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Biomedical subjects

D Zhang

Publications and source records attributed to D Zhang.

At least 451 records · Page 25Linked to original sources
PubMed1997

[The experimental study about the relations between Chinese herb-epimedium leptorrhizum stearn (CH-ELS) and endogenous cAMP in alveolar bone of orthodontic tooth in rats].

The aim of this study was, to explore the mechanism of CH-ELS promoting bone remodelling of orthodontic tooth in rats. 11 male sprague-Dawleg rats was divided into 3 groups. Group I (normal control group) Group II (applied force, no injection) Group III (applied force, injection). The experimental period was 3 days. The radioimmunoassay (RIA) method were used to investigate the effects of CH-ELS on the content of endogenous cAMP in alveolar bone of orthodontic tooth in rats. Results indicate that CH-ELS has no significant effects on endogenous cAMP in alveolar bone of orthodontic tooth, perhaps, cAMP were not involved to regulate the mechanism of CH-ELS promoting bone remodelling. To explore the mechanism of bone remodelling of orthodontic tooth is more significant in orthodontic clinic.

Alveolar Process↗
PubMed1997

[Scopes and applications of LeFort III advancement osteotomy].

In order to correct craniofacial retrusion and malocculusion caused by Crouzon syndrome, we employed LeFort III advancement osteotomies in 21 cases in last 10 years. The differences between craniofacial appearances and cephalometric analyses pre- and postoperatively showed that good results were achieved in all cases. No severe complications in these series were found.

Adolescent↗
PubMed1997

[Chemical constitutents of Podocarpus imbricatus BI. (II)].

From Podocarpus imbricatus five compounds were isolated and identified as 2"-O-rhamnosylvitexin (I), hinokiflavone (II), ecdysterone (III), beta-sitosteryl heptadecoate (IV) and daucosterol(V) on the basis of spectral studies (UV, IR, 1HNMR, 13CNMR, MS) and chemical reactions. I, II, III, IV are obtained from this plant for the first time, and IV is obtained as beta-sitosteryl odd number carboxylic ester for the first time.

Biflavonoids↗
PubMed1997

[Intrathoracic gastric function after esophagectomy for patients with esophageal cancer].

OBJECTIVE: To reveal the physiological changes of intrathoracic stomach after esophagectomy and find ways to improve patients' digestive function. METHODS: In twenty preoperative and 41 postoperative patients, the physiologic functions of stomach were studied and compared with radioisotope technique, pressure measurement, 24-hour pH monitoring and gastroscopy. RESULTS: The emptying of semisolid food from the intrathoracic stomach was much delayed (P < 0.01); its acid secretion was decreased in the early postoperative period (P < 0.01) but it restored to normal one year later. Fasting serum gastrin level was higher (P < 0.01) in the early postoperative period but showed a partial restoration one year later. The high pressure zone at anastomosis helped prevent gastroesophageal reflux. High incidence of gastritis was found in intrathoracic stomach and this may be related to gastric stasis. CONCLUSION: Performing scarf telescoping anastomosis and pyloraplasty, giving a certain amount of pepsin, diluted hydrochloric acid and anti-gastrin drug to early postoperative patients should be beneficial to reducing the symptoms and normalizing patients' digestive function.

Esophageal Neoplasms↗
PubMed1997

[Comparison of 4 extraction methods of chemical constituents in medicinal tea sishen chaji].

Four extraction method for the medicinal tea Sishen Chaji, were compared with psoralen, schizandrin B and evodiamine taken as indexes. The result shows that the total contents of the three compounds decrease progressively in the following order: semi-bionic extraction, semi-bionic extraction by precipitation with alcohol, extraction with water, and extraction with water and precipitation with alcohol.

Cyclooctanes↗
PubMed1997

[Pharmacological studies of luohanguo yanhou tablet].

Luohanguo Yanhou Tablet possesses the action of cotton ball granuloma of mice and the auricular edema induced by xylol, and also inhibiting the swelling of rat hind paw induced by carrageenin. The tests of mice writhing induced by acetic acid showed that the preparation had obvious analgesic action. It also possesses the obvious bacteriostatic action in vitro on five kinds of strains.

Analgesics↗
PubMed1997

[Determination of plasma drug concentration and bioavailability of indomethacin controlled release capsule by high performance liquid chromatography].

A simple, rapid, sensitive and accurate reversed-phase high performance liquid chromatographic (RP-HPLC) method for the determination of indomethacin in plasma is established. To 1 mL of plasma were added 200 microL of amobarbitone solution (internal standard; 1 g/L), 100 microL of phosphate buffer solution (pH 7.0), 100-150 mg of sodium chloride and 2.5 mL of ethyl acetate. The mixture was vortex-mixed for 10 min and centrifuged at 3000 r/min for 5 min. The organic layer was evaporated to dryness at 50 degrees C on water-bath and the residue was dissolved in methanol (100 microL). A 20 microL portion of the resulting solution was analysed by HPLC on a column (25 cm x 4.6 mm) of micro-Bondapak C18 (10 microm) with 76% methanol in 0.035 mol/L phosphoric acid (pH 5.5) aq-solution as mobile phase (1 mL/min) and UV detection at 260 nm. The column temperature was 30 degrees C. Within the range of 0.125-50 mg/L of indomethacin there was a good linearity (r = 0.9996). The mean recovery of indomethacin was 100.4%. The detectable limit was 62.5 microg/L (S/N = 3:1). The within day and day-to-day RSD were less than 5% at three drug levels. This method has been used to determine plasma drug concentration and bioavailability of indomethacin controlled release capsule for healthy volunteers.

Anti-Inflammatory Agents, Non-Steroidal↗
PubMed1997

Detection of rubella, mumps, and measles virus genomic RNA in cells from synovial fluid and peripheral blood in early rheumatoid arthritis.

OBJECTIVE: To determine, by studying patients with early rheumatoid arthritis (RA), whether rubella virus (rubella), mumps virus (mumps), or measles virus (measles) plays a role in the pathogenesis of RA. METHODS: Polymerase chain reaction combined with reverse transcription was used to detect viral RNA in peripheral blood mononuclear cells (PBMC) or synovial fluid (SF) cells. The patients with RA had newly diagnosed disease (duration < or = 1 year). The controls were patients with other arthropathies. RESULTS: Rubella genomic RNA was not detected in SF cells from patients with early RA or from controls, or in PBMC from patients with RA. It was found in PBMC of one of 46 patients with other arthropathies. Mumps or measles genomic RNA was detected in PBMC samples from 1.8% (1/54) and 9.3% (5/54) of RA, respectively, and from 4.3% (2/46) and 6.5% (3/46) of control patients. The SF cell samples harbored mumps or measles RNA in 4.8% (2/42) and 7.1% (3/42) of patients with RA, respectively; the corresponding value was 6.5% (2/31) for control patients, for both mumps and measles. CONCLUSION: Our findings suggest rubella, mumps, or measles do not play a role in the etiopathogenesis of RA.

Adult↗
PubMed1997

[Chemotherapy repeated injection via pelvic retroperitoneal cannulation].

OBJECTIVE: To investigate the feasibility of chemotherapy for lymphatic metastases of gynecologic cancer by repeated injection via pelvic retroperitoneal cannulation. METHODS: 5-fluorouracil (5-FU), thiotepa, methofrexate were repeatedly injected into the pelvic retroperitoneal space of 25 dogs and 24 patients respectively via cannulation. Microscope and high-performance liquid chromatography were used to observe the drug concentration of 5-FU and pathologic changes of the involved tissues. RESULTS: Animal experiment showed no toxicity to vessels, ureter and the normal tissues involved; nodal 5-FU concentration of repeated injection side was 7 to 9 times of that on the peripheral connective tissue (P < 0.05). Clinical study revealed: (1) the external iliac, hypogastric, obturator, deep inguinal and most of the common iliac lymph nodes were submerged in the 5-FU solution; (2) pelvic and periaortic nodal 5-FU concentrations of repeated injection side were 20 to 73 and 27 to 110 times of that on single injection side respectively; nodal 5-FU concentration was 11 to 20 times of that on the peripheral connective tissue; (3) metastatic cancer cells of nodal in the study group showed obvious degeneration and necrosis while no changes in the control; (4) no severe complications or adverse effects were observed. CONCLUSION: Repeated injection via pelvic retroperitoneal cannulation was feasible and probably more effective for the treatment of lymphatic metastases of gynecologic cancer.

Animals↗
PubMed1997

[Elimination of nitrogen dioxide and nitric oxide by soda lime and its components].

OBJECTIVES: To investigated the efficacy of soda lime and its components in absorbing NO2 and NO. METHODS: VIP BIRD ventilator was connected with Siemens test lung. While the concentration of inhaled NO was 80 mg/L, the inspired gas passed through soda lime or its component. NO and NO2 were measured before and after testing materials in the circuit. Test 1, the soda lime canister sequentially was filled with smaller particle soda lime 500, 250, 125, 62.5, 39, 20, 10 and 0 g, to determine weight-efficacy relationship of the soda lime. Test 2, the canister sequentially was filled with bigger particle soda lime 500, 62.5, 30, 20 and 10 g, for comparing effective absorption of NO and NO2 between the two types of soda lime Test 3, 1.0 g methyl orange was added to smaller particle soda lime 10 g and 20 g respectively to observe its effect on eliminating NO and NO2. Test 4, silica gel tube was filled with smaller particle soda lime 10 g, KOH 10 g, NaOH 10 g, Ca(OH)2 2.5 g, and methly orange 0.5 g respectively for observing their ability of eliminating NO and NO2. RESULTS: The two types soda lime had the ability to eliminating NO and NO2 from inspired gas with a positive relationship with its weight. Both KOH and NaOH did not eliminate NO and NO2 Ca(OH)2 and methyl orange had a week ability. Methyl orange did not affect the ability of soda lime to eliminate NO2, but decreased its ability to eliminate NO. During 240 min, the eliminating NO2 ability of soda lime did not change, but the eliminating NO ability was reduced from 30% to 19% after 60 min. CONCLUSIONS: The effective components to eliminate NO and NO2 in soda lime are Ca(OH)2 and NaOH. Their ability to eliminate NO and NO2 increases with increase in their weight. The ability to eliminate NO decreases with increase in the contacting time with NO.

Calcium Compounds↗
PubMed1997

[Activating blood circulation and removing stasis in treating polycythemia vera: clinical observation and therapeutical mechanism].

OBJECTIVE: To assess the efficacy of "method of activating blood circulation and removing stasis (ABCRS)" in treating polycythemia vera. METHODS: 28 cases of polycythemia vera were treated with ABCRS and observed with bone marrow colonies. RESULTS: 15 casses were responsibe, of them 4 cases reached clinical remission, 11 cases were improved. Both bone marrow colonies of colony forming unit-erythroid dependent of erythropoietin (EPO + CFU-E) and colonies of CFU-E independent of EPO. (EPO-CFU-E) from these cases decreased significantly, especially the latter. ABCRS mainly suppressed the proliferation and differentiation of EPO-CFU-E. In addition, bone marrow colonies of colony formng unit-fibroblastoids (CFU-F) from these cases also changed obviously. CONCLUSION: ABCRS is effective in treating polycythemia vera.

Adult↗
PubMed1997

[A preliminary study on effect of electro-acupuncture by the cortical infrared thermography].

OBJECTIVE: To explore the possibility of using cortical infrared thermography (CIT) and temperature in studying the principle of acupuncture and moxibustion. METHODS: The changes of the cerebral cortical temperature during electro-acupuncture (EA) by CIT in twenty cats were observed. By opening skull before experiments the cortex of animals was revealed. The distribution of the cortical temperature of the awakend animals was studied by AGA Infrared Thermovision, the thermograms were analysed by computer. RESULTS: (1) The thermograms of the profile of the cortex could be displayed clearly, the temperature was higher and lower in ante- and post-cortex respectively than that in other regions. (2) The cortical temperature declined slightly during 30 minutes in cats without EA. (3) After EA, the temperature of cortex generally increased 0.3 to 1.7 degrees centigrade, at 5 min after EA, the temperature increment was the highest, at 10 min after EA, the temperature increment was higher than before EA but lower than 5 min after EA, the cortical temperature recovered 10 min after stopping stimulating. (4) The temperature increment was more obvious in the regions of Ant. Ectosylvian, Ant. Suprasylvian and Ant. Sylvian than that in other regions. No matter where the site of EA was, every group had its region of higher temperature increment. The regions of the temperature increment in the cortex were mainly in the somatesthesia regions. There were similar characteristics between the temperature response in the cortex showing by CIT after EA and the activity of cortical nerve cells after stimulation. CONCLUSION: The display of the temperature response could be taken as the indication of the nervous activity. CIT could be used to conduct the studies of the acupuncture and brain.

Acupuncture Points↗
PubMed1997

[The application of distally based fascial pedicled island flap to rapair wound of the extremities].

In order to preserve the major vessels of the extremities in the repair and reconstruction of wounds of the extremities, the distally based fascial pedicled island flap was applied clinically. Its axis and rotatary point were designed along orientation of the major arteries, and the blood supply was from the abundant vascular networks in the deep fascia. Twenty-two cases with exposure of tendon and bone including 10 upper limbs and 12 lower limbs were treated. The flap area of forearm ranged from 7 cm x 8 cm to 12 cm x 9 cm and the ratio of the length to width of the pedicle was 1: 1-2. The flap area of the calf ranged from 10 cm x 6 cm to 16.5 cm x 12 cm and the ratio of the length to width of the pedicle was 2:1. The rotatary angle was 130 degrees-170 degrees. After operation, 18 flaps were survived completely, 2 cases had partial necrosis on the margin, 2 failures received cross-leg flap in the second operation. The patients were followed up with an average of 13.5 months (ranged from 3 months to 2 years). The conclusions were as follows: 1. the blood supply of this type of flap was reliable and the major arteries of the extremities needed not to be sacrificed; 2. the preparation of the flap was easy and the survival rate was satisfactory; 3. the shortcomings of this flap were unsightly incision scar and the limited size of flap and; 4. during the operation, the compression of the pedicle must be avoided.

Adolescent↗
PubMed1997

[Analysis of pure titanium implants-bone interface by TOF-SIMS].

In order to study the Titanium-bone interaction and integration mechanism, the titanium implant was implanted in the tibia of 9 Newzealand rabbits, and the Ti-bone interface performed for 1, 3, and 6 months were examined and analyzed by fluorescence microscope and advanced TOF-SIMS techniques. The results showed that Ti-bone tissue was integrated closely in a very reactive manner. Both physical and chemical integration occurred in the Ti-bone interface. The Ti-bone could diffuse into the bone tissue though the diffusion was very limited. It was up to 100 microns in depth during the early period. The diffusion density was high, and later in a smooth distribution. Furthermore, while Ti+ diffused into the bone tissue, other elements such as Ca+, OH-, O-, etc, could also diffuse into titanium in exchange. The growth pattern around the bone tissue was in two fashions, one was implantefugal and the other was implantopetal. In this study, based on the ionic distribution, osmosis and impurity elements distribution, the Ti-bone integration mechanism was discussed at molecular and atomic level.

Animals↗