representing HBV replication was detected in only 5 liver tissue specimens. The PCR findings correspond with those obtained in immunohistochemistry studies and in situ hybridization, suggesting that HBV can infect the kidney but does not replicate in this organ and that the kidney pathology in HBV infected patients may be the result of immuno-intermediate injury from immunocomplex deposited in glomeruli.

Base Sequence↗
PubMed1994

[Detection of HBV DNA in extrahepatic tissue with PCR comparison with immunohistochemistry and in situ hybridization].

Formalin-fixed paraffin-embedded tissue, including gallbladder, kidney, spleen, adrenal gland, heart, testicle, pancreas, and liver from eighteen autopsied cases with HBV infection were studied with nested polymerase chain reaction (PCR) for detection of HBV DNA. The DNA sequence representing HBV infection was detected in the tissue of liver (100%), gallbladder (6 from 7, 85.7%), spleen (6 from 8, 75.0%), kidney (8 from 11, 72.7%) adrenal gland (4 from 6, 66.7%), heart (10 from 18, 55.6%), testicle (10 from 18, 55.6%), pancreas (6 from 11, 54.5%) respectively. The DNA sequence representing HBV replication was detected in 5 cases of liver tissue only. The findings of PCR was correlated with the result of immunohistochemistry and in situ hybridization. It is shown that HBV can infect extrahepatic tissue but do not replicate in it. We think these findings may explain that the harboring of hepatitis virus in extrahepatic tissue could serve as one of extrahepatic infective sources, but have little pathological consequence on the infected extrahepatic organs.

Base Sequence↗
PubMed1991

Dendrolimiasis: an analysis of 58 cases.

Contact with pine caterpillars in China may result in dendrolimiasis. In this review of 58 cases the disease is considered from several aspects. Five types of disease are described, depending on the pathogenic site. Three stages could be recognized in relation to the time of onset. On the basis of an analysis of prominent theories, multiple factors are believed to be involved in the pathogenesis which involves damage to bones and joints. The radiographic features are outlined and the diagnosis and differential diagnosis discussed.

Adolescent↗
PubMed1990

[Hepatitis D virus RNA in serum from patients with hepatitis B].

To clarify the correlation of hepatitis D virus (HDV) infection and viral replication in liver diseases, the authors detected HDV RNA and serological HDV markers in serum from 285 patients with hepatitis B and 45 asymptomatic carriers of HBsAg. With dot blot hybridization, serum HDV RNA was detected in 8.8% (29/330) of the patients with HBV infection. The positive rate of HDV RNA in fulminant hepatitis was higher than that in benign hepatitis (15/74 vs 3/47, P less than 0.05). 10 of the 139 patients with chronic active hepatitis and 1 of the 6 cases with cirrhosis were positive for HDV RNA. However, all of the 19 cases with chronic persistent hepatitis and 45 asymptomatic carriers of HBsAg were negative fo, HDV RNA. Serological HDV markers, HDAgr anti-HD and IgM-anti-HD, were determined with ELISA. HDV RNA was detected in all of the serum samples with positive HDAg and/or IgM-anti-HD, in 15 of the 26 cases with positive-anti-HD and in 8 cases without HDV markers. Our results showed that 40 of the 330 patients with HBsAg were infected by HDV. This investigation suggests that HDV is one of the etiological factors for fulminant hepatitis and chronic active hepatitis.

Adolescent↗